Genome-wide identification of R2R3-MYB family in Eriobotrya japonica and functional analysis of EjMYB5 involved in proanthocyanidin biosynthesis

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Plant Science Pub Date : 2024-07-18 DOI:10.1016/j.plantsci.2024.112198

Yin Zhang , Luyan Hu , Shuming Wang , Xiuhong Gou , Qigao Guo , Guolu Liang

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Abstract

Loquat (Eriobotrya japonica Lindl.) is a popular fruit and medicinal plant. Proanthocyanidins (PAs), as one of the main types of flavonoids, are the key components of loquat fruit quality and medicinal properties. However, the identification of transcription factors (TFs) involved in PA accumulation in loquat remains limited. R2R3-MYB TFs play key regulatory role in PA accumulation in plants. In this study, 190 R2R3-MYB TFs were identified in loquat genome. Combined with transcriptome data, R2R3-MYB TF EjMYB5 involved in PA accumulation in loquat was isolated. EjMYB5 was transcriptional activator localized to nucleus. Expression of EjMYB5 was closely related to PA accumulation in loquat fruits. Heterogenous overexpression of EjMYB5 in tomato (Solanum lycopersicum) inhibited anthocyanin accumulation and promoted PA accumulation. Additionally, transient overexpression of EjMYB5 in tobacco (Nicotiana benthamiana) leaves promoted PA accumulation by upregulating flavonoid biosynthesis genes (NtDFR, NtANS, and NtLAR). Transcriptome analysis of EjMYB5-overexpressing tomato fruits suggested that EjMYB5 was involved in several biological pathways, including lipid metabolism, MAPK signaling, phenylpropanoid biosynthesis, and flavonoid biosynthesis. Collectively, our findings provided basic data for further analysis the function of R2R3-MYB TFs in loquat, and revealed that EjMYB5 functioned as PA accumulation in loquat.

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粳稻 R2R3-MYB 家族的全基因组鉴定及参与原花青素生物合成的 EjMYB5 的功能分析。

枇杷（Eriobotrya japonica Lindl.）是一种广受欢迎的水果和药用植物。原花青素（PA）是黄酮类化合物的主要类型之一，是枇杷果实品质和药用价值的关键成分。然而，参与枇杷PA积累的转录因子（TFs）的鉴定仍然有限。R2R3-MYB TFs 在植物 PA 积累过程中起着关键的调控作用。本研究在枇杷基因组中鉴定了 190 个 R2R3-MYB TFs。结合转录组数据，分离出参与枇杷PA积累的R2R3-MYB TF EjMYB5。EjMYB5 是定位于细胞核的转录激活因子。EjMYB5 的表达与枇杷果实中 PA 的积累密切相关。EjMYB5 在番茄（Solanum lycopersicum）中的异源过表达抑制了花青素的积累，促进了 PA 的积累。此外，在烟草（Nicotiana benthamiana）叶片中瞬时过表达 EjMYB5 可通过上调类黄酮生物合成基因（NtDFR、NtANS 和 NtLAR）促进 PA 的积累。对表达 EjMYB5 的番茄果实的转录组分析表明，EjMYB5 参与了多个生物学通路，包括脂质代谢、MAPK 信号转导、苯丙类生物合成和黄酮类生物合成。总之，我们的研究结果为进一步分析枇杷中R2R3-MYB TFs的功能提供了基础数据，并揭示了EjMYB5在枇杷中的PA积累功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.