Dipankar Hazarika, Durlabh Prasad Borah, J. S. Boruah, Yasmin Begum, Anisha Sultana, S. Tamuly, Kamal Behari Devchoudhuri, Probodh Borah, Dhruba Jyoti Kalita
{"title":"Toxicological Analysis of Nanoparticles and Microparticles Used as Oral Vaccine Delivery Systems using Vero Cell","authors":"Dipankar Hazarika, Durlabh Prasad Borah, J. S. Boruah, Yasmin Begum, Anisha Sultana, S. Tamuly, Kamal Behari Devchoudhuri, Probodh Borah, Dhruba Jyoti Kalita","doi":"10.18805/ijar.b-5246","DOIUrl":null,"url":null,"abstract":"Background: The present study was carried out to evaluate in-vitro toxicity associated with chitosan nanoparticles, Gantrez® nanoparticles and poly-lactide co-glycolide (PLG) microparticle in Vero cell line. The cytotoxicity of all three micro/nano-particles was assessed using different concentration. Methods: For each concentration of these delivery systems, the confluent monolayer of Vero cells was treated for a period of 48 hours and studied for morphological alteration and cell survivability after the treatment. Results: It was observed that the different concentrations of chitosan nanoparticles and Gantrez® nanoparticles did not have significant effect on the cell viability as evident from the non-significant difference between the OD540 of formazan product formed from MTT in treated and untreated cells. The concentration of chitosan nanoparticles and Gantrez® nanoparticles up to 1000 µg/ml did not have any influence in cellular metabolic activities and viability. However, a slight reduction (statistically insignificant) in the cellular viability and metabolic activities were observed when PLG microparticles were used at 1000 µg/ml.","PeriodicalId":507727,"journal":{"name":"Indian Journal of Animal Research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Animal Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18805/ijar.b-5246","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Background: The present study was carried out to evaluate in-vitro toxicity associated with chitosan nanoparticles, Gantrez® nanoparticles and poly-lactide co-glycolide (PLG) microparticle in Vero cell line. The cytotoxicity of all three micro/nano-particles was assessed using different concentration. Methods: For each concentration of these delivery systems, the confluent monolayer of Vero cells was treated for a period of 48 hours and studied for morphological alteration and cell survivability after the treatment. Results: It was observed that the different concentrations of chitosan nanoparticles and Gantrez® nanoparticles did not have significant effect on the cell viability as evident from the non-significant difference between the OD540 of formazan product formed from MTT in treated and untreated cells. The concentration of chitosan nanoparticles and Gantrez® nanoparticles up to 1000 µg/ml did not have any influence in cellular metabolic activities and viability. However, a slight reduction (statistically insignificant) in the cellular viability and metabolic activities were observed when PLG microparticles were used at 1000 µg/ml.