Waqas Ashraf, Khubaib Ali, Hafiz Rizwan Sharif, Arif Hussain, Aiman Karim, Anam Latif, Muhammad Umair Khalid, Zhang Lianfu
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引用次数: 0
Abstract
In the present study, the fenugreek seed extract was loaded into chitosan-coated nanoliposomes (FCNL) to enhance the stability and preserve the bioactivity of extracted flavonoids. To accomplish this, the preparation and optimization results showed that 500-W ultrasonic power, 1.5% extract loading, and 0.5% chitosan coating produced stable FCNL with an average particle size of 178.7 nm, PDI (0.20), and zeta potential of + 15.9 mV with the highest encapsulation efficiency of 86.18%. The structural and thermal characterization by FTIR, XRD, and TGA suggested strong electrostatic interaction and enhanced thermal stability of FCNL as compared to FWNL. The morphological study indicated spherical nanoparticles with smooth surfaces. The results of storage stability revealed that the prepared nanoparticles were sensitive to light and could be effectively stored at low temperatures (4 °C) in dark conditions for a longer period of time with maximum flavonoid retention of 96.27%. The prepared FCNL showed significant antibacterial and free radical (DPPH and ABTS) scavenging activities. The results of the in vitro release profile revealed that the FCNL was stable during gastric digestion with only an 11.31% release rate which enhanced to a highest of 79.67% in simulated intestinal fluid (SIF) after 6 h of digestion. Subsequently, the FCNL presented notable inhibition activities against α-amylase (76.89 ± 2.07%), α-glucosidase (89.35 ± 1.87%), and pancreatic lipase (74.22 ± 2.05%). Hence, the bioactivity, stability, and release profile of fenugreek flavonoids can be effectively preserved using nanoliposomes as carrier agents. Conclusively, the prepared nanoparticles can be used in food and pharmaceutical industries as natural antidiabetic and hypolipidemic agents.
期刊介绍:
Food and Bioprocess Technology provides an effective and timely platform for cutting-edge high quality original papers in the engineering and science of all types of food processing technologies, from the original food supply source to the consumer’s dinner table. It aims to be a leading international journal for the multidisciplinary agri-food research community.
The journal focuses especially on experimental or theoretical research findings that have the potential for helping the agri-food industry to improve process efficiency, enhance product quality and, extend shelf-life of fresh and processed agri-food products. The editors present critical reviews on new perspectives to established processes, innovative and emerging technologies, and trends and future research in food and bioproducts processing. The journal also publishes short communications for rapidly disseminating preliminary results, letters to the Editor on recent developments and controversy, and book reviews.