Pengaruh Ekstrak Buah Rimbang (Solanum torvum Sw.) Terhadap Pertumbuhan Bakteri Enterococcus faecalis

Abstract

Abstract: Root canal treatment is performed to eliminate causal factors of infection and remove necrotic tissue. However, during treatment, the persistence of the microorganism, Enterococcus faecalis, proved to be the cause of treatment failure. Currently a lot of research is being done on natural materials as an alternative to irrigation materials because they are considered to be more biocompatible and economical; one of which is the Turkey berry fruit plant (Solanum torvum Sw.). This study aimed to evaluate the effects of several concentrations of Turkey berry fruit extract on the growth of Enterococcus faecalis through minimum inhibitory and minimum lethal concentrations (MIC and LCmin). This was a post test only control group design study. Samples were divided into treatment and control groups:  12,5%, 25%, 50% dan 100% extract of Turkey berry fruit, chlorhexidine as the positive control, and 0.9% NaCl as the negative control. All of them had been implanted with bacterial suspension, and then were spread on the MHA media. Observations were performed after 24 hours of incubation, and bacterial colony were count manually. Data were analyzed using the non parametric Kruskal-Wallis test and the Tamhane’s post hoc test that showed significant difference among the groups with MIC at 25% and LCmin at 50%. In conclusion, Turkey berry fruit extract of 25% and 50% can inhibit the growth of Enterococcus faecalis. Keywords: root canal treatment; chlorhexidine; Enterococcus faecalis; Turkey berry fruit   Abstrak: Perawatan saluran akar dilakukan untuk mengeliminasi faktor penyebab infeksi dan menghilangkan jaringan nekrotik. Persistensi mikroorganisme yaitu Enterococcus faecalis terbukti menjadi penyebab kegagalan perawatan. Saat ini banyak penelitian dilakukan terhadap bahan alami sebagai alternatif bahan irigasi karena dinilai lebih biokompatibel dan ekonomis, salah satunya tanaman buah rimbang (Solanum torvum Sw.). Penelitian ini bertujuan untuk mengetahui pengaruh tingkat konsentrasi ekstrak buah rimbang (Solanum torvum Sw.) terhadap pertumbuhan bakteri Enterococcus faecalis melalui pengamatan nilai konsentrasi hambat minimal (KHM) dan konsentrasi bunuh minimal (KBM) dengan menggunakan post test only control group design. Sampel penelitian dibagi atas kelompok perlakuan dan kontrol, yaitu ekstrak buah rimbang konsentrasi 12,5%, 25%, 50% dan 100% serta kontrol negatif dan positif yang telah ditanamkan suspensi bakteri disebarkan pada media MHA. Pengamatan dilakukan setelah inkubasi selama 24 jam dan perhitungan koloni bakteri secara manual. Data dianalisis menggunakan uji non parametrik Kruskal-Wallis dan uji Tamhane Post Hoc yang menunjukkan perbedaan bermakna antar kelompok dengan KHM pada konsentrasi 25% dan KBM pada konsentrasi 50%. Simpulan penelitian ini ialah ekstrak buah rimbang pada konsentrasi 25% dan 50% dapat menghambat pertumbuhan Enterococcus faecalis. Kata kunci: perawatan saluran akar; chlorhexidine; Enterococcus faecalis; buah rimbang