Roughness affects the response of human fibroblasts and macrophages to sandblasted abutments.

IF 2.9 4区 医学 Q3 ENGINEERING, BIOMEDICAL BioMedical Engineering OnLine Pub Date : 2024-07-17 DOI:10.1186/s12938-024-01264-6
Francisco Romero-Gavilán, Carlos Arias-Mainer, Andreia Cerqueira, David Peñarrocha-Oltra, Juan Carlos Bernabeu-Mira, Iñaki García-Arnáez, Félix Elortza, María Muriach, Mariló Gurruchaga, Isabel Goñi, Julio Suay
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Abstract

Background: A strong seal of soft-tissue around dental implants is essential to block pathogens from entering the peri-implant interface and prevent infections. Therefore, the integration of soft-tissue poses a challenge in implant-prosthetic procedures, prompting a focus on the interface between peri-implant soft-tissues and the transmucosal component. The aim of this study was to analyse the effects of sandblasted roughness levels on in vitro soft-tissue healing around dental implant abutments. In parallel, proteomic techniques were applied to study the interaction of these surfaces with human serum proteins to evaluate their potential to promote soft-tissue regeneration.

Results: Grade-5 machined titanium discs (MC) underwent sandblasting with alumina particles of two sizes (4 and 8 μm), resulting in two different surface types: MC04 and MC08. Surface morphology and roughness were characterised employing scanning electron microscopy and optical profilometry. Cell adhesion and collagen synthesis, as well as immune responses, were assessed using human gingival fibroblasts (hGF) and macrophages (THP-1), respectively. The profiles of protein adsorption to the surfaces were characterised using proteomics; samples were incubated with human serum, and the adsorbed proteins analysed employing nLC-MS/MS. hGFs exposed to MC04 showed decreased cell area compared to MC, while no differences were found for MC08. hGF collagen synthesis increased after 7 days for MC08. THP-1 macrophages cultured on MC04 and MC08 showed a reduced TNF-α and increased IL-4 secretion. Thus, the sandblasted topography led a reduction in the immune/inflammatory response. One hundred seventy-six distinct proteins adsorbed on the surfaces were identified. Differentially adsorbed proteins were associated with immune response, blood coagulation, angiogenesis, fibrinolysis and tissue regeneration.

Conclusions: Increased roughness through MC08 treatment resulted in increased collagen synthesis in hGF and resulted in a reduction in the surface immune response in human macrophages. These results correlate with the changes in protein adsorption on the surfaces observed through proteomics.

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粗糙度会影响人类成纤维细胞和巨噬细胞对喷砂基台的反应。
背景:牙科种植体周围软组织的牢固密封对于阻止病原体进入种植体周围界面和预防感染至关重要。因此,在种植修复过程中,软组织的整合是一项挑战,促使人们关注种植体周围软组织与经粘膜组件之间的界面。本研究旨在分析喷砂粗糙度对种植体基台周围软组织体外愈合的影响。同时,应用蛋白质组学技术研究这些表面与人血清蛋白的相互作用,以评估其促进软组织再生的潜力:结果:5 级机加工钛盘(MC)经过两种尺寸(4 微米和 8 微米)的氧化铝颗粒喷砂处理,形成两种不同的表面类型:MC04 和 MC08。采用扫描电子显微镜和光学轮廓仪对表面形态和粗糙度进行了表征。分别使用人牙龈成纤维细胞(hGF)和巨噬细胞(THP-1)对细胞粘附和胶原合成以及免疫反应进行了评估。使用蛋白质组学分析了表面吸附蛋白质的特征;样品与人血清一起培养,并使用 nLC-MS/MS 分析吸附的蛋白质。与 MC04 相比,接触 MC04 的 hGF 细胞面积减少,而接触 MC08 的 hGF 细胞面积则没有变化。在 MC04 和 MC08 上培养的 THP-1 巨噬细胞显示 TNF-α 分泌减少,IL-4 分泌增加。由此可见,喷砂地形减少了免疫/炎症反应。研究人员对表面吸附的 176 种不同蛋白质进行了鉴定。不同的吸附蛋白与免疫反应、血液凝固、血管生成、纤维蛋白溶解和组织再生有关:结论:通过 MC08 处理增加粗糙度可增加 hGF 中胶原蛋白的合成,并降低人体巨噬细胞的表面免疫反应。这些结果与蛋白质组学观察到的表面蛋白质吸附变化相关。
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来源期刊
BioMedical Engineering OnLine
BioMedical Engineering OnLine 工程技术-工程:生物医学
CiteScore
6.70
自引率
2.60%
发文量
79
审稿时长
1 months
期刊介绍: BioMedical Engineering OnLine is an open access, peer-reviewed journal that is dedicated to publishing research in all areas of biomedical engineering. BioMedical Engineering OnLine is aimed at readers and authors throughout the world, with an interest in using tools of the physical and data sciences and techniques in engineering to understand and solve problems in the biological and medical sciences. Topical areas include, but are not limited to: Bioinformatics- Bioinstrumentation- Biomechanics- Biomedical Devices & Instrumentation- Biomedical Signal Processing- Healthcare Information Systems- Human Dynamics- Neural Engineering- Rehabilitation Engineering- Biomaterials- Biomedical Imaging & Image Processing- BioMEMS and On-Chip Devices- Bio-Micro/Nano Technologies- Biomolecular Engineering- Biosensors- Cardiovascular Systems Engineering- Cellular Engineering- Clinical Engineering- Computational Biology- Drug Delivery Technologies- Modeling Methodologies- Nanomaterials and Nanotechnology in Biomedicine- Respiratory Systems Engineering- Robotics in Medicine- Systems and Synthetic Biology- Systems Biology- Telemedicine/Smartphone Applications in Medicine- Therapeutic Systems, Devices and Technologies- Tissue Engineering
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