Unveiling molecular mechanisms of pigment synthesis in gardenia (Gardenia jasminoides) fruits through integrative transcriptomics and metabolomics analysis

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY Food Chemistry Molecular Sciences Pub Date : 2024-06-03 DOI:10.1016/j.fochms.2024.100209
Kangqin Li , Lixin Yu , Liqin Gao , lingzhi Zhu , Xiaotao Feng , Shaoyong Deng
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Abstract

This study conducted a combined transcriptomics and metabolomics analysis in premature and mature developmental stages of Gardenia jasminoides Ellis fruits to identify the molecular mechanisms of pigment synthesis. The transcriptomics data produced high-quality clean data amounting to 46.98 gigabytes, exhibiting a mapping ratio of 86.36% to 91.43%. Transcriptomics analysis successfully identified about 3,914 differentially expressed genes which are associated with pivotal biological processes, including photosynthesis, chlorophyll, biosynthetic processes, and protein-chromophore linkage pathways. Functional diversity was clarified by the Clusters of Orthologous Groups (COG) classification, which focused mainly on pigment synthesis functions. Pathways analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) revealed critical pathways affecting pigment development. Metabolomics studies were carried out utilizing Ultra Performance Liquid Chromatography and mass spectrometry (UPLC-MS). About 480 metabolites were detected via metabolomics investigation, the majority of that were significantly involved in pigment synthesis. Cluster and pathway analyses revealed the importance of pathways such as plant secondary metabolite biosynthesis, biosynthesis of phenylpropanoids and plant hormone signal transduction in pigment synthesis. Current research advances our comprehension of the underlying mechanisms at the molecular level governing pigment synthesis in gardenia fruits, furnishing valuable insights for subsequent investigations.

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通过整合转录组学和代谢组学分析揭示栀子果实色素合成的分子机制
本研究对栀子果实的早熟和成熟发育阶段进行了转录组学和代谢组学联合分析,以确定色素合成的分子机制。转录组学数据产生了46.98千兆字节的高质量纯净数据,其映射比为86.36%至91.43%。转录组学分析成功鉴定了约 3,914 个差异表达基因,这些基因与关键的生物过程有关,包括光合作用、叶绿素、生物合成过程和蛋白质-色素连接途径。功能多样性通过同源组簇(COG)分类得以明确,主要集中在色素合成功能上。利用京都基因和基因组百科全书(KEGG)和基因本体(GO)进行的通路分析揭示了影响色素发育的关键通路。利用超高效液相色谱和质谱法(UPLC-MS)进行了代谢组学研究。通过代谢组学研究,共检测到约 480 种代谢物,其中大部分与色素合成有重要关系。聚类和通路分析表明,植物次生代谢物的生物合成、苯丙类化合物的生物合成和植物激素信号转导等通路在色素合成中具有重要作用。目前的研究加深了我们对栀子果实色素合成分子水平基本机制的理解,为后续研究提供了宝贵的见解。
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来源期刊
Food Chemistry Molecular Sciences
Food Chemistry Molecular Sciences Agricultural and Biological Sciences-Food Science
CiteScore
6.00
自引率
0.00%
发文量
83
审稿时长
82 days
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