Prodelphinidins enhance dentin matrix properties and promote adhesion to methacrylate resin

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Dental Materials Pub Date : 2024-06-12 DOI:10.1016/j.dental.2024.05.024

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Abstract

Objective

Investigate the bioactivity and stability of Rhodiola rosea (RR) fractions as a natural source of prodelphinidin gallate (PDg) on dentin collagen via analysis of the viscoelastic and resin-dentin adhesive properties of the dentin matrix.

Methods

The biomimicry and stability of RR subfractions (F1, F2, F3 and F4) with collagen were determined by dynamic mechanical analysis (DMA). DMA used a strain sweep method to assess the dentin matrix viscoelastic properties [storage (E’), loss (E”), and complex (E*) moduli and tan δ] after treatment, 7-, 30- and 90-days of storage in simulated body fluids (SBF). Resin-dentin interface properties were assessed after 1 and 90-days in SBF by microtensile bond strength test and confocal laser scanning microscopy. Data were analyzed using two and one-way ANOVA and post-hoc tests (α = 0.05).

Results

RR fractions increased dentin matrix complex (96 - 69 MPa) and storage (95 - 68 MPa) moduli, compared to the control (∼9 MPa) in the ranking order: F2 ≥ F3 = F1 = F4 > control (p < 0.001). Treatment did not affect tan δ values. After 30- and 90-days, RR-treated dentin E*, E’ and tan δ decreased (p < 0.001). F2 fraction yielded the highest microtensile bond strength (43.9 MPa), compared to F1, F4 (35.9 - 31.7 MPa), and control (29 MPa). RR-treated interfaces mediated stable surface modifications and enhanced collagen-methacrylate resin interactions at the bioadhesive interface.

Significance

Prodelphinidin gallates from RR are potent and reasonably stable biomimetic agents to dentin. Higher potency of F2 fraction with the dentin matrix and the adhesive interface is associated with a degree of polymerization of 2–3 and gallo(yl) motifs.

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原鹅掌楸素能增强牙本质基质的特性，促进与甲基丙烯酸树脂的粘合。

目的通过分析牙本质基质的粘弹性和树脂-牙本质粘附性，研究作为天然原没食子酸盐（PDg）来源的红景天（RR）馏分对牙本质胶原的生物活性和稳定性：通过动态力学分析（DMA）确定了 RR 亚组分（F1、F2、F3 和 F4）与胶原蛋白的生物仿生性和稳定性。DMA 采用应变扫描法评估牙本质基质在模拟体液（SBF）中处理、储存 7 天、30 天和 90 天后的粘弹性能[储存模量（E'）、损失模量（E"）和复合模量（E*）以及 tan δ]。在 SBF 中存放 1 天和 90 天后，通过微拉伸粘接强度测试和共聚焦激光扫描显微镜对树脂-牙本质界面特性进行了评估。数据分析采用双向和单向方差分析及事后检验（α = 0.05）：与对照组（∼9 MPa）相比，RR馏分依次增加了牙本质基质复合模量（96 - 69 MPa）和储存模量（95 - 68 MPa）：F2 ≥ F3 = F1 = F4 > 对照组（p 显著性：来自 RR 的原没食子酸盐是对牙本质有效且相当稳定的生物仿生制剂。F2 部分与牙本质基质和粘合界面的较高效力与 2-3 和没食子酰基的聚合度有关。

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来源期刊

Dental Materials 工程技术-材料科学：生物材料

CiteScore

9.80

自引率

10.00%

发文量

290

审稿时长

67 days

期刊介绍： Dental Materials publishes original research, review articles, and short communications. Academy of Dental Materials members click here to register for free access to Dental Materials online. The principal aim of Dental Materials is to promote rapid communication of scientific information between academia, industry, and the dental practitioner. Original Manuscripts on clinical and laboratory research of basic and applied character which focus on the properties or performance of dental materials or the reaction of host tissues to materials are given priority publication. Other acceptable topics include application technology in clinical dentistry and dental laboratory technology. Comprehensive reviews and editorial commentaries on pertinent subjects will be considered.