Research on the pharmacognostic characteristics, physicochemical properties and in vitro antioxidant potency of Rosa laxa Retz. flos

IF 2 3区工程技术 Q2 ANATOMY & MORPHOLOGY Microscopy Research and Technique Pub Date : 2024-06-10 DOI:10.1002/jemt.24622

Ying Guo, Wenhui Zhao, Yuan He, Anling Li, Qianqian Feng, Li Tian

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Abstract

Rosa laxa Retz. is an unexplored Rosaceae plant in Xinjiang, China, and its flower is traditionally used in Kazak to treat the common cold, fever, and epileptic seizures and lessen the effects of aging. In the present study, the pharmacognostic profiles, physicochemical properties, phytochemical characteristics, and in vitro antioxidant potency of Rosa laxa Retz. flos (RLF) were presented. In the pharmacognostic evaluation of RLF, organoleptic characteristics, internal structures, and powder information were observed, and physicochemical parameters, including moisture content, ash, pH value, swelling degree, and extractives were examined. The quantitative analysis of the chemical composition of four different polar extracts of RLF showed that the aqueous part had the highest total triterpene acid, flavonoid, and polyphenol content (4.50 ± 0.04 mg/g, 50.56 ± 0.03 mg/g, and 60.20 ± 0.09 mg/g, respectively). A high-performance liquid chromatography (HPLC)-diode array detector (DAD) method was established and the contents of gallic acid, ellagic acid, astragalin, and tiliroside in RLF were determined simultaneously. In the set concentration range, the linear relationship among the four components was good (r > 0.999), the average recoveries were 97.36%–100.54%. The contents of gallic acid, ellagic acid, astragalin, and tiliroside in RLF samples were (9.46 ± 2.31) mg/g, (10.60 ±0.75) mg/g, (1.13 ± 2.50) mg/g, and (1.11 ± 2.65) mg/g, respectively. The types of its secondary metabolites were determined by fluorescence, color reaction by chemical solvent method, and ultraviolet–visible (UV–Vis) spectroscopy. The functional groups of its secondary metabolites were determined by Fourier transform infrared (FTIR) spectroscopy. Results showed that RLF contains a variety of secondary metabolic products, including flavonoids, phenolic acid, glycoside, and organic acid. TLC identification showed it contains ursolic acid, β-sitosterol, tiliroside, astragalin, isoquercitrin, kaempferol-3-O-rutinoside, gallic acid, and ellagic acid. The in vitro antioxidant activity of different polar parts of RLF was investigated by DPPH, ABTS, and reduction performance experiments. The aqueous extract had the strongest antioxidant capacity, consistent with the high content of triterpene acids, flavonoids, and polyphenolic compounds. These findings will provide critical information for the study of quality standards and medicinal value of RLF and its extracts, justify its usage in traditional medicinal systems, and encourage the use of this plant in disease prevention and treatment. Its phytochemical composition and pharmacological studies need to be explored in future.

Research Highlights

Optical microscope and scanning electron microscope (SEM) were used to observe the morphology, and microstructure of Rosa laxa Retz. flos (RLF).
The physicochemical properties, fluorescence and phytochemical composition of four different polar extracts of RLF were analyzed by UV–Vis and FTIR.
Determination of total triterpenic acid, total flavonoids, and total polyphenols in four different polar extracts of RLF by UV spectrophotometry.
A high-performance liquid chromatography (HPLC)-diode array detector (DAD) method was established and the contents of gallic acid, ellagic acid, astragalin, and tiliroside in RLF were determined simultaneously. TLC confirmed that RLF contains ursolic acid, β-sitosterol, tiliroside, astragalin, isoquercitrin, kaempferol 3-rutinoside, gallic acid, and ellagic acid.
The in vitro antioxidant activity of RLF was studied by DPPH, ABTS, and reducing ability experiments.

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Rosa laxa Retz.

喀什蔷薇（Rosa laxa Retz.）是中国新疆地区一种尚未开发的蔷薇科植物，喀什地区传统上使用它的花来治疗普通感冒、发烧、癫痫发作和减轻衰老的影响。本研究介绍了蔷薇花（RLF）的药理特征、理化性质、植物化学特性和体外抗氧化效力。在 RLF 的药理评价中，观察了其感官特征、内部结构和粉末信息，并考察了其理化指标，包括水分含量、灰分、pH 值、膨胀度和提取物。四种不同极性提取物的化学成分定量分析结果表明，水提取物中三萜酸、黄酮和多酚的总含量最高（分别为 4.50 ± 0.04 mg/g、50.56 ± 0.03 mg/g 和 60.20 ± 0.09 mg/g）。建立了高效液相色谱-二极管阵列检测器（DAD）分析方法，同时测定了 RLF 中没食子酸、鞣花酸、黄芪苷和枸橘苷的含量。在设定的浓度范围内，四种成分的线性关系良好（r > 0.999），平均回收率为 97.36%-100.54%。没食子酸、鞣花酸、黄芪苷和桔梗苷的含量分别为（9.46 ± 2.31）毫克/克、（10.60 ± 0.75）毫克/克、（1.13 ± 2.50）毫克/克和（1.11 ± 2.65）毫克/克。次生代谢物的种类是通过荧光、化学溶剂法颜色反应和紫外可见光谱（UV-Vis）测定的。次生代谢物的官能团是通过傅立叶变换红外光谱（FTIR）测定的。结果表明，RLF 含有多种次生代谢产物，包括黄酮类、酚酸、苷和有机酸。TLC鉴定显示，它含有熊果酸、β-谷甾醇、枸杞苷、黄芪苷、异槲皮苷、山奈酚-3-O-芸香苷、没食子酸和鞣花酸。通过 DPPH、ABTS 和还原性实验研究了 RLF 不同极性部分的体外抗氧化活性。水提取物的抗氧化能力最强，这与三萜酸、类黄酮和多酚类化合物的高含量是一致的。这些发现将为研究 RLF 及其提取物的质量标准和药用价值提供重要信息，证明其在传统医学体系中的应用是合理的，并鼓励在疾病预防和治疗中使用这种植物。今后还需要对其植物化学成分和药理研究进行探索。研究重点：使用光学显微镜和扫描电子显微镜（SEM）观察蔷薇花（RLF）的形态和微观结构。利用紫外可见光和傅立叶变换红外光谱分析了四种不同极性萃取物的理化性质、荧光和植物化学成分。用紫外分光光度法测定 RLF 四种不同极性提取物中的总三萜酸、总黄酮类化合物和总多酚。建立了高效液相色谱（HPLC）-二极管阵列检测器（DAD）方法，同时测定了 RLF 中没食子酸、鞣花酸、黄芪苷和枸橘苷的含量。TLC 证实 RLF 中含有熊果酸、β-谷甾醇、桔梗苷、黄芪苷、异槲皮苷、山奈酚 3-芸香苷、没食子酸和鞣花酸。通过 DPPH、ABTS 和还原能力实验研究了 RLF 的体外抗氧化活性。

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来源期刊

Microscopy Research and Technique 医学-解剖学与形态学

CiteScore

5.30

自引率

20.00%

发文量

233

审稿时长

4.7 months

期刊介绍： Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.