Lipoic Acid Can Maintain Stimulation of the Antioxidant System at Lower Reactive Oxygen Species, Ascorbate and Glutathione Levels in Osmotic Stressed Maize
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引用次数: 0
Abstract
Lipoic acid (LA), a unique antioxidant compound, can stimulate the antioxidant defense system in plants subjected to abiotic stresses. We aimed to determine the role of LA in induction of the antioxidant system at lower reactive oxygen species (ROS), ascorbate (ASC) and glutathione (GSH) levels in osmotic-stressed maize (Zea mays L.). For this purpose, ROS, GSH and ASC contents were decreased using N,N'‑dimethylthiourea (DMTU), L-buthionine sulfoximine (BSO) and acriflavine (AF), respectively. Pots containing 21-day-old seedlings were divided into nine groups consisting of a non-stressed group; polyethylene glycol6000 (PEG)-induced osmotic stress (PEG) group, LA, DMTU, BSO and AF treatment groups; and DMTU, BSO, and AF-combined LA treatment groups under osmotic stress. ROS contents and membrane damage after the DMTU, BSO, and AF-combined LA treatments were lower than those after the DMTU, BSO, and AF treatments, respectively. Moreover, the LA treatments in combination with DMTU, BSO and AF increased dry weight, activities of antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase and dehydroascorbate reductase), and contents of GSH, ASC, and LA compared to the DMTU, BSO and AF treatments. Additionally, the relative expression levels of SUPEROXIDE DISMUTASE, CATALASE1 and ASCORBATE PEROXIDASE1 genes were consistent with the findings for their related antioxidant enzyme activities. These results indicated that LA could adjust ROS level and maintain stimulation of the antioxidant system at lower ROS, GSH, and ASC levels in osmotic stressed maize. Furthermore, LA may play a signaling role and assume the function of ASC and GSH in maize under PEG-induced osmotic stress.
期刊介绍：
Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.
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