Alice Giusti , Lara Tinacci , Enrica Ricci , Francesco Verdigi , Roberto Narducci , Lisa Vallone , Laura Gasperetti , Andrea Armani
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引用次数: 0
Abstract
Multi-species mushroom-based products (MPs) sold in Italy were authenticated by metabarcoding (MB). One degenerated primer pair was projected for the ITS-1 region amplification. The final data were interpreted using positive controls and extraction blanks. Sequences of macrofungi, microfungi and bacteria were detected in 100%, 90.9% and 77.3% of the MPs, respectively. Not edible and toxic macrofungi were also found, although in low sequence amount. The match between species declared on MPs label and species detected by MB was 59.1%. Cases of voluntary species substitution cannot be excluded. Methods to authenticate these products, still poorly investigated, should be further employed, and MB protocol should be standardized to be used in the context of both official control and companies’ self-control. Alternative approaches should be considered for canned and frozen MPs, in virtue of the observed DNA fragmentation.
期刊介绍:
Food Control is an international journal that provides essential information for those involved in food safety and process control.
Food Control covers the below areas that relate to food process control or to food safety of human foods:
• Microbial food safety and antimicrobial systems
• Mycotoxins
• Hazard analysis, HACCP and food safety objectives
• Risk assessment, including microbial and chemical hazards
• Quality assurance
• Good manufacturing practices
• Food process systems design and control
• Food Packaging technology and materials in contact with foods
• Rapid methods of analysis and detection, including sensor technology
• Codes of practice, legislation and international harmonization
• Consumer issues
• Education, training and research needs.
The scope of Food Control is comprehensive and includes original research papers, authoritative reviews, short communications, comment articles that report on new developments in food control, and position papers.