Assessment of cell cycle arrest biomarkers and neutrophil gelatinase-associated lipocalin to distinguish acute kidney injury from other diseases in dogs

IF 2.1 2区农林科学 Q1 VETERINARY SCIENCES Journal of Veterinary Internal Medicine Pub Date : 2024-07-16 DOI:10.1111/jvim.17143

Ann Biscop, Donatienne Castelain, Emmelie Stock, Kristel Demeyere, Evelyne Meyer, Nausikaa Devriendt, Elisabeth Dorn, Nikita De Laet, Dominique Paepe

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Abstract

Background

Cell cycle arrest biomarkers (tissue inhibitor of metalloproteinase-2 [uTIMP-2] and insulin-like growth factor binding protein 7 [uIGFBP7]), and neutrophil gelatinase-associated lipocalin (NGAL) variables are valuable biomarkers for early diagnosis of acute kidney injury (AKI) in people.

Objectives

To evaluate uTIMP-2, uIGFBP7, fractional excretion of NGAL (FeNGAL), and urinary to serum NGAL ratio (u/sNGAL) in healthy dogs, dogs with AKI, dogs with chronic kidney disease (CKD), and critically ill (CI) dogs.

Animals

Forty-two client-owned dogs (healthy, n = 10; AKI, n = 11; CKD, n = 11; CI, n = 10).

Methods

Prospective, observational study. After assessment of routine renal biomarkers, stress (uTIMP-2, uIGFBP7) and damage (NGAL) biomarkers were measured, using ELISA kits, and normalized to urinary creatinine (uCr).

Results

Normalized uTIMP-2 and [uTIMP-2] × [uIGFBP7]/uCr were significantly higher in the AKI group (median 151.9 [range, 2.2-534.2] and 62.9 [1.1-266.8] pg/mL respectively), compared to healthy dogs (0.3 [0.2-74.7]; P < .001 and 0.16 [0.1-58.1] pg/mL; P < .001), dogs with CKD (0.7 [0.3-742.5]; P = .04 and 0.37 [0.2-180.1] pg/mL; P = .03) and CI dogs (1.9 [0.2-37.0]; P = .03 and 0.8 [0.1-16.1] pg/mL; P = .02). Fractional excretion of NGAL was significantly higher in dogs with AKI (54.17 [7.93-155.32] %), than in healthy (0.03 [0.01-0.21] %; P < .001) and CI dogs (3.05 [0.05-28.86] %; P = .02).

Conclusions and Clinical Importance

Normalized uTIMP-2, [uTIMP-2] × [uIGFBP7]/uCr, and FeNGAL can be valuable renal biomarkers for early diagnosis of AKI in dogs.

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评估细胞周期停滞生物标志物和中性粒细胞明胶酶相关脂质体，以区分犬急性肾损伤和其他疾病。

背景：细胞周期停滞生物标志物（金属蛋白酶组织抑制剂-2 [uTIMP-2]和胰岛素样生长因子结合蛋白7 [uIGFBP7]）和中性粒细胞明胶酶相关脂质体（NGAL）变量是早期诊断急性肾损伤（AKI）的重要生物标志物：目的：评估健康狗、AKI 狗、慢性肾脏病（CKD）狗和重症（CI）狗的uTIMP-2、uIGFBP7、NGAL 的部分排泄量（FeNGAL）和尿液与血清 NGAL 比值（u/sNGAL）：42只客户饲养的狗（健康，n = 10；AKI，n = 11；CKD，n = 11；CI，n = 10）：方法：前瞻性观察研究。在评估常规肾脏生物标志物后，使用 ELISA 试剂盒测量应激（uTIMP-2、uIGFBP7）和损伤（NGAL）生物标志物，并与尿肌酐（uCr）进行归一化：结果：与健康犬（0.3 [0.2-74.7]; P）相比，AKI 组归一化 uTIMP-2 和 [uTIMP-2] × [uIGFBP7]/uCr 明显更高（中位数分别为 151.9 [2.2-534.2] 和 62.9 [1.1-266.8] pg/mL）：归一化uTIMP-2、[uTIMP-2] × [uIGFBP7]/uCr和FeNGAL是早期诊断犬AKI的重要肾脏生物标志物。

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来源期刊

Journal of Veterinary Internal Medicine 农林科学-兽医学

CiteScore

4.50

自引率

11.50%

发文量

243

审稿时长

22 weeks

期刊介绍： The mission of the Journal of Veterinary Internal Medicine is to advance veterinary medical knowledge and improve the lives of animals by publication of authoritative scientific articles of animal diseases.