{"title":"Hormetic Effects of Low Dose Gamma Irradiation on Antioxidant Defense System and Thymol Biosynthesis in Thyme Plants","authors":"M. Norouzi, F. Sanjarian, S. Shahbazi","doi":"10.1134/s1021443724603914","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>This study investigated the impact of different doses (0, 1, 3, and 5 Gy) of gamma radiation on antioxidant mechanisms and thymol biosynthesis in <i>Thymus vulgaris</i>. Concentration of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and malondialdehyde (MDA) exhibited dose-dependent increases, indicating oxidative damage and a time-dependent progression of stress. Superoxide dismutase (SOD) activities were triggered in response, accompanied by time-dependent variations in peroxidase (POD) and polyphenol oxidase (PPO) activities. Biochemical analyses revealed enhanced total phenolic content (TPC), total flavonoid content (TFC) and anthocyanin levels at 1 Gy. Additionally, the effect of radiation on the expression of pivotal genes in the biosynthesis pathway of thymol, such as <i>DXR, TvTPS</i>, and <i>CYP71D178</i>, was investigated. Gamma irradiation significantly up-regulated <i>DXR</i> transcription at higher doses, while <i>TvTPS</i> and <i>CYP71D178</i> transcription peaked at 1 Gy. Thymol emerged as the predominant compound in the essential oil composition, experiencing a significant increase at 1 Gy, thus illustrating a hormetic response. This study provides scientific insights into the hormesis effects of gamma irradiation on antioxidant responses and thymol biosynthesis in <i>Thymus vulgaris</i>, contributing to a better understanding of the complicated biochemical processes involved in plant adaptation to radiation stress.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Russian Journal of Plant Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s1021443724603914","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
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Abstract
This study investigated the impact of different doses (0, 1, 3, and 5 Gy) of gamma radiation on antioxidant mechanisms and thymol biosynthesis in Thymus vulgaris. Concentration of hydrogen peroxide (H2O2) and malondialdehyde (MDA) exhibited dose-dependent increases, indicating oxidative damage and a time-dependent progression of stress. Superoxide dismutase (SOD) activities were triggered in response, accompanied by time-dependent variations in peroxidase (POD) and polyphenol oxidase (PPO) activities. Biochemical analyses revealed enhanced total phenolic content (TPC), total flavonoid content (TFC) and anthocyanin levels at 1 Gy. Additionally, the effect of radiation on the expression of pivotal genes in the biosynthesis pathway of thymol, such as DXR, TvTPS, and CYP71D178, was investigated. Gamma irradiation significantly up-regulated DXR transcription at higher doses, while TvTPS and CYP71D178 transcription peaked at 1 Gy. Thymol emerged as the predominant compound in the essential oil composition, experiencing a significant increase at 1 Gy, thus illustrating a hormetic response. This study provides scientific insights into the hormesis effects of gamma irradiation on antioxidant responses and thymol biosynthesis in Thymus vulgaris, contributing to a better understanding of the complicated biochemical processes involved in plant adaptation to radiation stress.
期刊介绍：
Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.
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