Isolation and characterization of a phage collection against Pseudomonas putida

IF 4.3 2区 生物学 Q2 MICROBIOLOGY Environmental microbiology Pub Date : 2024-06-11 DOI:10.1111/1462-2920.16671
Age Brauer, Sirli Rosendahl, Anu Kängsep, Alicja Cecylia Lewańczyk, Roger Rikberg, Rita Hõrak, Hedvig Tamman
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Abstract

The environmental bacterium, Pseudomonas putida, possesses a broad spectrum of metabolic pathways. This makes it highly promising for use in biotechnological production as a cell factory, as well as in bioremediation strategies to degrade various aromatic pollutants. For P. putida to flourish in its environment, it must withstand the continuous threats posed by bacteriophages. Interestingly, until now, only a handful of phages have been isolated for the commonly used laboratory strain, P. putida KT2440, and no phage defence mechanisms have been characterized. In this study, we present a new Collection of Environmental P. putida Phages from Estonia, or CEPEST. This collection comprises 67 double-stranded DNA phages, which belong to 22 phage species and 9 phage genera. Our findings reveal that most phages in the CEPEST collection are more infectious at lower temperatures, have a narrow host range, and require an intact lipopolysaccharide for P. putida infection. Furthermore, we show that cryptic prophages present in the P. putida chromosome provide strong protection against the infection of many phages. However, the chromosomal toxin–antitoxin systems do not play a role in the phage defence of P. putida. This research provides valuable insights into the interactions between P. putida and bacteriophages, which could have significant implications for biotechnological and environmental applications.

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针对假单胞菌(Pseudomonas putida)的噬菌体集的分离和特征描述。
环境细菌普氏假单胞菌(Pseudomonas putida)具有广泛的代谢途径。这使它在生物技术生产中作为细胞工厂,以及在生物修复战略中降解各种芳香污染物方面大有可为。为了使 P. putida 在其生存环境中繁衍生息,它必须能够抵御噬菌体带来的持续威胁。有趣的是,到目前为止,只有少数噬菌体从常用的实验室菌株 P. putida KT2440 中分离出来,而且还没有噬菌体防御机制的特征。在本研究中,我们展示了一个新的《爱沙尼亚环境普氏菌噬菌体集》(或称 CEPEST)。该收集包括 67 个双链 DNA 噬菌体,隶属于 22 个噬菌体种和 9 个噬菌体属。我们的研究结果表明,CEPEST 收集的大多数噬菌体在较低温度下更具传染性,宿主范围较窄,并且需要完整的脂多糖才能感染普氏菌。此外,我们还发现腐生菌染色体中的隐性噬菌体对许多噬菌体的感染具有很强的保护作用。然而,染色体毒素-抗毒素系统并没有在腐生菌的噬菌体防御中发挥作用。这项研究为了解腐生菌与噬菌体之间的相互作用提供了有价值的见解,可能对生物技术和环境应用产生重大影响。
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来源期刊
Environmental microbiology
Environmental microbiology 环境科学-微生物学
CiteScore
9.90
自引率
3.90%
发文量
427
审稿时长
2.3 months
期刊介绍: Environmental Microbiology provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens
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