Comparing Effects of Folic Acid, Epibrassinolide, Chitosan and Glutathione Foliar Treatments on Safflower’s Physiology and Yield during Water Stress

IF 1.1 4区 生物学 Q3 PLANT SCIENCES Russian Journal of Plant Physiology Pub Date : 2024-06-12 DOI:10.1134/s1021443723603312
N. Abdiazar, H. Zahedi, Y. Sharghi, S. A. M. Modarres-Sanavy, A. Alipour
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Abstract

While the potential for biostimulants to mitigate water stress holds promise, the underlying physiological mechanisms in safflower (Carthamus tinctorius L.) regarding this matterremain unclear. Elucidating how biostimulants treatments may induce adaptive changes in ROS scavenging, osmoregulation, carbon fixation, gas exchange, and provides insight into optimizing plant water stress tolerance at the cellular level. This split-plot study included biostimulant foliar treatments (folic acid, epibrassinolide, chitosan, glutathione) as subplots, and irrigation regimes (optimal, mild, severe water deficit) as main plots. Under mild water stress, folic acid and epibrassinolide sprays exhibited the highest increase in chlorophyll a (55.3%) and chlorophyll b (51.0%). These treatments also showed the most substantial increases in carotenoids (36.1%), relative water content (18.5%), and soluble proteins (20%). Proline levels were highest with glutathione and chitosan treatments, which increased levels by 81% compared to the control. Transpiration rates were 30% higher on average with folic acid, epibrassinolide, chitosan, and glutathione treatments. Folic acid led to the highest grain yield increase (26%) and GPX levels (22.9%). Severe water stress saw epibrassinolide and glutathione treatments increase relative water content the most (20%). Chitosan led to the highest increases in soluble proteins (23.2%) and transpiration (97%). Proline levels were 59.7% higher on average with folic acid, epibrassinolide, and chitosan treatments. GPX levels increased the most with epibrassinolide, chitosan, and glutathione (62%). Fv/Fm ratios were 170 and 50% higher with folic acid and chitosan or epibrassinolide and glutathione, respectively. Overall, folic acid and epibrassinolide performed best under mild stress, maintaining photosynthesis and yields through increasing chlorophyll, proteins, and water retention. Epibrassinolide, chitosan, and glutathione were most effective under severe stress, conferring drought tolerance by improving antioxidant defenses, protein levels, and water retention.

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比较叶酸、表黄素内酯、壳聚糖和谷胱甘肽叶面处理对红花在水分胁迫下的生理机能和产量的影响
摘要 虽然生物刺激素在缓解水分胁迫方面具有潜力,但红花(Carthamus tinctorius L.)在这方面的基本生理机制仍不清楚。阐明生物刺激素如何诱导 ROS 清除、渗透调节、碳固定、气体交换等方面的适应性变化,为在细胞水平上优化植物对水分胁迫的耐受性提供了见解。这项分小区研究将生物刺激剂叶面处理(叶酸、表阿霉素内酯、壳聚糖、谷胱甘肽)作为子小区,将灌溉制度(最佳、轻度、严重缺水)作为主小区。在轻度缺水胁迫下,叶酸和表巴戟内酯喷洒叶绿素 a(55.3%)和叶绿素 b(51.0%)的增幅最大。这些处理还显示类胡萝卜素(36.1%)、相对含水量(18.5%)和可溶性蛋白质(20%)的增幅最大。谷胱甘肽和壳聚糖处理的脯氨酸含量最高,比对照组提高了 81%。叶酸、表紫苏内酯、壳聚糖和谷胱甘肽处理的蒸腾速率平均提高 30%。叶酸使谷物产量增加最多(26%),GPX 水平增加最多(22.9%)。在严重的水分胁迫下,表紫苏内酯和谷胱甘肽处理的相对含水量增加最多(20%)。壳聚糖使可溶性蛋白质(23.2%)和蒸腾作用(97%)增加最多。叶酸、表黄素内酯和壳聚糖处理的脯氨酸水平平均提高了 59.7%。表布拉辛内酯、壳聚糖和谷胱甘肽的 GPX 含量增加最多(62%)。叶酸和壳聚糖或表巴素内酯和谷胱甘肽的 Fv/Fm 比率分别高出 170% 和 50%。总体而言,叶酸和表布拉辛内酯在轻度胁迫下表现最佳,它们通过增加叶绿素、蛋白质和保水性来维持光合作用和产量。在严重胁迫下,叶酸和表叶酸内酯的效果最好,它们通过提高抗氧化防御能力、蛋白质水平和保水性来提高耐旱性。
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来源期刊
CiteScore
4.00
自引率
14.30%
发文量
107
审稿时长
6 months
期刊介绍: Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.
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