In vitro Study on the Immunomodulatory and Anti-inflammatory Effect of Soregen® Technology Water in Broiler and Layer Hens Peripheral Blood Mononuclear Cells

Abstract

Background: Peripheral blood mononuclear cells (PBMCs) represent an attractive tissue source in pharmacogenomics and molecular and immunologic studies. In order to investigate the changes in biological effects, water samples were exposed to a specific quantum entanglement signal from the SoRegen chip for 48 hours. This study aimed to evaluate the immunomodulatory and anti-inflammatory effects of SoRegen Technology in broiler and layer hens PBMCs. Methods: Six-week-old Arbor Acress broiler chickens and 66-week-old layer hens Lohmann were used for the establishment of an in vitro cell culture model with chicken PBMCs. Result: The result indicates that post-treated water media enhanced the proliferative properties of broiler and layer hens PBMCs. The mRNA and protein expression of immune-modulating and pro-inflammatory cytokines: IL-2, IL-6, iNOS, IFNγ, TNFα and NFκB exhibited a marked increase following stimulation of the cells by LPS and Con A when compared with the non-stimulated cells. However, stimulated cells grown in structured water media showed a strikingly decreased expression of pro-inflammatory cytokines, except for the upregulation of IFNg expression in broiler PBMCs but not in layer hens PBMCs. It can be concluded that SoRegen® Technology water had anti-inflammatory activities with potential clinical immunomodulatory effects in younger chicks.