Germination and Culturability after UV Irradiation of Metarhizium anisopliae Native from Soils of Tropical Cattle Farms

Microbiology Research Pub Date : 2024-07-25 DOI:10.3390/microbiolres15030089

M. Alonso-Díaz, María de Lourdes Lozano-Velázquez, I. García-Galicia, A. Fernández-Salas

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Abstract

The use of entomopathogenic fungi (EF) is a promising alternative for the control of Rhipicephalus microplus, an important tick affecting cattle globally. This study aimed to evaluate the effect of ultraviolet irradiation (UV) exposure on the percentage of conidia germination and number of colony-forming units of eight strains of Metarhizium anisopliae (MaV55, MaV35, MaV31, MaV25, MaV13, Ma08, MaV05, and MaV02). The UV (UV-A and UV-A+B) irradiation was carried out with an ultraviolet radiation emission lamp. The conidia of each strain were exposed to the UV irradiation treatments for 3 h. MaV25, MaV08, MaV05, MaV13, and MaV31 showed higher tolerance to UV-A radiation exposure, as assessed by conidia germination. UV-A+B radiation decreased the germination percentage of all the M. anisopliae strains. The eight evaluated strains showed good tolerance to UV-A radiation, as assessed by the development of colony-forming units (CFU). UV-A+B radiation did not significantly affect (p > 0.05) the count of the CFU of six of the M. anisopliae strains evaluated (MaV35, MaV13, MaV08, MaV05, MaV31, and MaV02). The novel findings of the UV-tolerant M. anisopliae strains may potentially improve the effectiveness of EF under environmental conditions. Integral research under real tropical conditions is advised to evaluate the effectiveness of the EF strains.

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热带养牛场土壤中土生土长的 Metarhizium anisopliae 经紫外线照射后的发芽率和可培养性

使用昆虫病原真菌（EF）是一种很有前景的替代方法，可用于控制全球重要的牛蜱--Rhipicephalus microplus。本研究旨在评估紫外线照射（UV）对八株拟黑僵菌（MaV55、MaV35、MaV31、MaV25、MaV13、Ma08、MaV05 和 MaV02）的分生孢子发芽率和菌落形成单位数的影响。紫外线（UV-A 和 UV-A+B）照射是用紫外线辐射发射灯进行的。根据分生孢子萌发情况的评估，MaV25、MaV08、MaV05、MaV13 和 MaV31 对 UV-A 辐射照射表现出较高的耐受性。UV-A+B 辐射降低了所有 M. anisopliae 菌株的发芽率。根据菌落形成单位（CFU）的发育情况，8 个受评估菌株对 UV-A 辐射表现出良好的耐受性。UV-A+B 辐射对所评估的六种 M. anisopliae 菌株（MaV35、MaV13、MaV08、MaV05、MaV31 和 MaV02）的菌落形成单位计数没有明显影响（p > 0.05）。耐紫外线 M. anisopliae 菌株的新发现可能会提高环境条件下 EF 的有效性。建议在实际热带条件下进行综合研究，以评估 EF 菌株的有效性。

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Microbiology Research

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