Paulo H. G. A. de Oliveira, Ana C. O. Barbosa, Nathiele S. Araújo, Marcio G. C. Costa
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引用次数: 0
Abstract
DNA methylation balance plays an important regulatory role in drought response and tolerance and also in the control of tomato fruit ripening, and its study may help to understand how fleshy fruits respond to drought. This work aimed to investigate how drought affects the expression profile of genes related to DNA methylation balance during tomato fruit ripening. Micro-Tom tomato plants were subjected to irrigated control (C) and two water deficit intensities (T1 and T2) treatments and analyzed for leaf gas exchange rates, leaf water potential (Ψw) and expression of genes encoding DNA methylases/demethylases during the mature green (MG), breaker (BR) and red ripe (RR) stages of fruit ripening. Water deficit promoted a significant reduction in the values of Ψw, photosynthetic rate (A), transpiration (E), stomatal conductance (gs), regardless of its applied intensity. In silico analysis of RNA-Seq data showed that CHROMOMETHYLASE 2 (SlCMT2) and DEMETER-LIKE 2 (SlDML2) were those, among all tomato DNA methylases/demethylases genes, that showed dramatic and opposite changes in expression during fruit ripening. Water deficit positively regulated the expression of SlCMT2 in the MG stage and SlDML2 in BR, with no significant variations in expression for these genes in the other stages of fruit ripening. These results show that genes related to DNA methylation balance have their expression further increased by water deficit in the fruit ripening stages in which they act, suggesting that drought enhances, but does not disrupt, the intrinsically programmed DNA methylation dynamics necessary for ripening progression.
DNA 甲基化平衡在干旱响应和耐旱性以及番茄果实成熟调控中起着重要的调控作用，对其研究可能有助于了解肉质果实如何响应干旱。本研究旨在探讨干旱如何影响番茄果实成熟过程中 DNA 甲基化平衡相关基因的表达谱。将微型番茄植株置于灌溉对照（C）和两种水分亏缺强度（T1和T2）处理下，分析了果实成熟期（MG）、破碎期（BR）和红熟期（RR）的叶片气体交换速率、叶片水势（Ψw）和编码DNA甲基化酶/脱甲基酶基因的表达。水分亏缺导致Ψw、光合速率（A）、蒸腾（E）、气孔导度（gs）的值明显下降，而与施加的强度无关。对 RNA-Seq 数据的硅学分析表明，在所有番茄 DNA 甲基化酶/脱甲基酶基因中，CHROMOMETHYLASE 2（SlCMT2）和 DEMETER-LIKE 2（SlDML2）在果实成熟过程中的表达量出现了巨大的相反变化。水分亏缺正向调节 MG 阶段 SlCMT2 和 BR 阶段 SlDML2 的表达，而在果实成熟的其他阶段，这些基因的表达没有显著变化。这些结果表明，与 DNA 甲基化平衡有关的基因在其作用的果实成熟阶段会因缺水而进一步提高表达量，这表明干旱会增强但不会破坏成熟过程中所需的内在程序化 DNA 甲基化动态。
期刊介绍：
The journal does not publish articles in taxonomy, anatomy, systematics and ecology unless they have a physiological approach related to the following sections:
Biochemical Processes: primary and secondary metabolism, and biochemistry;
Photobiology and Photosynthesis Processes;
Cell Biology;
Genes and Development;
Plant Molecular Biology;
Signaling and Response;
Plant Nutrition;
Growth and Differentiation: seed physiology, hormonal physiology and photomorphogenesis;
Post-Harvest Physiology;
Ecophysiology/Crop Physiology and Stress Physiology;
Applied Plant Ecology;
Plant-Microbe and Plant-Insect Interactions;
Instrumentation in Plant Physiology;
Education in Plant Physiology.
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