Influence of Jasmonates, Salicylic Acid and 24-Epibrassinolide on Other Plant Growth Regulators during Development and Germination in the Recalcitrant Seeds of Artocarpus hirsutus Lam.

IF 1.1 4区生物学 Q3 PLANT SCIENCES Russian Journal of Plant Physiology Pub Date : 2024-06-12 DOI:10.1134/s1021443724603987

S. Sreedevi, K. G. Ajith Kumar, V. V. Amritha, Sneha John

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Abstract

Artocarpus hirsutus Lam. is a tropical, endemic, keystone tree species of the Western Ghats commercially utilized for its valuable timber. The seeds are reported to be recalcitrant, but its exact physiology and the hormonal changes during development and germination has not been well studied. The present study was intended to understand the hormonal dynamics and their interaction to get an idea about the up and down regulation during seed development and germination. Fifteen hormones were simultaneously analyzed using LC MS/MS technique. Our results showed that during histodifferentiation IAA, GA₄, cytokinins (tZ and tZR), JA and 24-epibrassinolide (24-epiBL) were found in higher levels, but during seed expansion all these plant hormones began to decrease. However salicylic acid and cis jasmone were found elevated during germination. Like other recalcitrant seeds ABA was found in higher level during early embryogeny but declined sharply in the later embryogeny and germination. JA showed strong positive correlation with IAA (0.997**), ABA (0.955*), tZ (0.997**) and tZR (0.975*) possibly acts as an inducer of all these hormones, but no significant relationship with other hormones. However SA, which was found in extremely higher level during development and germination, did not show any significant relationship with other hormones, possibly functioning in defense mechanism. 24-epiBL was found in significant level, but showed insignificant relationship with hormones. Natural desiccation had a negative impact on the synthesis/accumulation of PGRs. Electrical conductivity studies revealed that cell membrane damage of the embryonic tissue was responsible for the loss of viability of these recalcitrant seeds.

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茉莉酮酸盐、水杨酸和 24-表紫草素内酯在芒柄蒿种子发育和发芽过程中对其他植物生长调节剂的影响

摘要Artocarpus hirsutus Lam.是西高止山脉的一种热带特有的基石树种，因其珍贵的木材而被商业利用。据报道，其种子具有抗逆性，但其确切的生理机能以及发育和萌发过程中的激素变化尚未得到很好的研究。本研究旨在了解激素的动态变化及其相互作用，从而了解种子发育和萌发过程中的上下调节。采用 LC MS/MS 技术同时分析了 15 种激素。结果表明，在组培过程中，IAA、GA4、细胞分裂素（tZ 和 tZR）、JA 和 24-eprassinolide (24-epiBL) 的含量较高，但在种子膨大过程中，所有这些植物激素的含量都开始下降。不过，水杨酸和顺式茉莉酮在萌发过程中被发现升高了。与其他顽劣种子一样，ABA 在早期胚胎发育过程中含量较高，但在后期胚胎发育和萌发过程中急剧下降。JA 与 IAA（0.997**）、ABA（0.955*）、tZ（0.997**）和 tZR（0.975*）呈很强的正相关，可能是所有这些激素的诱导剂，但与其他激素没有显著关系。然而，在发育和发芽过程中含量极高的 SA 与其他激素没有明显的关系，可能起着防御机制的作用。24-epiBL 的含量很高，但与激素的关系不大。自然干燥对 PGRs 的合成/积累有负面影响。电导率研究表明，胚组织的细胞膜损伤是这些顽固种子丧失活力的原因。

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来源期刊

Russian Journal of Plant Physiology 生物-植物科学

CiteScore

4.00

自引率

14.30%

发文量

107

审稿时长

6 months

期刊介绍： Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.