Exploration of Anethum graveolens diversity from North Western Himalayan

IF 3.8 2区 农林科学 Q1 PLANT SCIENCES Journal of Applied Research on Medicinal and Aromatic Plants Pub Date : 2024-06-07 DOI:10.1016/j.jarmap.2024.100559
Rhydum Sharma , Richa Salwan , Naveen Dwivedi , Abhishek Kumar Singh , Vivek Sharma
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Abstract

Anethum graveolens L., an aromatic plant grown during winter season belongs to the family Apiaceae. The leaves and seeds of the plants are widely consumed in India. The seeds of the plants are rich in aroma, due to the richness of essential oils whereas the leaves hold immense potential due to their antioxidant and heavy metal stress alleviation properties. However, the genetic diversity of A. graveolens from North Western Himalayan has not been explored so far. Therefore, the genetic diversity of A. graveolens was investigated using 33 random amplified polymorphic (RAPD) and 27 inter sequence specific region (ISSR) markers. Out of the 33 random primers, only 4 RAPD markers resulted in PCR amplification whereas 18 ISSR markers resulted in reproducible PCR amplification. Out of these ISSR markers, 11 markers accounted for 75–100 % of the polymorphism across all the accessions. Additionally, protein profiling was also used to investigate the diversity of A. graveolens landraces. Overall, the cluster analysis using Neighbor joining (N-J) and unweighted pair group method with arithmetic mean (UPGMA) method followed by principal component analysis (PCA) revealed that the accessions of A. graveolens irrespective to their geographic distribution shared the common group. Furthermore, the antioxidant potential of the local landraces was also investigated based on the free radical scavenging potential using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The present current study on exploration of A. graveolens diversity can be helpful in developing conservation strategies of its local germplasm prevalent in North Western Himalayan region.

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喜马拉雅山西北部 Anethum graveolens 多样性探索
Anethum graveolens L.是一种在冬季生长的芳香植物,属于伞形科。这种植物的叶子和种子在印度被广泛食用。这种植物的种子因富含精油而香气浓郁,而叶子则因其抗氧化和缓解重金属压力的特性而潜力巨大。然而,迄今为止,喜马拉雅山西北部的 A. graveolens 的遗传多样性尚未得到探索。因此,我们使用 33 个随机扩增多态性(RAPD)和 27 个序列间特异性区域(ISSR)标记对 A. graveolens 的遗传多样性进行了研究。在 33 个随机引物中,只有 4 个 RAPD 标记能进行 PCR 扩增,而 18 个 ISSR 标记能进行可重复的 PCR 扩增。在这些 ISSR 标记中,有 11 个标记在所有品种的多态性中占 75-100%。此外,蛋白质分析也用于研究 A. graveolens 地方品种的多样性。总体而言,使用邻接法(N-J)和带算术平均值的非加权成对分组法(UPGMA)进行聚类分析,然后进行主成分分析(PCA)后发现,不论地理分布如何,A. graveolens 的登录品种都有一个共同的群体。此外,研究人员还使用 2,2-二苯基-1-苦基肼(DPPH)测定法,根据清除自由基的潜力对当地土种的抗氧化潜力进行了调查。本研究对 A. graveolens 多样性的探索有助于制定喜马拉雅山西北部地区当地种质的保护策略。
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来源期刊
Journal of Applied Research on Medicinal and Aromatic Plants
Journal of Applied Research on Medicinal and Aromatic Plants Pharmacology, Toxicology and Pharmaceutics-Drug Discovery
CiteScore
6.40
自引率
7.70%
发文量
80
审稿时长
41 days
期刊介绍: JARMAP is a peer reviewed and multidisciplinary communication platform, covering all aspects of the raw material supply chain of medicinal and aromatic plants. JARMAP aims to improve production of tailor made commodities by addressing the various requirements of manufacturers of herbal medicines, herbal teas, seasoning herbs, food and feed supplements and cosmetics. JARMAP covers research on genetic resources, breeding, wild-collection, domestication, propagation, cultivation, phytopathology and plant protection, mechanization, conservation, processing, quality assurance, analytics and economics. JARMAP publishes reviews, original research articles and short communications related to research.
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