Proteomic profiling of human dental enamel affected by molar incisor hypomineralisation of different clinical severity grades: an in vitro study.

IF 2.3 Q2 DENTISTRY, ORAL SURGERY & MEDICINE European Archives of Paediatric Dentistry Pub Date : 2024-08-01 Epub Date: 2024-06-06 DOI:10.1007/s40368-024-00911-9

F Rexhaj, N Sabel, A Robertson, T Lundgren

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Abstract

Purpose: The aim of this study was to explore the potential to profile and distinguish varying clinical severity grades of MIH, compared to normal enamel, using proteomics.

Methods: Liquid chromatography-mass spectrometry analyses were conducted on enamel samples of extracted teeth, from 11 children and adolescents, spanning an age range of 6-18 years. Enamel powder samples were collected from extracted, third molars (n = 3) and first permanent molars diagnosed with MIH (n = 8). The MIH tooth samples were categorized into subgroups based on clinical severity grade. The data were statistically analyzed using ANOVA and Welch's t test.

Results: Teeth affected by MIH exhibited a diverse array of proteins, each with different functions related to dental enamel, distinguishing them from their normal enamel counterparts. The application of microdissection combined with LC-MS techniques has revealed the potential to discern unique proteomic profiles among MIH-affected teeth, characterized by varying clinical severity grades. Both analyzed MIH groups displayed consistent trends in the presentation of biological processes, including underabundance of proteins primarily associated with cell organization and biogenesis. Furthermore, proteins linked to cell death were overabundant in both MIH groups.

Conclusion: Proteomics enabled the detection and differentiation of various proteins across different clinical severity grades of MIH.

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不同临床严重程度的磨牙切牙低矿化所影响的人类牙釉质的蛋白质组分析：一项体外研究。

目的：本研究的目的是利用蛋白质组学的方法，探讨与正常牙釉质相比，对不同临床严重程度的MIH进行剖析和区分的可能性：对 11 名 6-18 岁儿童和青少年的拔牙珐琅质样本进行了液相色谱-质谱分析。珐琅质粉末样本取自被诊断为MIH的拔出第三磨牙（3颗）和第一恒磨牙（8颗）。根据临床严重程度将 MIH 牙齿样本分为不同的亚组。采用方差分析和韦尔奇 t 检验对数据进行统计分析：结果：受MIH影响的牙齿表现出多种多样的蛋白质，每种蛋白质都具有与牙釉质相关的不同功能，使它们有别于正常的牙釉质。显微切割结合 LC-MS 技术的应用揭示了在受 MIH 影响的牙齿中发现独特蛋白质组图谱的潜力，这些图谱以不同的临床严重程度等级为特征。两组受分析的 MIH 患者在生物过程的表现上呈现出一致的趋势，包括主要与细胞组织和生物生成相关的蛋白质含量不足。此外，与细胞死亡有关的蛋白质在两组 MIH 中都过量存在：蛋白质组学能够检测和区分不同临床严重程度等级的 MIH 中的各种蛋白质。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Archives of Paediatric Dentistry DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

4.40

自引率

9.10%

发文量

期刊介绍： The aim and scope of European Archives of Paediatric Dentistry (EAPD) is to promote research in all aspects of dentistry for children, including interceptive orthodontics and studies on children and young adults with special needs. The EAPD focuses on the publication and critical evaluation of clinical and basic science research related to children. The EAPD will consider clinical case series reports, followed by the relevant literature review, only where there are new and important findings of interest to Paediatric Dentistry and where details of techniques or treatment carried out and the success of such approaches are given.