Journal of Veterinary Internal Medicine最新文献

Corticosteroid Administration Enhances the Glycemic, Insulinemic, and Incretin Responses to a High-Protein Mixed Meal in Adult Horses

IF 2.1 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine

Pub Date : 2025-03-10 DOI: 10.1111/jvim.17305

Allison T. Palmer, Mauria R. Watts, Kathryn J. Timko, Erin F. Pinnell, Katelyn A. Keefer, Olivia Gorman, Laura D. Hostnik, Teresa A. Burns

Background

Corticosteroids are used routinely in horses and induce insulin dysregulation (ID). Nutrition is important for ID management and includes low nonstructural carbohydrate (NSC) diets and, often, high-protein ration balancers (RB). Insulin and incretin secretion increase after high-protein meals; corticosteroids may influence these effects.

Hypothesis

A high-protein mixed meal will induce hyperinsulinemia and increased concentrations of glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide 1 (GLP-1) in horses with ID; dexamethasone (DEX) will amplify this effect.

Animals

Five horses with naturally occurring ID.

Methods

Horses underwent an IV glucose tolerance test and a feed challenge test (FCT; 1 kg RB). Tests were repeated after DEX administration (0.08 mg/kg PO q24h, 7 days). Insulin, glucose, and incretin dynamics were compared pre- and post-DEX.

Results

Corticosteroids exacerbated post-prandial hyperinsulinemia and hyperglycemia after a high-protein meal. The FCT area under the curve for insulin (AUC_INS) after DEX was significantly higher than baseline (558 ± 182 μIU/mL × min vs. 257 ± 93.9 μIU/mL × min; p = 0.03). The maximum concentration of GIP (C_maxGIP) after DEX (381 ± 70.6 pg/mL) was significantly higher than baseline (262 ± 13.7 pg/mL; p = 0.013). The AUC for GLP-1 (AUC_GLP-1; 31.1 ± 15.2 vs. 50 ± 20.2 pg/mL; p = 0.19) and the C_max of GLP-1 (C_maxGLP-1; 39.1 ± 25.3 vs. 29.6 ± 12.2 pg/mL; p = 0.32) did not differ between DEX and baseline.

Conclusions and Clinical Importance

Metabolic responses to a high-protein mixed meal were exacerbated by corticosteroids. Horses receiving corticosteroids had larger GIP responses, which may enhance post-prandial hyperinsulinemia.

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引用次数: 0

Factors Affecting the Quality of Histopathologic Specimens Obtained via Small Intestinal Endoscopic Biopsy in Dogs and Cats

IF 2.1 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine

Pub Date : 2025-03-08 DOI: 10.1111/jvim.70059

Ko Nakashima, Kazuhiro Kojima, Yoshinori Takeuchi, Manami Ito, Isao Matsumoto, Takahiro Ushigusa, Hiroshi Ohta, Kazuyuki Uchida

Background

The factors affecting the quality of histopathologic specimens obtained via small intestinal endoscopic biopsy (SIEB) remain unclear.

Hypothesis/Objectives

To identify factors related to the quality of histopathologic specimens obtained via SIEB.

Animals

Histopathologic duodenal and ileal specimens were obtained from 116 dogs and 38 cats that underwent SIEB for diagnostic purposes.

Methods

This retrospective study analyzed 3354 individual histopathologic specimens scored using the grading system of histopathologic adequacy (GSHA). A lower GSHA score indicates lower quality specimens. Univariate and multivariate ordinal logistic models were used to assess the relationship between the GSHA score of the specimens and various explanatory factors, including fixation method, biopsy forceps size, biopsy site, and histopathologic diagnosis. The generalized estimating equation method was used to account for the clustering of specimens among animals.

Results

Multivariate models using the specimens showed that filter paper fixation of endoscopic samples resulted in a higher GSHA score than floating fixation in both dogs (ordinal odds ratio [OR]: 0.19; 95% confidence interval [CI]: 0.15–0.25) and cats (ordinal OR: 0.19; 95% CI: 0.13–0.29). In dogs, the scores were lower for duodenal specimens than for ileal specimens and for specimens obtained using smaller forceps. In cats, the scores were lower for ileal specimens than for duodenal specimens and for older animals.

Conclusion and Clinical Importance

The quality of histopathologic specimens obtained via SIEB is influenced by the fixation method. Additionally, other factors differ between dogs and cats. These results contribute to improved SIEB practices in veterinary medicine.

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引用次数: 0

Daily Heart Rate Variability in Dogs With Atrial Fibrillation

IF 2.1 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine

Pub Date : 2025-03-08 DOI: 10.1111/jvim.70051

Joao Escalda, Brigite Pedro, Jose Novo Matos, Antonia Mavropoulou, Christopher Linney, Joao Neves, Joanna Dukes-McEwan, Anna R. Gelzer

Background

Daily variability of heart rate in 24-h Holter recordings in dogs with atrial fibrillation (AF) receiving antiarrhythmic drugs (AAD) is unknown and could influence medical decisions.

Hypothesis/Objectives

Dogs with AF, Holter-derived mean heart rate (meanHRHolter) over 24 h is not significantly different from a subsequent, consecutive 24-h period.

Animals

Twenty-five dogs with AF.

Methods

Prospective, descriptive, multicenter study. MeanHRHolter rate and ventricular arrhythmias (VAs) were prospectively analyzed after starting AAD. Clinically relevant difference (defined as ≥ 10 bpm in the meanHRHolter), success of rate control (defined as meanHRHolter ≤ 125 bpm). A Bland–Altman analysis and intra-class correlation coefficient (ICC) were calculated to compare two consecutive 24-h Holter recordings. VAs percentage difference [(maximum daily value-minimum daily value)/maximum daily value × 100] and grading variability between recordings were also investigated.

Results

Small BIAS with ICC 0.98 (95% confidence interval [CI] 0.95–0.99) on meanHRHolter with no statistical difference between two consecutive 24-h Holter recordings (95% CI [−2.84–2.92], degree of freedom 24, p = 0.98). Only 2/25 dogs (8%; 95% CI [2%–25%]) had clinically significant variation, while 1/25 (4%; 95% CI [0%–20%]) dogs showed different classifications in the success of rate control between the consecutive recordings. The VAs percentage difference was 52%, with 7/25 (28%; 95% CI [14%–47%]) dogs showing a VAs grading difference of ≥ 2.

Conclusion and Clinical Importance

The daily heart rate variability in dogs with AF receiving AAD is low, suggesting that a single 24-h Holter recording is adequate to assess rate control. Daily variability might be an important consideration when assessing VAs in dogs with concomitant AF.

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引用次数: 0

Blood Carboxyhemoglobin Concentrations as a Diagnostic Biomarker of Hemolytic Anemias in Cats

IF 2.1 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine

Pub Date : 2025-03-08 DOI: 10.1111/jvim.70058

Ran Nivy, Gila Abells Sutton, Yaron Bruchim

Background

Endogenous carboxyhemoglobin (COHb) production is a byproduct of hemoglobin metabolism.

Hypothesis

Blood carboxyhemoglobin concentrations are higher in cats with hemolytic anemia (HA).

Animals

Twenty cats with HA, 29 cats with non-HA, and 22 controls were prospectively followed.

Methods

Blood tests were performed upon admission. The Mann–Whitney and Kruskal–Wallis tests were used for comparisons. Receiver-operating characteristic (ROC) analyses tested COHb as a marker of HA or survival.

Results

The HA group included 17 cats with immune-mediated HA and 3 with Heinz body (HB) anemia. In the non-HA group, leading diagnoses included kidney disease (n = 14), acute/chronic blood loss (n = 11) and pancytopenia (n = 3). Carboxyhemoglobin concentrations (median [IQR]) significantly differed between cats with HA (5.55% [1.9]) and cats with non-HA (1.9% [0.7]) or controls (1.9% [0.67]; p < 0.001 for both), but not between the last two groups (p = 0.6). Among 13 nonanemic stray cats with significant HB formation, the median (IQR) COHb concentration was 6.1% (1.2). The area under the ROC curve for COHb as a predictor of HA among all anemic cats was 0.996 (95% CI, 0.985–1), with an optimal cut-off point of 2.95% yielding a sensitivity/specificity of 95% (95% CI, 76%–99%) and 100% (95% CI, 88%–100%), respectively. Survival and COHb concentrations were not associated in either group.

Conclusions and Clinical Importance

COHb proved a useful ancillary test in cats with suspected HA. Nevertheless, endogenous COHb production occurs with the absorption of large hematomas, not studied herein, or during hemolysis irrespective of anemia. These caveats must be considered when applying the present findings to the clinical and research setting.

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引用次数: 0

Risk Factors and Long-Term Outcomes in Horses After the 2021 Outbreak of Equine Herpesvirus 1 Myeloencephalopathy, Valencia, Spain

IF 2.1 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine

Pub Date : 2025-03-07 DOI: 10.1111/jvim.70040

María de la Cuesta-Torrado, Ana Velloso Alvarez, Isabel Santiago-Llorente, Lara Armengou, Federico Nieto, José Ríos, Fátima Cruz-López, Eduard Jose-Cunilleras

Background

Equine herpesvirus myeloencephalopathy (EHM) is a persistent threat to horses, with unclear risk factors and disease severity.

Objectives

To evaluate risk factors, effective reproduction rate (Rt), and long-term athletic outcomes of an EHM outbreak.

Methods

Retrospective study of the 2021 EHM outbreak in Valencia, Spain, examining associations between risk factors (sex, age, breed, country of origin, and vaccination status) and case fatality rate, EHM development, and odds of returning to competition using odds ratios [95% CI] and Rt via the Robert Kochs Institute method.

Results

Among 191 horses, 38 (20%) were clinically normal, 13 (7%) were subclinical, and 140 (73%) presented clinical signs (89 EHM, 64%). One hundred sixty horses were isolated at the show, while 47 were treated in hospitals. The mean age was 9.8 ± 3.0 years; 85 (45%) were mares, 79 (41%) geldings, and 27 (14%) stallions. The EHM case fatality rate was 11/89 (12%). Vaccination was associated with EHM development (4.54[2.23–9.27]; OR[95% CI]; p < 0.001) and case fatality rate (3.9[1.1–14.4]; OR[95% CI]; p < 0.043). EHV-1-infected horses without EHM were more likely to return to competition (54/61; 89%) than those recovering from EHM (65/89; 73%; p = 0.024). It was initially 4.2 and decreased to < 1 within 2 weeks of the outbreak.

Conclusions

During the 2021 EHV-1 outbreak in Valencia, vaccination status appears to be associated with EHM development. Horses recovering from EHM had slightly lower chances of returning to competition than those shedding EHV-1 without EHM. The high R_t value underscores the contagious nature of EHV-1.

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引用次数: 0

Longitudinal Changes in Fecal Microbiota During Hospitalization in Horses With Different Types of Colic

IF 2.1 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine

Pub Date : 2025-03-06 DOI: 10.1111/jvim.70039

Clémence Loublier, Marcio Costa, Bernard Taminiau, Laureline Lecoq, Georges Daube, Hélène Amory, Carla Cesarini

Background

Research on fecal microbiota changes during hospitalization of horses with colic is emerging.

Objectives

Describe changes of the fecal microbiota during hospitalization of horses with colic caused by inflammatory (INFL), simple (SIMPLE), and strangulated (STR) obstructions, and investigate associations with survival.

Animals

Twenty-three horses with colic: 9 in INFL, 5 in STR, and 9 in SIMPLE groups. Seventeen horses survived, and 6 were euthanized.

Methods

Prospective observational study. Fecal samples were collected on admission (D1), on days 3 (D3) and 5 (D5). Bacterial taxonomy profiling was obtained by V1V3 16S amplicon sequencing. Data were compared using a 2-way permutational analysis of variance (PERMANOVA). Linear discriminant analysis Effect Size (LEfSE) analysis identified significant bacterial population differences, with significance set at p < 0.05 and a linear discriminant analysis (LDA) cut-off > 3.0.

Results

Alpha diversity indices remained stable during hospitalization within each colic group. However, at D5, the INFL group had significantly higher richness (p < 0.01) and diversity (Shannon, p < 0.001 and Simpson, p < 0.05) than other colic types. Beta diversity (Jaccard membership and Bray-Curtis indices) was significantly different in the INFL compared to SIMPLE and STR groups (both p < 0.001) but not between SIMPLE and STR. Beta diversity membership analysis by analysis of molecular variance (AMOVA) indicated a significant difference between survivors and non-survivors within the INFL group (p < 0.01). Increased relative abundances of Bacilliculturomica and Saccharofermentans were associated with survival.

Conclusions

Microbiota showed no significant variation over 5 days of hospitalization. Colic type influenced fecal microbiota more than hospitalization duration. Specific bacterial populations may differ between survival and non-survival groups.

{"title":"Longitudinal Changes in Fecal Microbiota During Hospitalization in Horses With Different Types of Colic","authors":"Clémence Loublier, Marcio Costa, Bernard Taminiau, Laureline Lecoq, Georges Daube, Hélène Amory, Carla Cesarini","doi":"10.1111/jvim.70039","DOIUrl":"https://doi.org/10.1111/jvim.70039","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Research on fecal microbiota changes during hospitalization of horses with colic is emerging.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Describe changes of the fecal microbiota during hospitalization of horses with colic caused by inflammatory (INFL), simple (SIMPLE), and strangulated (STR) obstructions, and investigate associations with survival.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Animals</h3>\u0000 \u0000 <p>Twenty-three horses with colic: 9 in INFL, 5 in STR, and 9 in SIMPLE groups. Seventeen horses survived, and 6 were euthanized.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Prospective observational study. Fecal samples were collected on admission (D1), on days 3 (D3) and 5 (D5). Bacterial taxonomy profiling was obtained by V1V3 16S amplicon sequencing. Data were compared using a 2-way permutational analysis of variance (PERMANOVA). Linear discriminant analysis Effect Size (LEfSE) analysis identified significant bacterial population differences, with significance set at <i>p</i> < 0.05 and a linear discriminant analysis (LDA) cut-off > 3.0.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Alpha diversity indices remained stable during hospitalization within each colic group. However, at D5, the INFL group had significantly higher richness (<i>p</i> < 0.01) and diversity (Shannon, <i>p</i> < 0.001 and Simpson, <i>p</i> < 0.05) than other colic types. Beta diversity (Jaccard membership and Bray-Curtis indices) was significantly different in the INFL compared to SIMPLE and STR groups (both <i>p</i> < 0.001) but not between SIMPLE and STR. Beta diversity membership analysis by analysis of molecular variance (AMOVA) indicated a significant difference between survivors and non-survivors within the INFL group (<i>p</i> < 0.01). Increased relative abundances of <i>Bacilliculturomica</i> and <i>Saccharofermentans</i> were associated with survival.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Microbiota showed no significant variation over 5 days of hospitalization. Colic type influenced fecal microbiota more than hospitalization duration. Specific bacterial populations may differ between survival and non-survival groups.</p>\u0000 </section>\u0000 </div>","PeriodicalId":49958,"journal":{"name":"Journal of Veterinary Internal Medicine","volume":"39 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jvim.70039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of an Automated Fluorescence Enzyme Immunoassay for Quantification of Equine Insulin and Comparison to Five Other Immunoassays

IF 2.1 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine

Pub Date : 2025-03-06 DOI: 10.1111/jvim.70038

Rose D. Nolen-Walston, Jeaneen C. Kulp, Darko Stefanovski, Andrew W. van Eps

Background

Hyperinsulinemia is an important and treatable risk factor for laminitis in horses.

Objectives

Evaluate the Tosoh AIA-360 automated fluorescence enzyme immunoassay for the measurement of serum insulin concentrations in horses, and compare it to five other immunoassays for insulin quantification.

Animals

One hundred serum samples from 83 horses were submitted for insulin measurement.

Methods

The Tosoh AIA-360 was assessed against a reference assay (radioactive immunoassay; RIA). Using the same samples, TOS-FEIA, ELISA, and three chemiluminescent immunoassays (CLIA) were assessed for correlation and agreement with RIA.

Results

The TOS-FEIA showed excellent correlation with RIA (r² = 0.94, p < 0.0001) and good agreement, with a Bland–Altman constant bias (limits of agreement) of −23.8 μIU/mL (−74.6 to 27.0) and Passing–Bablok fit of y = −8.9 + 0.78x. Mean coefficients of variation were 1.8% for intra-assay and 5.7% for inter-assay precision, with mean recovery upon dilution of 104.2%. The assay comparison yielded good or excellent agreement (constant bias, limits of agreement) with RIA in the < 100 μIU/mL cohort for the ELISA (−7.0, −21.4 to 7.4) and the Cobas e CLIA (−31.4, −60.9 to −1.6). Spuriously high results (2 to > 10-fold of RIA result) were obtained in approximately 10% of results from both Immulite 2000 and 2000XPi CLIA analyzers, rendering the agreement poor.

Conclusions and Clinical Importance

The TOS-FEIA had acceptable accuracy and precision for clinical use, including at concentrations of insulin < 100 μIU/mL. The ELISA and one CLIA (Cobas e) showed acceptable accuracy, but the Cobas e demonstrated marked bias compared with RIA. Both Immulite CLIA assays exhibited unacceptable accuracy.

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引用次数: 0

Renin-Angiotensin-Aldosterone System Profiling in Horses Before and After Exercise

IF 2.1 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine

Pub Date : 2025-03-06 DOI: 10.1111/jvim.70036

Todd Holbrook, Jorge Hernandez, Taralyn McCarrel, Guy Lester, Margaret Sleeper, Oliver Domenig, Darcy Adin

Background

The impact of exercise on the classical and alternative renin-angiotensin-aldosterone system (RAAS) pathways has not been studied in horses.

Hypothesis

We hypothesized that exercise would activate both RAAS pathways and that endurance exercise would cause more activation of the classical pathway compared to short-duration, high-intensity exercise in horses.

Animals

Twenty-five horses (21 client-owned and 4 research) were included in 4 exercise groups (10 Arabians, 50-mile ride [A-E]; 4 thoroughbreds, 1-mile treadmill exercise [TB-TM]; 5 thoroughbreds, 1–1/16th-mile race [TB-R]; and 6 quarter horses 330-500-yard race [QH-R]).

Methods

Blood was collected before and after exercise. Equilibrium analysis was performed to measure serum RAAS metabolites and enzyme activities. The components of the RAAS pathways were compared before and after exercise. Post/pre-exercise ratios for each variable were compared among exercise groups. Data were reported as median (first, third quartiles; pre vs. post) and p < 0.05 was considered significant.

Results

Exercise increased classical RAAS metabolites (pmol/L; angiotensin I, 2.5 [2.5, 2.5] vs. 8.2 [2.5, 19.0]; angiotensin II, 10.2 [6.0, 21.9] vs. 53.0 [37.4, 95.8]; aldosterone, 83.8 [53.4, 149.5] vs. 170.6 [112.2, 251.7]); alternative RAAS metabolites (pmol/L; angiotensin 1–7, 1.5 [1.5, 1.5] vs. 5.1 [1.5, 12.5]; angiotensin 1–5, 2.5 [2.5, 7.3] vs. 14.9 [9.0, 25.4]) and angiotensin-converting enzyme-2 activity (ng/mL; 16.6 [13.9, 20.4] vs. 25.2 [20.2, 33.0]; p < 0.001) for all horses. Angiotensin 1–7 ratios were higher for TB-R compared with TB-TM and A-E (p < 0.001).

Conclusions and Clinical Importance

Both classical and alternative RAAS pathways increase after exercise in horses.

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引用次数: 0

The Acute Patient Physiologic and Laboratory Evaluation Score and Other Prognostic Factors in Dogs With Diabetic Ketoacidosis

IF 2.1 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine

Pub Date : 2025-03-06 DOI: 10.1111/jvim.70044

Sarah M. Edwards, Rebecka S. Hess

Background

Acute patient physiologic and laboratory evaluation (APPLE) scores have not been reported in dogs with diabetic ketoacidosis (DKA).

Hypothesis

The APPLE scores will be higher in non-survivors compared with survivors, and higher scores will predict increased mortality in dogs with DKA.

Animals

Eighty-five dogs with DKA; 58 survivors (68%) and 27 non-survivors (32%).

Methods

Retrospective study. The APPLE scores were entered into a multivariate logistic regression model for mortality prediction. Variables related to DKA diagnosis also were examined as mortality predictors. If variables predicted mortality, an empirical optimal cut point, corresponding area under the receiver operating curve (AUC), and sensitivity and specificity for predicting mortality were calculated.

Results

Mean 10-variable APPLE_full and median 5-variable APPLE_fast scores were higher in non-survivors (32 ± 10 and 11; range, 3–29, respectively) compared with survivors (25 ± 8; p < 0.001 and 7; range, 0–24; p = 0.02, respectively). The APPLE_full score predicted mortality (p = 0.03). The AUC for the APPLE_full as a predictor of mortality was 0.67 and at the empirical optimal cutpoint of 23.5 the sensitivity and specificity of the APPLE_full score for mortality prediction were 85% and 48%, respectively. Beta-hydroxybutyrate concentration (BOHB) also predicted mortality (p = 0.02). The AUC for BOHB as a mortality predictor was 0.75 and at the empirical optimal cutpoint of 4.75 the sensitivity and specificity of BOHB for mortality prediction were 58% and 92%, respectively.

Conclusions and Clinical Importance

The APPLE_full score and BOHB predict mortality in dogs with DKA and can be used to stratify DKA dogs into appropriate survival groups.

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引用次数: 0

Prognostic Roles of Trace Element and Cobalamin Concentrations in Dogs With Parvoviral Enteritis

IF 2.1 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine

Pub Date : 2025-03-06 DOI: 10.1111/jvim.70046

Kerim Emre Yanar, Selin Sinem Sümbül Laçin, Mustafa Sinan Aktaş, Mehmet Özkan Timurkan, Hakan Aydın

<div> <section> <h3> Background</h3> <p>The trace elements copper (Cu), zinc (Zn), and selenium (Se) have been the focus of research into their potential roles in the prognosis of gastrointestinal disorders in humans.</p> </section> <section> <h3> Objective</h3> <p>Evaluation of the predictive potential serum concentrations of Cu, Zn, Cu/Zn, Se, and cobalamin as possible prognostic indicators in dogs with parvoviral enteritis (CPV).</p> </section> <section> <h3> Animals</h3> <p>Client-owned dogs diagnosed with CPV (<i>n</i> = 20) and healthy controls (<i>n</i> = 10).</p> </section> <section> <h3> Methods</h3> <p>A case-controlled study. Serum concentrations of Cu and Zn were measured using a spectrophotometric method; serum Se levels were determined by mass spectrophotometry; and serum cobalamin concentrations were assessed using a chemiluminescent immunoassay method. The Mann–Whitney <i>U</i> test was employed to compare subgroup medians.</p> </section> <section> <h3> Results</h3> <p>Upon admission, surviving dogs with CPV (<i>n</i> = 10) exhibited higher serum Cu concentrations (median = 154.24; range = 60.15–188.46 μg/dL) and Cu/Zn ratios (median = 1.52; range = 0.67–2.45), alongside lower serum Zn concentrations (median = 88.05; range = 51.3–129.2 μg/dL) and cobalamin levels (median = 252.5; range = 111–396 pg/mL), compared to the control group (Cu, median = 72.12; range = 47.04–90.26 μg/dL), Zn (median = 184.2; range = 73.0–262.7 μg/dL), Cu/Zn (median = 0.37; range = 0.26–0.73), cobalamin (median = 638.5; range = 306.0–1016 pg/mL). Additionally, non-surviving dogs (<i>n</i> = 10) exhibited markedly higher serum Cu concentrations (median = 193.5; range = 125.0–229.0 μg/dL) and Cu/Zn ratios (median = 5.45; range = 1.95–9.23), and significantly lower serum Zn (median = 37.75; range = 24.8–71.6 μg/dL), Se (median = 52.45; range = 21.27–91 μg/L), and cobalamin levels (median = 52.2; range = 20.0–147.0 pg/mL) compared to both survivors and controls.</p> </section> <section> <h3> Conclusions and Clinical Importance</h3> <p>Statistical variations in serum concentrations of Cu, Zn, and cobalamin, alongside Cu/Zn ratios, were observed among survivors, non-survivors, and controls (control-survivor and survivor-non-survivor: <i>p</i> < 0.05 and control-non-survivor: <i>p</i> < 0.01), which might suggest their potential prognostic value in CPV.</p>

{"title":"Prognostic Roles of Trace Element and Cobalamin Concentrations in Dogs With Parvoviral Enteritis","authors":"Kerim Emre Yanar, Selin Sinem Sümbül Laçin, Mustafa Sinan Aktaş, Mehmet Özkan Timurkan, Hakan Aydın","doi":"10.1111/jvim.70046","DOIUrl":"https://doi.org/10.1111/jvim.70046","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The trace elements copper (Cu), zinc (Zn), and selenium (Se) have been the focus of research into their potential roles in the prognosis of gastrointestinal disorders in humans.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Evaluation of the predictive potential serum concentrations of Cu, Zn, Cu/Zn, Se, and cobalamin as possible prognostic indicators in dogs with parvoviral enteritis (CPV).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Animals</h3>\u0000 \u0000 <p>Client-owned dogs diagnosed with CPV (<i>n</i> = 20) and healthy controls (<i>n</i> = 10).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A case-controlled study. Serum concentrations of Cu and Zn were measured using a spectrophotometric method; serum Se levels were determined by mass spectrophotometry; and serum cobalamin concentrations were assessed using a chemiluminescent immunoassay method. The Mann–Whitney <i>U</i> test was employed to compare subgroup medians.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Upon admission, surviving dogs with CPV (<i>n</i> = 10) exhibited higher serum Cu concentrations (median = 154.24; range = 60.15–188.46 μg/dL) and Cu/Zn ratios (median = 1.52; range = 0.67–2.45), alongside lower serum Zn concentrations (median = 88.05; range = 51.3–129.2 μg/dL) and cobalamin levels (median = 252.5; range = 111–396 pg/mL), compared to the control group (Cu, median = 72.12; range = 47.04–90.26 μg/dL), Zn (median = 184.2; range = 73.0–262.7 μg/dL), Cu/Zn (median = 0.37; range = 0.26–0.73), cobalamin (median = 638.5; range = 306.0–1016 pg/mL). Additionally, non-surviving dogs (<i>n</i> = 10) exhibited markedly higher serum Cu concentrations (median = 193.5; range = 125.0–229.0 μg/dL) and Cu/Zn ratios (median = 5.45; range = 1.95–9.23), and significantly lower serum Zn (median = 37.75; range = 24.8–71.6 μg/dL), Se (median = 52.45; range = 21.27–91 μg/L), and cobalamin levels (median = 52.2; range = 20.0–147.0 pg/mL) compared to both survivors and controls.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions and Clinical Importance</h3>\u0000 \u0000 <p>Statistical variations in serum concentrations of Cu, Zn, and cobalamin, alongside Cu/Zn ratios, were observed among survivors, non-survivors, and controls (control-survivor and survivor-non-survivor: <i>p</i> < 0.05 and control-non-survivor: <i>p</i> < 0.01), which might suggest their potential prognostic value in CPV.</p>\u0000 ","PeriodicalId":49958,"journal":{"name":"Journal of Veterinary Internal Medicine","volume":"39 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jvim.70046","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0