Evolution of glucuronoxylan side chain variability in vascular plants and the compensatory adaptations of cell wall–degrading hydrolases

IF 8.1 1区 生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2024-07-12 DOI:10.1111/nph.19957
Li Yu, Louis F. L. Wilson, Oliver M. Terrett, Joel Wurman-Rodrich, Jan J. Łyczakowski, Xiaolan Yu, Kristian B. R. M. Krogh, Paul Dupree
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维管束植物葡萄糖醛酸侧链变异的进化和细胞壁降解水解酶的补偿性适应。
多糖结构的复杂性不仅会影响细胞壁的强度和延展性,还会阻碍病原体和生物技术对细胞壁进行糖化的尝试。在某些物种和组织中,木聚糖上的葡萄糖醛酸侧基呈现阿拉伯吡喃糖或半乳糖装饰,其遗传和进化基础完全未知,这阻碍了了解其功能和设计细胞壁消化率的努力。通过遗传学和多糖分析,我们从提出的候选基因中确定了拟南芥和桉树的责任基因座,而系统进化则发现了共同的进化起源。通过电泳分析了 GH30 家族内切-葡萄糖醛酸氧聚糖酶的活性,并通过系统发育和结构分析合理解释了它们不同的特异性。新发现的木聚糖阿拉伯吡喃糖基转移酶是高尔基体糖基转移酶 GT47-A 家族中一个被忽视的亚家族,该亚家族以前被认为主要由木聚糖半乳糖基转移酶组成。进一步的新功能化产生了一种桃金娘科特异性木聚糖半乳糖基转移酶。与此同时,GH30 内切葡萄糖醛酸氧聚糖酶也趋同地适应了克服这些装饰的能力,这表明这些结构在防御中的作用。然而,不同桉树组织中葡萄糖醛酸聚糖修饰基因的不同表达暗示了更多的功能。我们的研究结果表明了生物合成和降解碳水化合物活性酶活动的快速适应性,有助于深入了解植物与病原体之间的相互作用,促进植物细胞壁生物技术的利用。
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New Phytologist
New Phytologist 生物-植物科学
自引率
5.30%
发文量
728
期刊介绍: New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.
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