Effect of Salicylic Acid on the gene expression of FnbA and FnbB genes in Staphylococcus hominis.

Q3 Medicine Human Antibodies Pub Date : 2024-01-01 DOI:10.3233/HAB-240023

Halah Ahmed Abdulqader, Zainab Hekmatt Abood

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引用次数: 0

Abstract

Background: Staphylococcus hominis is an opportunistic pathogen that expresses surface proteins, which are adhesive proteins that play a major role in biofilm formation. Biofilm is a protective layer that provides S. hominis bacteria with greater antibiotic resistance and promotes its adherence to biomedical surfaces, facilitating its entry into the bloodstream.

Objective: This research aimed to investigate the activity of Salicylic Acid (SA) and its effect on the gene expression of biofilm genes (FnbA and FnbB genes).

Methods: A total of 150 blood specimens were collected from patients. The specimens were cultured in broth media of the BacT/ALERT® system and subcultured on blood and chocolate agar. Bacteria were detected using the VITEK2 system. FnbA and FnbB genes were detected using PCR. The broth microdilution method performed the minimum inhibitory concentration (MIC) of Salicylic acid (SA) on S. hominis isolates with both genes. Detection of the gene expression levels of FnbA and FnbB genes was assessed using Real-Time PCR(RT-PCR).

Results: The results showed that out of the 150 specimens collected, 35 were S. hominis. The detection of S. hominis bacteria was performed by PCR amplification of two genes FnbA and FnbB and showed 100% and 17.14% of isolates were positive for genes FnbA and FnbB, respectively. The expression of FnbA and FnbB genes was decreased in samples treated with SA compared with untreated ones.

Conclusion: In conclusion, there is a significant impact of SA on the prevention of biofilm formation of S. hominis through the suppression of gene expression, specifically FnbA and FnbB. This could enhance susceptibility to antimicrobial treatments. However, more research is required to determine whether SA leads to the selection of resistant bacteria.

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水杨酸对人葡萄球菌 FnbA 和 FnbB 基因表达的影响

背景：人葡萄球菌是一种机会性病原体，它能表达表面蛋白，而表面蛋白是一种粘附蛋白，在生物膜形成过程中发挥着重要作用。生物膜是一种保护层，为人葡萄球菌提供了更强的抗生素耐药性，并促进其附着于生物医学表面，为其进入血液提供了便利：本研究旨在探讨水杨酸（SA）的活性及其对生物膜基因（FnbA 和 FnbB 基因）表达的影响：方法：共收集了 150 份患者血液标本。标本在 BacT/ALERT® 系统的肉汤培养基中培养，并在血液和巧克力琼脂上进行亚培养。使用 VITEK2 系统检测细菌。使用 PCR 检测 FnbA 和 FnbB 基因。肉汤微量稀释法测定水杨酸（SA）对带有这两种基因的人嗜血杆菌分离株的最低抑菌浓度（MIC）。采用实时 PCR（RT-PCR）技术检测 FnbA 和 FnbB 基因的表达水平：结果表明，在采集的 150 份标本中，有 35 份为人吸虫。通过对 FnbA 和 FnbB 两种基因进行 PCR 扩增，结果显示分别有 100%和 17.14%的分离株对 FnbA 和 FnbB 基因呈阳性。与未处理的样本相比，用 SA 处理的样本中 FnbA 和 FnbB 基因的表达量有所下降：总之，通过抑制基因（特别是 FnbA 和 FnbB）的表达，SA 对预防人乳头瘤病毒生物膜的形成有重要影响。这可能会提高对抗菌治疗的敏感性。不过，要确定 SA 是否会导致耐药细菌的产生，还需要进行更多的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Human Antibodies Medicine-Immunology and Allergy

CiteScore

3.50

自引率

0.00%

发文量

期刊介绍： Human Antibodies is an international journal designed to bring together all aspects of human hybridomas and antibody technology under a single, cohesive theme. This includes fundamental research, applied science and clinical applications. Emphasis in the published articles is on antisera, monoclonal antibodies, fusion partners, EBV transformation, transfections, in vitro immunization, defined antigens, tissue reactivity, scale-up production, chimeric antibodies, autoimmunity, natural antibodies/immune response, anti-idiotypes, and hybridomas secreting interesting growth factors. Immunoregulatory molecules, including T cell hybridomas, will also be featured.