H. Cao, A. Ghorbanpour, A. Ghorbani, M. Zargar, S. M. Razavi
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引用次数: 0
Abstract
Arsenic (As), a hazardous metal, is widely found in industrial and agricultural wastewater, as well as in pesticides. Silicon (Si) can be crucial in mitigating As toxicity. This research aimed to investigate 0.5 and 1 mM Si treatments on Calendulaofficinalis under As toxicity (25 and 50 µM). As treatments significantly reduced growth characteristics, photosynthesis (Fv/Fm and photosynthetic pigments), and the accumulation of some essential elements (N, Ca, P, Zn, K, Mn, and Fe), while increasing the levels of H2O2, \({\text{O}}_{2}^{ - }\), and MG. Contrarily, the external application of Si to C. officinalis L. restricted As accumulation, leading to less oxidative damage and better accumulation of elements and photosynthesis. Additionally, the levels of sulfur-containing compounds (GSH, NPTs, PCs, and cysteine) increased, along with enhanced Gly I and Gly II activities and antioxidant activities (CAT, SOD, GR, and APX), leading to a notable drop in free toxic radicals and lipid peroxidation in As-stressed plants. Furthermore, Si supplementation regulated the thiol metabolism enzyme glutathione-S-transferase and improved cysteine synthesis by increasing the activities of serine acetyltransferase, cysteine synthase (CS), and adenylyl sulfate reductase. Therefore, this study showcases the significant contribution of Si in promoting growth, facilitating photosynthesis, and fostering As tolerance through various pathways, such as enhancing antioxidant activities, the glyoxalase system, non-enzymatic and enzymatic sulfur metabolism, reducing As uptake, and increasing the accumulation of essential elements.
摘要砷（As）是一种有害金属，广泛存在于工业和农业废水以及农药中。硅（Si）对减轻砷的毒性至关重要。本研究旨在调查 0.5 和 1 mM 硅处理对金盏花在砷毒性（25 和 50 µM）条件下的影响。As处理明显降低了金盏花的生长特性、光合作用（Fv/Fm和光合色素）以及一些必需元素（N、Ca、P、Zn、K、Mn和Fe）的积累，同时增加了H2O2、\({\text{O}}_{2}^{ - }\) 和MG的水平。相反，外部施用 Si 限制了 As 的积累，从而减少了氧化损伤，提高了元素积累和光合作用。此外，含硫化合物（GSH、NPTs、PCs 和半胱氨酸）的含量增加了，Gly I 和 Gly II 的活性以及抗氧化活性（CAT、SOD、GR 和 APX）也增强了，从而导致 As 胁迫植物体内的自由毒性自由基和脂质过氧化显著下降。此外，补充 Si 还能调节硫醇代谢酶谷胱甘肽-S-转移酶，并通过提高丝氨酸乙酰转移酶、半胱氨酸合成酶（CS）和腺苷酸硫酸还原酶的活性来改善半胱氨酸的合成。因此，本研究通过提高抗氧化活性、乙醛化酶系统、非酶和酶硫代谢、减少砷吸收和增加必需元素积累等多种途径，展示了 Si 在促进生长、促进光合作用和提高砷耐受性方面的重要作用。
期刊介绍：
Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.
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