Acoustic vibration promotes in vitro expansion of human embryonic stem cells.

IF 1.5 Q4 CELL BIOLOGY American journal of stem cells Pub Date : 2024-06-15 eCollection Date: 2024-01-01 DOI:10.62347/PJFC2708
Xiangyue Hu, Haoyun Duan, Dulei Zou, Chunxiao Dong, Yani Wang, Yao Wang, Zongren Li, Zongyi Li
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Abstract

Objectives: This study aimed to investigate the effect of acoustic vibration on the pluripotency of human embryonic stem cells (hESCs) and evaluate cell proliferation and self-renewal ability post-treatment.

Methods: The human ES cell line H1 was used for the experiments. hESCs were treated with an acoustic vibration device. Their proliferative ability was subsequently detected using a colony formation assay, while the expression of pluripotency-related markers was detected via immunofluorescence staining. Finally, changes in gene expression levels were examined using quantitative polymerase chain reaction (qPCR) in the presence of appropriate primers.

Results: Compared with normal cells in the control group, the morphology of experimental cells subjected to acoustic vibration did not significantly change. Contrastingly, the colony-forming efficiency of the experimental cells significantly increased. Immunofluorescence staining results showed the cells in experimental group were positive for the pluripotency markers NANOG, octamer-binding transcription factor 4 gene (OCT4), and SRY (sex determining region Y)-box 2 (SOX2). In addition, the expression levels of pluripotency genes NANOG, OCT4, SOX2, and Yes-associated protein (YAP)-related genes were up-regulated following acoustic vibration.

Conclusions: Our results revealed that acoustic vibration enhanced the proliferative ability of hESCs and increased the expression levels of NANOG, OCT4, SOX2, and YAP-related genes, indicating that acoustic vibration can optimize the self-renewal ability of hESCs and that the YAP signaling pathway may play a critical role in the functional process of acoustic vibration.

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声学振动促进人类胚胎干细胞的体外扩增。
研究目的本研究旨在探讨声学振动对人类胚胎干细胞(hESCs)多能性的影响,并评估处理后细胞的增殖和自我更新能力:实验使用人类 ES 细胞株 H1。方法:实验采用人 ES 细胞系 H1,用声波振动装置处理 hESC,然后用集落形成试验检测其增殖能力,并通过免疫荧光染色检测多能性相关标记物的表达。最后,在适当引物的作用下,使用定量聚合酶链反应(qPCR)检测基因表达水平的变化:结果:与对照组的正常细胞相比,声学振动实验组细胞的形态没有明显变化。相反,实验组细胞的集落形成效率明显提高。免疫荧光染色结果显示,实验组细胞的多能性标志物NANOG、八聚体结合转录因子4基因(OCT4)和SRY(性别决定区Y)-box 2(SOX2)呈阳性。此外,多能性基因NANOG、OCT4、SOX2和Yes相关蛋白(YAP)相关基因的表达水平在声学振动后上调:我们的研究结果表明,声学振动增强了 hESCs 的增殖能力,提高了 NANOG、OCT4、SOX2 和 YAP 相关基因的表达水平,表明声学振动可以优化 hESCs 的自我更新能力,YAP 信号通路可能在声学振动的功能过程中发挥了关键作用。
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Cellular therapies for idiopathic pulmonary fibrosis: current progress and future prospects. Complete lasting reversal of polycystic ovary syndrome from intravenous umbilical cord derived mesenchymal stem cell infusion. Examining the level of inflammatory cytokines TNF-α and IL-8 produced by osteoblasts differentiated from dental pulp stem cells. Exploring the application of stem cell technology in treating sensorineural hearing loss. Acoustic vibration promotes in vitro expansion of human embryonic stem cells.
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