Herpes virus infection prevalence in regular haemodialysis patients--a comparative evaluation of complement fixation, indirect immunofluorescence and Elisa tests.

Proceedings of the European Dialysis and Transplant Association - European Renal Association. European Dialysis and Transplant Association - European Renal Association. Congress Pub Date : 1985-01-01

F Scolari, N Manca, S Sandrini, L Cristinelli, E Prati, G Setti, S Savoldi, A Turano, R Maiorca

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Abstract

The presence and titres of specific serum IgG and IgM antibodies to cytomegalovirus, herpes simplex virus and varicella-zoster virus were evaluated in 50 haemodialysis patients by complement fixation, immunofluorescence and Elisa tests. A second serum sample was tested in 24 patients after four weeks. Specific serum IgG antibodies to Epstein-Barr virus were also measured by immunofluorescence in 26 patients. By immunofluorescence and Elisa tests, the prevalence of cytomegalovirus, herpes simplex virus and Epstein-Barr virus infection is approximately 100 per cent, and varicella-zoster virus 60 per cent. High titres of IgG specific antibodies found by Elisa tests, detection of IgM antibodies in 16-18 per cent of patients and sero-conversion in 25 per cent of patients, suggests continuous antigenic stimulation.

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常规血液透析患者疱疹病毒感染的流行——补体固定、间接免疫荧光和Elisa试验的比较评价

采用补体固定法、免疫荧光法和Elisa法检测50例血液透析患者血清巨细胞病毒、单纯疱疹病毒和水痘-带状疱疹病毒特异性IgG和IgM抗体的存在和滴度。在4周后，对24名患者进行了第二次血清样本检测。用免疫荧光法检测26例患者血清中eb病毒特异性IgG抗体。通过免疫荧光和Elisa试验，巨细胞病毒、单纯疱疹病毒和eb病毒感染的流行率约为100%，水痘带状疱疹病毒感染的流行率为60%。Elisa试验发现IgG特异性抗体高滴度，16- 18%的患者检测到IgM抗体，25%的患者血清转化，表明持续的抗原刺激。

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Proceedings of the European Dialysis and Transplant Association - European Renal Association. European Dialysis and Transplant Association - European Renal Association. Congress

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