V. M. Alexandrova, G. R. Gumerova, Kh. G. Musin, Z. A. Berezhneva, A. A. Galimova, B. R. Kuluev
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引用次数: 0
Abstract
When cultivating calli generated from mature germs of spring bread wheat (Triticum aestivum L.), variety Fisht, spontaneous regeneration of chlorophyll-containing green roots sometimes occurred. When isolated from callus, these roots were capable of unlimited growth on hormone-free nutrient media, similar to cultures of hairy roots of dicotyledonous plants. Ordinary wheat roots did not grow under these conditions, while green roots continued to grow even when exposed to 100 mM NaCl. Typical chloroplasts were found in the parenchyma cells of green wheat roots. Approximately equal amounts of chlorophyll a and b were found in green wheat roots; however, their total content was tens of times less than in the leaves. Green roots were characterized by a higher proline content and higher activity of superoxide dismutase, catalase, and peroxidase than ordinary wheat roots, both under normal and under salinity conditions. Green roots may provide an alternative to hairy roots, which are very difficult to generate from grasses and other monocots, for use in both basic research and applied applications.
摘要 在培养由春面包小麦（Triticum aestivum L.）品种 Fisht 的成熟胚芽产生的胼胝体时，有时会出现自发再生的含叶绿素的绿色根。从胼胝体中分离出来后，这些根能够在不含激素的营养培养基上无限生长，类似于双子叶植物的毛根培养物。在这些条件下，普通小麦根不会生长，而绿色根即使暴露在 100 mM NaCl 中也能继续生长。在绿色小麦根的实质细胞中发现了典型的叶绿体。绿色小麦根中叶绿素 a 和叶绿素 b 的含量大致相同，但总含量比叶片少几十倍。与普通小麦根相比，绿色根的脯氨酸含量更高，超氧化物歧化酶、过氧化氢酶和过氧化物酶的活性也更高，无论是在正常条件下还是在盐度条件下都是如此。在基础研究和应用方面，绿根可以替代很难从禾本科和其他单子叶植物中生成的毛根。
期刊介绍：
Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.
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