Improved Growth and Metabolite Production in Chlorella sorokiniana under Mixotrophic Conditions with Acetate and Urea

IF 1.1 4区 生物学 Q3 PLANT SCIENCES Russian Journal of Plant Physiology Pub Date : 2024-07-02 DOI:10.1134/s1021443723603002
H. Mansouri, H. Salarinasab
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Abstract

In this study, changes in the growth and biochemical parameters of Chlorella sorokiniana under mixotrophic culture conditions with 50 mM acetate and acetate supplemented with 0.25, 0.50 and 1 mM of urea were investigated. Acetate increased fresh (FW) and dry (DW) weights by 54 and 40%, respectively, compared with the control. Acetate treatment also increased the amount of chlorophyll a and carotenoids by 2.93 and 3.32 times, respectively, compared with the control, but decreased the amount of chlorophyll b, protein, and soluble sugars. There was a significant increase in growth and in the amount of metabolites analyzed when urea was used together with acetate. The most suitable concentration of urea for increasing FW and DW, chl a and b, and protein was 1 mM, for increasing carotenoid 0.25 mM and for increasing soluble sugars 0.50 mM. The best results in the simultaneous treatment with acetate and urea for each of the parameters analyzed were as follows: carotenoid 5 times, FW 2.1 times, DW 2.5 times, chl a 3.35 times, chl b 2 times, protein 1.4 times and soluble sugar 1.3 times increase compared with the control. These results showed that the simultaneous use of a carbon source (acetate) and a nitrogen source (urea) was more effective in increasing growth and the amount of metabolites.

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在醋酸盐和尿素的混养条件下提高小球藻的生长和代谢产物产量
摘要 在本研究中,研究了在 50 mM 醋酸和醋酸盐辅以 0.25、0.50 和 1 mM 尿素的混养条件下,苏氏小球藻的生长和生化参数的变化。与对照组相比,醋酸盐分别增加了 54% 和 40% 的鲜重(FW)和干重(DW)。与对照相比,醋酸盐处理还使叶绿素 a 和类胡萝卜素的含量分别增加了 2.93 倍和 3.32 倍,但叶绿素 b、蛋白质和可溶性糖的含量却有所下降。当尿素与醋酸盐同时使用时,生长量和分析的代谢物数量都有明显增加。最适合增加净重和容重、叶绿素 a 和 b 以及蛋白质的尿素浓度为 1 毫摩尔,增加类胡萝卜素的浓度为 0.25 毫摩尔,增加可溶性糖的浓度为 0.50 毫摩尔。与对照组相比,同时使用醋酸盐和尿素处理各分析参数的最佳结果如下:类胡萝卜素增加 5 倍,FW 增加 2.1 倍,DW 增加 2.5 倍,chl a 增加 3.35 倍,chl b 增加 2 倍,蛋白质增加 1.4 倍,可溶性糖增加 1.3 倍。这些结果表明,同时使用碳源(醋酸盐)和氮源(尿素)能更有效地提高生长和代谢产物的数量。
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来源期刊
CiteScore
4.00
自引率
14.30%
发文量
107
审稿时长
6 months
期刊介绍: Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.
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