{"title":"Development of a Validated RP-HPLC/UV Method for the Quantitative Determination of Tyrosine Kinase RET Inhibitor: Selpercatinib in Capsule Formulation","authors":"Paka Ramya, Medidi Srinivas, Bula Udaya Kumari","doi":"10.22270/jddt.v14i7.6702","DOIUrl":null,"url":null,"abstract":"A validated RP-HPLC/UV method was developed to estimate the selpercatinib in capsules. The wavelength chosen for detection was at 248 nm. The RP-HPLC separation was carried out using a Hypersil ODS C18 column (250×4.6 mm, 5 μm) with a mobile phase consisting of 0.2% TFA (pH 6.5) and acetonitrile in a ratio of 70:30 v/v. The flow rate was set at 1 ml/min. The retention time for selpercatinib was determined to be 3.012 min. Linearity was detected within the concentration range of 2.5-15 μg/mL for selpercatinib. The approach has been confirmed to be linear, accurate, precise, robust, and has established limits of detection and quantitation. The established procedure was uncomplicated, cost-effective, and suitable for the routine analysis of selpercatinib in capsule dosage form. \nKeywords: Selpercatinib, RP-HPLC and Validation.","PeriodicalId":15622,"journal":{"name":"Journal of Drug Delivery and Therapeutics","volume":" 25","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Drug Delivery and Therapeutics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22270/jddt.v14i7.6702","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
A validated RP-HPLC/UV method was developed to estimate the selpercatinib in capsules. The wavelength chosen for detection was at 248 nm. The RP-HPLC separation was carried out using a Hypersil ODS C18 column (250×4.6 mm, 5 μm) with a mobile phase consisting of 0.2% TFA (pH 6.5) and acetonitrile in a ratio of 70:30 v/v. The flow rate was set at 1 ml/min. The retention time for selpercatinib was determined to be 3.012 min. Linearity was detected within the concentration range of 2.5-15 μg/mL for selpercatinib. The approach has been confirmed to be linear, accurate, precise, robust, and has established limits of detection and quantitation. The established procedure was uncomplicated, cost-effective, and suitable for the routine analysis of selpercatinib in capsule dosage form.
Keywords: Selpercatinib, RP-HPLC and Validation.
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