Development of quantitative detection methods for four Alzheimer’s disease specific biomarker panels using electrochemical immunosensors based on enzyme immunoassay

IF 1.8 4区 化学 Q3 CHEMISTRY, ANALYTICAL Analytical Sciences Pub Date : 2024-06-17 DOI:10.1007/s44211-024-00614-7
Il Kyu Park, Young Sun Choi, Seo Yun Jo
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Abstract

Accurate and timely diagnosis of Alzheimer's disease (AD) is necessary to maximize the effectiveness of treatment and using biomarkers for diagnosis is attracting attention as a minimally invasive method with few side effects. Electrochemical immunosensor (EI) is a method that is in the spotlight in the medical and bioanalytical fields due to its portability and field usability. Here, we quantified four AD specific biomarkers using EIs based on enzyme immunoassay. We selected and developed quantitative methods for the biomarkers using screen-printed gold electrodes. For three biomarkers, quantification was performed using competition immunoassays in which antigen–antibody premix mixtures were applied to antigen-immobilized electrodes and the limit of detection (LOD) values were secured, 1.20 ng/ml, 1.30 ng/ml, and 1.74 ng/ml, respectively. For the other, a sandwich immunoassay using antibody pair was selected for quantification and LOD was also achieved as 0.077 ng/ml. All four biomarkers in buffer samples were successfully quantified and reliable R2 values were obtained, and reliable calibration curves were secured for three biomarkers in spiked human serum samples. The immunosensors developed and will be optimized are expected to be used in various fields, including detection of biomarkers for not only AD but also related diseases.

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利用基于酶免疫测定的电化学免疫传感器,开发四种阿尔茨海默病特异性生物标记物的定量检测方法。
为了最大限度地提高治疗效果,有必要对阿尔茨海默病(AD)进行准确而及时的诊断,而使用生物标记物进行诊断作为一种副作用小的微创方法正备受关注。电化学免疫传感器(EI)因其便携性和现场可用性而成为医学和生物分析领域的焦点。在这里,我们使用基于酶免疫测定的电化学免疫传感器对四种 AD 特异性生物标记物进行了定量分析。我们选择并开发了使用丝网印刷金电极的生物标记物定量方法。对于三种生物标记物,我们使用竞争免疫测定法进行了定量,在竞争免疫测定法中,抗原-抗体预混混合物被施加到抗原固定电极上,检测限(LOD)值分别为1.20纳克/毫升、1.30纳克/毫升和1.74纳克/毫升。至于另一种生物标记物,则选择了使用抗体对的夹心免疫测定法进行定量,检测限也达到了 0.077 纳克/毫升。所有四种生物标记物在缓冲液样品中的定量都获得了成功,并得到了可靠的 R2 值。已开发并将进行优化的免疫传感器有望应用于多个领域,包括检测注意力缺失症及相关疾病的生物标记物。
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来源期刊
Analytical Sciences
Analytical Sciences 化学-分析化学
CiteScore
2.90
自引率
18.80%
发文量
232
审稿时长
1 months
期刊介绍: Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.
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