A. Shitikova, M. Atroshchenko, V. Zvyagina, E. Belskikh, A.I. Romanova
{"title":"Importance of L-carnitine as Biochemical Marker for Semen Quality Preservation after Cryopreservation in Stallions","authors":"A. Shitikova, M. Atroshchenko, V. Zvyagina, E. Belskikh, A.I. Romanova","doi":"10.18805/ijar.bf-1792","DOIUrl":null,"url":null,"abstract":"Background: The aim of this work is to study the significance of carnitine fractions and NO metabolites for the assessment of spermatozoa resistance to cryopreservation, for which purpose these parameters were investigated in seminal plasma of animals divided according to the indices of spermatozoa of stallions subjected to cryopreservation. Methods: There were 18 breeding stallions with an average age of 11.28±5.46 years, all 18 were of Arabian breed. Using cluster analysis of indicators of semen quality, animals were identified into two groups: with high and low sperm resistance to cryopreservation-thawing. We assessed standard quality parameters in fresh and cryopreserved sperm, as well as carnitine and its fractions and NO metabolites in stallions’ seminal plasma, steroid hormones level and carnitine fractions in blood serum. Data were analyzed using JASP. Differences at p less than 0.05 were considered statistically significant. Result: Seminal plasma obtained from animals from the cluster with low motility after cryopreservation-thawing was characterized by a lower concentration of nitric oxide metabolites in 1,79 time (p=0.0415) and free carnitine in 1,64 time (p=0.0349). Positive moderate strength correlation were established between the level of seminal plasma free carnitine and the percentage of live spermatozoa after sperm cryopreservation-thawing.\n","PeriodicalId":507727,"journal":{"name":"Indian Journal of Animal Research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Animal Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18805/ijar.bf-1792","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Background: The aim of this work is to study the significance of carnitine fractions and NO metabolites for the assessment of spermatozoa resistance to cryopreservation, for which purpose these parameters were investigated in seminal plasma of animals divided according to the indices of spermatozoa of stallions subjected to cryopreservation. Methods: There were 18 breeding stallions with an average age of 11.28±5.46 years, all 18 were of Arabian breed. Using cluster analysis of indicators of semen quality, animals were identified into two groups: with high and low sperm resistance to cryopreservation-thawing. We assessed standard quality parameters in fresh and cryopreserved sperm, as well as carnitine and its fractions and NO metabolites in stallions’ seminal plasma, steroid hormones level and carnitine fractions in blood serum. Data were analyzed using JASP. Differences at p less than 0.05 were considered statistically significant. Result: Seminal plasma obtained from animals from the cluster with low motility after cryopreservation-thawing was characterized by a lower concentration of nitric oxide metabolites in 1,79 time (p=0.0415) and free carnitine in 1,64 time (p=0.0349). Positive moderate strength correlation were established between the level of seminal plasma free carnitine and the percentage of live spermatozoa after sperm cryopreservation-thawing.
背景:这项工作的目的是研究肉碱组分和 NO 代谢物对评估精子抗冷冻能力的意义,为此研究了根据接受冷冻保存的种公马精子指数划分的动物精浆中的这些参数。研究方法18匹种公马的平均年龄为(11.28±5.46)岁,均为阿拉伯种。通过对精液质量指标进行聚类分析,将动物分为两组:精子对冷冻解冻的耐受性高和耐受性低。我们评估了新鲜精子和冷冻保存精子的标准质量参数,以及种公马精浆中的肉碱及其组分和氮氧化物代谢物、血清中的类固醇激素水平和肉碱组分。数据使用 JASP 进行分析。P小于0.05的差异被认为具有统计学意义。结果从低温解冻后运动能力低的组群中获得的精浆的特点是,一氧化氮代谢物的浓度在1.79倍时较低(p=0.0415),游离肉碱的浓度在1.64倍时较低(p=0.0349)。精子冷冻-解冻后,精浆游离肉碱水平与活精子百分比之间呈中度正相关。