Backbone and methyl side-chain resonance assignments of the Fab fragment of adalimumab

IF 0.8 4区 生物学 Q4 BIOPHYSICS Biomolecular NMR Assignments Pub Date : 2024-06-26 DOI:10.1007/s12104-024-10187-1
Muzaddid Sarker, Yves Aubin
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Abstract

Adalimumab is a therapeutic monoclonal antibody developed to target human TNF an important mediator of immune-mediated inflammatory diseases such as rheumatoid arthritis, amongst others. The 48 kDa Fab fragment of adalimumab was produced in Escherichia coli using a single chain approach to allow complete isotopic incorporation of deuterium, carbon-13 and nitrogen-15 along with the protonated isoleucine-d, valine and leucine methyl groups. Here we report the near complete resonance assignment of the polypeptide backbone and the methyl groups of isoleucine, leucine and valine residues.

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阿达木单抗 Fab 片段的骨架和甲基侧链共振分配。
阿达木单抗是一种治疗性单克隆抗体,针对人类 TNF 而开发,TNF 是类风湿性关节炎等免疫介导炎症性疾病的重要介质。阿达木单抗的 48 kDa Fab 片段是在大肠杆菌中使用单链方法生产的,这种方法可以将氘、碳-13 和氮-15 以及质子化的异亮氨酸-d、缬氨酸和亮氨酸甲基完全同位素化。在此,我们报告了多肽骨架以及异亮氨酸、亮氨酸和缬氨酸残基甲基的近乎完整的共振分配。
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来源期刊
Biomolecular NMR Assignments
Biomolecular NMR Assignments 生物-光谱学
CiteScore
1.70
自引率
11.10%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.
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