Biomaterial Fg/P(LLA-CL) regulates macrophage polarization and recruitment of mesenchymal stem cells after endometrial injury

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL Journal of Materials Science: Materials in Medicine Pub Date : 2024-07-29 DOI:10.1007/s10856-024-06807-w

Sirui Song, Anfeng Wang, Siyu Wu, Huaifang Li, Hongbing He

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Abstract

The process of endometrial repair after injury involves the synergistic action of various cells including immune cells and stem cells. In this study, after combing Fibrinogen(Fg) with poly(L-lacticacid)-co-poly(ε-caprolactone)(P(LLA-CL)) by electrospinning, we placed Fg/P(LLA-CL) into the uterine cavity of endometrium-injured rats, and bioinformatic analysis revealed that Fg/P(LLA-CL) may affect inflammatory response and stem cell biological behavior. Therefore, we verified that Fg/P(LLA-CL) could inhibit the lipopolysaccharide (LPS)-stimulated macrophages from switching to the pro-inflammatory M1 phenotype in vitro. Moreover, in the rat model of endometrial injury, Fg/P(LLA-CL) effectively promoted the polarization of macrophages towards the anti-inflammatory M2 phenotype and enhanced the presence of mesenchymal stem cells at the injury site. Overall, Fg/P(LLA-CL) exhibits significant influence on macrophage polarization and stem cell behavior in endometrial injury, justifying further exploration for potential therapeutic applications in endometrial and other tissue injuries.

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生物材料Fg/P(LLA-CL)可调节巨噬细胞极化和子宫内膜损伤后间充质干细胞的招募。

子宫内膜损伤后的修复过程涉及免疫细胞和干细胞等多种细胞的协同作用。本研究将纤溶酶原（Fg）与聚（L-乳酸）-共聚（ε-己内酯）（P(LLA-CL)）通过电纺结合后，将Fg/P(LLA-CL)置入子宫内膜损伤大鼠的宫腔，生物信息学分析表明Fg/P(LLA-CL)可能影响炎症反应和干细胞生物学行为。因此，我们在体外验证了Fg/P(LLA-CL)能抑制脂多糖（LPS）刺激的巨噬细胞向促炎的M1表型转换。此外，在大鼠子宫内膜损伤模型中，Fg/P(LLA-CL)能有效促进巨噬细胞向抗炎M2表型极化，并增强损伤部位间充质干细胞的存在。总之，Fg/P(LLA-CL)对子宫内膜损伤中巨噬细胞的极化和干细胞行为有显著影响，值得进一步探索其在子宫内膜和其他组织损伤中的潜在治疗应用。

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来源期刊

Journal of Materials Science: Materials in Medicine 工程技术-材料科学：生物材料

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

3.5 months

期刊介绍： The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.