Preliminary Analysis of Potentially Overlapping Differentially Expressed Proteins in Both the Spinal Cord and Brain of SOD1 G93A Mice.

IF 1.9 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Current protein & peptide science Pub Date : 2024-07-09 DOI:10.2174/0113892037293525240621120033

Shishi Jiang, Hongbing Nie, Shan Hua, Meng Xie, Renshi Xu

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Abstract

Objective: Proteomic elucidation is an essential step in improving our understanding of the biological properties of proteins in amyotrophic lateral sclerosis (ALS).

Methods: Preliminary proteomic analysis was performed on the spinal cord and brain of SOD1 G93A (TG) and wild-type (WT) mice using isobaric tags for relative and absolute quantitation.

Results: Partial up- and downregulated proteins showing significant differences between TG and WT mice were identified, of which 105 proteins overlapped with differentially expressed proteins in both the spinal cord and brain of progression mice. Bioinformatic analyses using Gene Ontology, a cluster of orthologous groups, and Kyoto Encyclopedia of Genes and Genomes pathway revealed that the significantly up- and downregulated proteins represented multiple biological functions closely related to ALS, with 105 overlapping differentially expressed proteins in the spinal cord and brain at the progression stage of TG mice closely related to 122 pathways. Differentially expressed proteins involved in a set of molecular functions play essential roles in maintaining neural cell survival.

Conclusion: This study provides additional proteomic profiles of TG mice, including potential overlapping proteins in both the spinal cord and brain that participate in pathogenesis, as well as novel insights into the up- and downregulation of proteins involved in the pathogenesis of ALS.

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对 SOD1 G93A 小鼠脊髓和大脑中潜在重叠差异表达蛋白的初步分析

目的蛋白质组学分析是提高我们对肌萎缩性脊髓侧索硬化症（ALS）蛋白质生物学特性认识的重要一步：使用等位标签对 SOD1 G93A（TG）小鼠和野生型（WT）小鼠的脊髓和大脑进行了初步的蛋白质组分析，以进行相对和绝对定量：结果：发现了TG和WT小鼠之间存在显著差异的部分上调和下调蛋白，其中105个蛋白与进展期小鼠脊髓和大脑中的差异表达蛋白重叠。利用基因本体、同源群和京都基因组百科全书途径进行的生物信息学分析表明，显著上调和下调的蛋白质代表了与ALS密切相关的多种生物学功能，其中105个重叠的差异表达蛋白质在TG小鼠进展期的脊髓和大脑中与122种途径密切相关。参与一系列分子功能的差异表达蛋白在维持神经细胞存活方面发挥着至关重要的作用：本研究提供了更多 TG 小鼠的蛋白质组图谱，包括脊髓和大脑中参与发病机制的潜在重叠蛋白质，以及对参与 ALS 发病机制的蛋白质上调和下调的新见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current protein & peptide science 生物-生化与分子生物学

CiteScore

5.20

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Current Protein & Peptide Science publishes full-length/mini review articles on specific aspects involving proteins, peptides, and interactions between the enzymes, the binding interactions of hormones and their receptors; the properties of transcription factors and other molecules that regulate gene expression; the reactions leading to the immune response; the process of signal transduction; the structure and function of proteins involved in the cytoskeleton and molecular motors; the properties of membrane channels and transporters; and the generation and storage of metabolic energy. In addition, reviews of experimental studies of protein folding and design are given special emphasis. Manuscripts submitted to Current Protein and Peptide Science should cover a field by discussing research from the leading laboratories in a field and should pose questions for future studies. Original papers, research articles and letter articles/short communications are not considered for publication in Current Protein & Peptide Science.