Pub Date : 2024-07-15DOI: 10.22270/jddt.v14i7.6452
Ishimwe ALAIN PRUDENCE, Migabo Hiberte, Tuyishime Jean De Dieu, Nzeyimana Godefroid
Background: The antimicrobial activities of medicinal plants have long been exploited by scientific community against the diseases causing microorganisms. Routine use of antibiotic agents developed microbes as drug resistant organisms making it as a critical issue in case of treatments. So the detection of new natural compounds with antimicrobial activity is considered to be important. Although hundreds of plant species have been said to have antimicrobial properties, the vast majority have not been adequately evaluated. The present research works focus to develop new therapeutic agents or drug from selected medicinal plant of Evolvulus alsinoides against HIV Secondary pathogenic organisms, based on the review of literature, problems associated with treatment of pathogenic organisms and drug resistant patterns of the pathogenic organisms. Results: The isolated bacterial cultures from secondary infection of HIV patient’s samples were identified as Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa based on their Morphology, Staining, Motility and Biochemical test. The isolated fungal cultures were identified as, Aspergillus niger, Penicillium sp, Mucor sp, and Candida albicans, based on their growth and LCB staining. Evolvulus alsinoides has shown to have several compounds that play a great function in its antimicrobial activity. Conclusion: There is a need to develop alternative agents for the control of pathogenic bacterial and fungal diseases in HIV infected persons. There is a good reason to support that the secondary metabolites of plants have evolved to protect them from attack by microbial pathogens. Hence, products from plants have great potential as sources of novel bioactive compounds for controlling pathogenic microorganisms. The treatment of various infectious diseases by the support of traditional usage of herbal medicine indicates the therapeutic potential of Evolvulus alsinoides by its antimicrobial activity. Further clinical studies are required to validate the efficacy of this medicinal plant against various pathogens and various resistant strains of bacteria. Keywords: Evolvulus alsinoides, HIV Secondary Pathogens, Solvent extracts, antimicrobial activity, Phytochemical Analysis.
背景：长期以来，科学界一直在利用药用植物的抗菌活性来对付致病微生物。抗生素制剂的常规使用使微生物产生了耐药性，成为治疗过程中的一个关键问题。因此，检测具有抗菌活性的新天然化合物被认为是非常重要的。虽然据说有数百种植物具有抗菌特性，但绝大多数植物尚未得到充分评估。本研究工作的重点是根据文献综述、与病原生物治疗相关的问题以及病原生物的耐药模式，从精选的药用植物 Evolvulus alsinoides 中开发新的治疗剂或药物，以抗击 HIV 二级病原生物。结果：根据形态学、染色、运动性和生化测试，从艾滋病毒继发感染患者样本中分离出的细菌培养物被鉴定为大肠埃希菌、金黄色葡萄球菌、肺炎克雷伯菌和铜绿假单胞菌。根据黑曲霉、青霉、粘孢子菌和白色念珠菌的生长情况和 LCB 染色结果，确定分离出的真菌培养物为黑曲霉、青霉、粘孢子菌和白色念珠菌。结果表明，Evolvulus alsinoides 的多种化合物在其抗菌活性中发挥了重要作用。结论：有必要开发替代药物，以控制艾滋病毒感染者的致病细菌和真菌疾病。有充分的理由证明，植物的次级代谢产物在进化过程中保护了植物免受微生物病原体的侵袭。因此，植物产品作为控制病原微生物的新型生物活性化合物来源具有巨大潜力。通过使用传统草药治疗各种传染性疾病表明，白花前胡具有抗菌治疗潜力。要验证这种药用植物对各种病原体和各种耐药菌株的疗效，还需要进一步的临床研究。关键词：Evolvulus alsinoides白花蛇舌草 HIV 继发性病原体 溶剂提取物 抗菌活性 植物化学分析
{"title":"Antimicrobial Activity and Phytochemical analysis of Evolvulus alsinoides against HIV Secondary Pathogens","authors":"Ishimwe ALAIN PRUDENCE, Migabo Hiberte, Tuyishime Jean De Dieu, Nzeyimana Godefroid","doi":"10.22270/jddt.v14i7.6452","DOIUrl":"https://doi.org/10.22270/jddt.v14i7.6452","url":null,"abstract":"Background: The antimicrobial activities of medicinal plants have long been exploited by scientific community against the diseases causing microorganisms. Routine use of antibiotic agents developed microbes as drug resistant organisms making it as a critical issue in case of treatments. So the detection of new natural compounds with antimicrobial activity is considered to be important. Although hundreds of plant species have been said to have antimicrobial properties, the vast majority have not been adequately evaluated. The present research works focus to develop new therapeutic agents or drug from selected medicinal plant of Evolvulus alsinoides against HIV Secondary pathogenic organisms, based on the review of literature, problems associated with treatment of pathogenic organisms and drug resistant patterns of the pathogenic organisms. \u0000Results: The isolated bacterial cultures from secondary infection of HIV patient’s samples were identified as Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa based on their Morphology, Staining, Motility and Biochemical test. The isolated fungal cultures were identified as, Aspergillus niger, Penicillium sp, Mucor sp, and Candida albicans, based on their growth and LCB staining. Evolvulus alsinoides has shown to have several compounds that play a great function in its antimicrobial activity. \u0000Conclusion: There is a need to develop alternative agents for the control of pathogenic bacterial and fungal diseases in HIV infected persons. There is a good reason to support that the secondary metabolites of plants have evolved to protect them from attack by microbial pathogens. Hence, products from plants have great potential as sources of novel bioactive compounds for controlling pathogenic microorganisms. The treatment of various infectious diseases by the support of traditional usage of herbal medicine indicates the therapeutic potential of Evolvulus alsinoides by its antimicrobial activity. Further clinical studies are required to validate the efficacy of this medicinal plant against various pathogens and various resistant strains of bacteria. \u0000Keywords: Evolvulus alsinoides, HIV Secondary Pathogens, Solvent extracts, antimicrobial activity, Phytochemical Analysis.","PeriodicalId":15622,"journal":{"name":"Journal of Drug Delivery and Therapeutics","volume":" 33","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141832808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-15DOI: 10.22270/jddt.v14i7.6702
Paka Ramya, Medidi Srinivas, Bula Udaya Kumari
A validated RP-HPLC/UV method was developed to estimate the selpercatinib in capsules. The wavelength chosen for detection was at 248 nm. The RP-HPLC separation was carried out using a Hypersil ODS C18 column (250×4.6 mm, 5 μm) with a mobile phase consisting of 0.2% TFA (pH 6.5) and acetonitrile in a ratio of 70:30 v/v. The flow rate was set at 1 ml/min. The retention time for selpercatinib was determined to be 3.012 min. Linearity was detected within the concentration range of 2.5-15 μg/mL for selpercatinib. The approach has been confirmed to be linear, accurate, precise, robust, and has established limits of detection and quantitation. The established procedure was uncomplicated, cost-effective, and suitable for the routine analysis of selpercatinib in capsule dosage form. Keywords: Selpercatinib, RP-HPLC and Validation.
{"title":"Development of a Validated RP-HPLC/UV Method for the Quantitative Determination of Tyrosine Kinase RET Inhibitor: Selpercatinib in Capsule Formulation","authors":"Paka Ramya, Medidi Srinivas, Bula Udaya Kumari","doi":"10.22270/jddt.v14i7.6702","DOIUrl":"https://doi.org/10.22270/jddt.v14i7.6702","url":null,"abstract":"A validated RP-HPLC/UV method was developed to estimate the selpercatinib in capsules. The wavelength chosen for detection was at 248 nm. The RP-HPLC separation was carried out using a Hypersil ODS C18 column (250×4.6 mm, 5 μm) with a mobile phase consisting of 0.2% TFA (pH 6.5) and acetonitrile in a ratio of 70:30 v/v. The flow rate was set at 1 ml/min. The retention time for selpercatinib was determined to be 3.012 min. Linearity was detected within the concentration range of 2.5-15 μg/mL for selpercatinib. The approach has been confirmed to be linear, accurate, precise, robust, and has established limits of detection and quantitation. The established procedure was uncomplicated, cost-effective, and suitable for the routine analysis of selpercatinib in capsule dosage form. \u0000Keywords: Selpercatinib, RP-HPLC and Validation.","PeriodicalId":15622,"journal":{"name":"Journal of Drug Delivery and Therapeutics","volume":" 25","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141832999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-15DOI: 10.22270/jddt.v14i7.6644
A. Mandal
Patients having cancer, infectious and other diseases suffer from drug resistance and systemic side effects owing to the conventional chemotherapeutics’ insolubility, toxicity, non-specificity, low therapeutic indices, and several limitations against biological barriers. To overcome these obstacles, nanotechnology-based metallic platinum nanoparticles (PtNPs) have attracted attention for targeted drug delivery and sustained release against the diseases for the cellular killing as antimicrobial and anticancer agents. PtNPs-based therapeutic systems have been utilized to avail their higher therapeutic efficacies with low concentrations against various diseases due to their suitable physico-chemical features such as shape, size, high surface to volume ratio, favorable bio-stability, easy membrane penetration, and easy surface functionalizations with cargos, ligands, peptides, antibodies and polymers for the targeted and controlled therapy against diseases. PtNPs may also be conjugated with other metals with drugs as suitable carriers for their chemo, photothermal / photoacoustic / magnetic therapies against tumors. This review demonstrates mainly the synthesis, functionalization, mechanism of action, biomedical application and toxicity of PtNPs as suitable nanomedicinal delivery system against diseases. Keywords: Diseases; Platinum nanoparticles; Delivery system; Therapeutic efficacies
{"title":"Platinum nanoparticles as delivery system in combating various diseases","authors":"A. Mandal","doi":"10.22270/jddt.v14i7.6644","DOIUrl":"https://doi.org/10.22270/jddt.v14i7.6644","url":null,"abstract":"Patients having cancer, infectious and other diseases suffer from drug resistance and systemic side effects owing to the conventional chemotherapeutics’ insolubility, toxicity, non-specificity, low therapeutic indices, and several limitations against biological barriers. To overcome these obstacles, nanotechnology-based metallic platinum nanoparticles (PtNPs) have attracted attention for targeted drug delivery and sustained release against the diseases for the cellular killing as antimicrobial and anticancer agents. PtNPs-based therapeutic systems have been utilized to avail their higher therapeutic efficacies with low concentrations against various diseases due to their suitable physico-chemical features such as shape, size, high surface to volume ratio, favorable bio-stability, easy membrane penetration, and easy surface functionalizations with cargos, ligands, peptides, antibodies and polymers for the targeted and controlled therapy against diseases. PtNPs may also be conjugated with other metals with drugs as suitable carriers for their chemo, photothermal / photoacoustic / magnetic therapies against tumors. This review demonstrates mainly the synthesis, functionalization, mechanism of action, biomedical application and toxicity of PtNPs as suitable nanomedicinal delivery system against diseases. \u0000Keywords: Diseases; Platinum nanoparticles; Delivery system; Therapeutic efficacies ","PeriodicalId":15622,"journal":{"name":"Journal of Drug Delivery and Therapeutics","volume":" 104","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141833722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-15DOI: 10.22270/jddt.v14i7.6673
Samikshya Sharma, San Tshering Lepcha, Bidhya Sharma, Sonam Bhutia
In recent years, the global cosmetics industry has witnessed a notable shift in consumer preferences towards natural and organic products, leading to an increased demand for herbal cosmetics derived from plant-based ingredients. This research survey presents a comparative analysis of herbal and synthetic cosmetics, exploring distinctions in formulation, efficacy, safety, and environmental impact. Herbal cosmetics incorporate natural elements like plant extracts, essential oils, herbs, and fruits, while synthetic counterparts are developed using chemical compounds in laboratories to replicate specific properties. The main objective is to find out the preferences between herbal and synthetic cosmetics among individuals in Sikkim. The methodology adopted for mini survey was pre-prepared questionnaires were developed by using Google forms and shared through the online platforms such as Mail, Whatapps and facebook. An online survey conducted in different districts of Sikkim revealed that 42% of respondents favoured herbal cosmetics, while 31% preferred synthetic products and 23% had no specific preference. Factors influencing those leaning toward synthetic cosmetics included perceived effectiveness, availability, and affordability. Despite the clear interest and preference for herbal cosmetics, the survey also highlighted limited choice and availability in comparison to synthetic alternatives. This suggests a substantial opportunity for growth in the herbal cosmetic industry, emphasizing the need for increased accessibility to meet the growing demand in Sikkim and beyond. Keywords: Knowledge of Herbal, An Online Survey, Herbal Cosmetics, Acceptability, Usability, Sikkimese People
{"title":"An Online Survey on Usability, Acceptability, Attitude and Knowledge of Herbal and Synthetic Cosmetic Among Sikkimese Population","authors":"Samikshya Sharma, San Tshering Lepcha, Bidhya Sharma, Sonam Bhutia","doi":"10.22270/jddt.v14i7.6673","DOIUrl":"https://doi.org/10.22270/jddt.v14i7.6673","url":null,"abstract":"In recent years, the global cosmetics industry has witnessed a notable shift in consumer preferences towards natural and organic products, leading to an increased demand for herbal cosmetics derived from plant-based ingredients. This research survey presents a comparative analysis of herbal and synthetic cosmetics, exploring distinctions in formulation, efficacy, safety, and environmental impact. Herbal cosmetics incorporate natural elements like plant extracts, essential oils, herbs, and fruits, while synthetic counterparts are developed using chemical compounds in laboratories to replicate specific properties. The main objective is to find out the preferences between herbal and synthetic cosmetics among individuals in Sikkim. The methodology adopted for mini survey was pre-prepared questionnaires were developed by using Google forms and shared through the online platforms such as Mail, Whatapps and facebook. An online survey conducted in different districts of Sikkim revealed that 42% of respondents favoured herbal cosmetics, while 31% preferred synthetic products and 23% had no specific preference. Factors influencing those leaning toward synthetic cosmetics included perceived effectiveness, availability, and affordability. Despite the clear interest and preference for herbal cosmetics, the survey also highlighted limited choice and availability in comparison to synthetic alternatives. This suggests a substantial opportunity for growth in the herbal cosmetic industry, emphasizing the need for increased accessibility to meet the growing demand in Sikkim and beyond. \u0000Keywords: Knowledge of Herbal, An Online Survey, Herbal Cosmetics, Acceptability, Usability, Sikkimese People","PeriodicalId":15622,"journal":{"name":"Journal of Drug Delivery and Therapeutics","volume":" 43","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141832896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-15DOI: 10.22270/jddt.v14i7.6690
Deepa Shrivastava, Surabhi Saksena
The most prevalent digestive condition in clinical practice is peptic ulcer. It should be regarded as a superior choice for the treatment of peptic ulcer given the numerous side effects of contemporary medicine, the initial acquisition of fewer side effects, and the therapy of indigenous substances. Throughout most of India's deciduous woods, Buchanania lanzan (B. lanzan, Chironji, Achar, Anacardiaceae) is a significant non-wood tree species. B. lanzan is a commonly used plant that has been used for many years in traditional medicine to treat a variety of illnesses. Thus, the goal of the current investigation was to assess the hydroalcoholic extract of B. lanzan leaves antiulcer properties in rats. The established test procedure described in the literature was used to determine the quantitative analysis of total phenolics and flavonoids as well as the qualitative analysis of different phytochemical constituents. Rats with pylorus-ligated peptic ulcer models were used to test the hydroalcoholic extract of B. lanzan leaves' in vivo anti-ulcer activity. The volume and pH of stomach fluid, free acidity, total acidity, ulcer index, and percent inhibition of ulcer index were among the outcome indicators that varied based on the model. An initial phytochemical examination indicated the existence of flavonoids, phenols, glycosides, alkaloids, and tannins. The leaves hydroalcoholic extract had a total phenolic content of 32.5 mg/gm, with flavonoids coming in second at 27.60 mg/gm. In rats with pylorus ligation-induced ulcer models, additional hydroalcoholic extract at 200 and 400 mg/kg/p.o. significantly (p˂0.01) decreased the gastric volume, pH, ulcer number, ulcer index, free acidity, and total acidity. The results of this investigation verified that B. lanzan extract, because of one or more of its secondary metabolites, possesses pharmacologic activity that is anti-ulcer. This study thus supports the use of it in Indian traditional medicine as an anti-ulcer agent. It is necessary to conduct additional research to isolate particular phytochemicals and clarify their mechanisms of action. Keywords: Buchanania lanzan, Phytochemical constituents, Antiulcer, Pylorus ligation, Peptic ulcer
{"title":"Qualitative and Quantitative Determination of Secondary Metabolites and Evaluation of Antiulcer Activity of Hydroalcoholic Extract of Buchanania lanzan Leaf Extract","authors":"Deepa Shrivastava, Surabhi Saksena","doi":"10.22270/jddt.v14i7.6690","DOIUrl":"https://doi.org/10.22270/jddt.v14i7.6690","url":null,"abstract":"The most prevalent digestive condition in clinical practice is peptic ulcer. It should be regarded as a superior choice for the treatment of peptic ulcer given the numerous side effects of contemporary medicine, the initial acquisition of fewer side effects, and the therapy of indigenous substances. Throughout most of India's deciduous woods, Buchanania lanzan (B. lanzan, Chironji, Achar, Anacardiaceae) is a significant non-wood tree species. B. lanzan is a commonly used plant that has been used for many years in traditional medicine to treat a variety of illnesses. Thus, the goal of the current investigation was to assess the hydroalcoholic extract of B. lanzan leaves antiulcer properties in rats. The established test procedure described in the literature was used to determine the quantitative analysis of total phenolics and flavonoids as well as the qualitative analysis of different phytochemical constituents. Rats with pylorus-ligated peptic ulcer models were used to test the hydroalcoholic extract of B. lanzan leaves' in vivo anti-ulcer activity. The volume and pH of stomach fluid, free acidity, total acidity, ulcer index, and percent inhibition of ulcer index were among the outcome indicators that varied based on the model. An initial phytochemical examination indicated the existence of flavonoids, phenols, glycosides, alkaloids, and tannins. The leaves hydroalcoholic extract had a total phenolic content of 32.5 mg/gm, with flavonoids coming in second at 27.60 mg/gm. In rats with pylorus ligation-induced ulcer models, additional hydroalcoholic extract at 200 and 400 mg/kg/p.o. significantly (p˂0.01) decreased the gastric volume, pH, ulcer number, ulcer index, free acidity, and total acidity. The results of this investigation verified that B. lanzan extract, because of one or more of its secondary metabolites, possesses pharmacologic activity that is anti-ulcer. This study thus supports the use of it in Indian traditional medicine as an anti-ulcer agent. It is necessary to conduct additional research to isolate particular phytochemicals and clarify their mechanisms of action. \u0000Keywords: Buchanania lanzan, Phytochemical constituents, Antiulcer, Pylorus ligation, Peptic ulcer","PeriodicalId":15622,"journal":{"name":"Journal of Drug Delivery and Therapeutics","volume":" 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141833140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-15DOI: 10.22270/jddt.v14i7.6655
Saif Ali Siddiqui, Mohammad Shoaib, Asia Sultana
Background: Since ancient times, Unani physicians have controlled certain illnesses by altering the metabolic process using a variety of therapeutic approaches. Some treatment tenets have been promoted by Ibne-sina (Avicenna). Regimenal therapy is a fundamental approach to treatment that aims to eradicate sick matter, control excessive production, control its spread, and resolve it by specialised approaches, ultimately restoring humoral equilibrium. Hijama (Cupping) is one such operation. The Arabic word "Hijamat" (from the phrase “Hajm”) means "volume", but it is also used to refer to sucking. Additionally, it might indicate "to minimise", "to return to basic size", or "to reduce the volume". One of the most prevalent symptoms of Irq-al-nasa (Sciatica) is lower limb neuralgic pain. This illness has the potential to become persistent and chronic, with significant socioeconomic consequences. Its overall incidence is estimated to be between 13% and 40%. Objective: Mainstream therapists primarily treat Sciatica with medication, surgery, or occasionally both. There should be an efficient substitute for evidence-based medicine because of its negative impacts, high cost, and disease-centric approach to treatment. Extensive research has been conducted to investigate the potential benefits of this traditional treatment plan for the management of Sciatica. Keywords: Cupping, Irq-al-nasa, Sciatica, Hijama, Unani medicine
{"title":"Exploring Hijama (Cupping Therapy) for Sciatica Relief: An Integrative Review of Unani Practices","authors":"Saif Ali Siddiqui, Mohammad Shoaib, Asia Sultana","doi":"10.22270/jddt.v14i7.6655","DOIUrl":"https://doi.org/10.22270/jddt.v14i7.6655","url":null,"abstract":"Background: Since ancient times, Unani physicians have controlled certain illnesses by altering the metabolic process using a variety of therapeutic approaches. Some treatment tenets have been promoted by Ibne-sina (Avicenna). Regimenal therapy is a fundamental approach to treatment that aims to eradicate sick matter, control excessive production, control its spread, and resolve it by specialised approaches, ultimately restoring humoral equilibrium. Hijama (Cupping) is one such operation. The Arabic word \"Hijamat\" (from the phrase “Hajm”) means \"volume\", but it is also used to refer to sucking. Additionally, it might indicate \"to minimise\", \"to return to basic size\", or \"to reduce the volume\". One of the most prevalent symptoms of Irq-al-nasa (Sciatica) is lower limb neuralgic pain. This illness has the potential to become persistent and chronic, with significant socioeconomic consequences. Its overall incidence is estimated to be between 13% and 40%. Objective: Mainstream therapists primarily treat Sciatica with medication, surgery, or occasionally both. There should be an efficient substitute for evidence-based medicine because of its negative impacts, high cost, and disease-centric approach to treatment. Extensive research has been conducted to investigate the potential benefits of this traditional treatment plan for the management of Sciatica. \u0000Keywords: Cupping, Irq-al-nasa, Sciatica, Hijama, Unani medicine","PeriodicalId":15622,"journal":{"name":"Journal of Drug Delivery and Therapeutics","volume":" 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141833257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-15DOI: 10.22270/jddt.v14i7.6674
Abhijit Pednekar, Amit Gupta, S. Warrier
Objective: To assess the pharmacokinetics and bioequivalence of Dapagliflozin 10mg + Vildagliptin Sustained Release (SR) 100mg + Metformin SR 1000mg fixed dose combination (FDC) tablets with DAPAMAC V 10 (Dapagliflozin 10mg + Vildagliptin SR 100mg tablets) and Glycomet 1g (Metformin SR 1000 mg tablets) in healthy adult male subjects under fasting conditions. Material and Methods: This was an open-label, balanced, randomized, two-treatment, two-period, two-sequence, single-dose, crossover, oral bioequivalence study. Volunteers were randomized to receive either a test product or a reference product under the fasting condition with a seven-day washout period. The pharmacokinetics parameters evaluated were maximum plasma concentration (Cmax), the area under the curve (AUC) at time t (AUC0-t), and the total area under the curve (AUC0-∞). Adverse events were also assessed as safety endpoints. Results: Twenty-four healthy adult male subjects were randomized, and 24 completed the study. The mean values for Cmax, AUC0-t, and AUC0-∞ were almost identical for test and reference products. The 90% confidence intervals of the ratios of adjusted geometric means for the pharmacokinetic parameters, i.e. Cmax, AUC0-t, and AUC0-∞ of the test product, were within the predefined bioequivalence limits of 80.00 to 125.00%. No adverse events or serious adverse events or deaths were reported during the study. All treatments were well tolerated. Conclusion: The test formulation, Dapagliflozin 10mg + Vildagliptin SR 100mg + Metformin SR 1000mg FDC, were bioequivalent to DAPAMAC V 10 (Dapagliflozin 10mg + Vildagliptin SR 100mg tablets) and Glycomet 1g (Metformin 1000mg SR tablets) in healthy adult male subjects under fasting conditions. Keywords: Bioequivalence, Fixed-Dose Combination, Dapagliflozin, Metformin, Sustained Release, Vildagliptin, AUC, Cmax
{"title":"The MeViDa Study: Bioequivalence Study of FDC of Dapagliflozin, Vildagliptin SR and Metformin SR in Healthy Indian Volunteers: A Randomized, Open-Label, Crossover Study","authors":"Abhijit Pednekar, Amit Gupta, S. Warrier","doi":"10.22270/jddt.v14i7.6674","DOIUrl":"https://doi.org/10.22270/jddt.v14i7.6674","url":null,"abstract":"Objective: To assess the pharmacokinetics and bioequivalence of Dapagliflozin 10mg + Vildagliptin Sustained Release (SR) 100mg + Metformin SR 1000mg fixed dose combination (FDC) tablets with DAPAMAC V 10 (Dapagliflozin 10mg + Vildagliptin SR 100mg tablets) and Glycomet 1g (Metformin SR 1000 mg tablets) in healthy adult male subjects under fasting conditions. \u0000Material and Methods: This was an open-label, balanced, randomized, two-treatment, two-period, two-sequence, single-dose, crossover, oral bioequivalence study. Volunteers were randomized to receive either a test product or a reference product under the fasting condition with a seven-day washout period. The pharmacokinetics parameters evaluated were maximum plasma concentration (Cmax), the area under the curve (AUC) at time t (AUC0-t), and the total area under the curve (AUC0-∞). Adverse events were also assessed as safety endpoints. \u0000Results: Twenty-four healthy adult male subjects were randomized, and 24 completed the study. The mean values for Cmax, AUC0-t, and AUC0-∞ were almost identical for test and reference products. The 90% confidence intervals of the ratios of adjusted geometric means for the pharmacokinetic parameters, i.e. Cmax, AUC0-t, and AUC0-∞ of the test product, were within the predefined bioequivalence limits of 80.00 to 125.00%. No adverse events or serious adverse events or deaths were reported during the study. All treatments were well tolerated. \u0000Conclusion: The test formulation, Dapagliflozin 10mg + Vildagliptin SR 100mg + Metformin SR 1000mg FDC, were bioequivalent to DAPAMAC V 10 (Dapagliflozin 10mg + Vildagliptin SR 100mg tablets) and Glycomet 1g (Metformin 1000mg SR tablets) in healthy adult male subjects under fasting conditions. \u0000Keywords: Bioequivalence, Fixed-Dose Combination, Dapagliflozin, Metformin, Sustained Release, Vildagliptin, AUC, Cmax","PeriodicalId":15622,"journal":{"name":"Journal of Drug Delivery and Therapeutics","volume":" 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141833118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-15DOI: 10.22270/jddt.v14i7.6683
Younous Yaya Charfadine, Rodolphe Koularambaye, Sara Nathalie Edjenguèlè Béboy, Joseph Rodrigue Fifen Ngapout, Yannick Sani Jignoua, D. Dzeufiet, P. Moundipa
Background: Androgen deficiency is the most common disorder of reproductive function and can lead to male sexual disorders. Objective: This study was designed to evaluate the androgenic effects of Pycnanthus angolensis (Welw.) Warb in hemicastrated rats. Materials and methods: Forty-two male rats were divided into 6 groups of 7 rats each, including a group of uncastrated rats that received distilled water (10 ml/kg); a group of castrated rats that received 10 ml/kg of distilled water; a group of castrated rats that received testosterone enanthate (3 mg/kg BW) per week intramuscularly; and 3 groups of castrated rats that received 43, 86 and 172 mg/kg of the aqueous extract of Pycnanthus angolensis, respectively. After 14 days of oral treatment, the rats were killed by decapitation. The blood was collected and the androgen-dependent organs were collected for histological sectioning, and biochemical analysis. The tail of the epididymis was used to assess sperm quality. Results: Treatment with the aqueous extract at doses of 43 and 86 mg/kg, significantly improved the sexual behavior of castrated rats, with increases of 25.92% and 22.74% intromissions frequency, and 67.06% and 56.46% mount frequency, compared to those in the castrated rats which did not receive any treatment. The extract also enhanced sperm quality in castrated rats. Both doses also significantly increased serum testosterone levels with rates of 45.07% and 49.00%, respectively; compared to those in the negative control group. Conclusion: In view of the aforesaid results, Pycnanthus angolensis (Welw.) Warb could be considered as a promising natural agent in hypogonadism management. Keywords: Androgen, hypogonadism, castrated male rats, unilateral castration, Pycnanthus angolensis (Welw.) Warb
{"title":"Androgenic effects of the aqueous extract of Pycnanthus angolensis (Welw.) Warb. (Myristicaceae) wood in hemicastrated male Wistar rats","authors":"Younous Yaya Charfadine, Rodolphe Koularambaye, Sara Nathalie Edjenguèlè Béboy, Joseph Rodrigue Fifen Ngapout, Yannick Sani Jignoua, D. Dzeufiet, P. Moundipa","doi":"10.22270/jddt.v14i7.6683","DOIUrl":"https://doi.org/10.22270/jddt.v14i7.6683","url":null,"abstract":"Background: Androgen deficiency is the most common disorder of reproductive function and can lead to male sexual disorders. \u0000Objective: This study was designed to evaluate the androgenic effects of Pycnanthus angolensis (Welw.) Warb in hemicastrated rats. \u0000Materials and methods: Forty-two male rats were divided into 6 groups of 7 rats each, including a group of uncastrated rats that received distilled water (10 ml/kg); a group of castrated rats that received 10 ml/kg of distilled water; a group of castrated rats that received testosterone enanthate (3 mg/kg BW) per week intramuscularly; and 3 groups of castrated rats that received 43, 86 and 172 mg/kg of the aqueous extract of Pycnanthus angolensis, respectively. After 14 days of oral treatment, the rats were killed by decapitation. The blood was collected and the androgen-dependent organs were collected for histological sectioning, and biochemical analysis. The tail of the epididymis was used to assess sperm quality. \u0000Results: Treatment with the aqueous extract at doses of 43 and 86 mg/kg, significantly improved the sexual behavior of castrated rats, with increases of 25.92% and 22.74% intromissions frequency, and 67.06% and 56.46% mount frequency, compared to those in the castrated rats which did not receive any treatment. The extract also enhanced sperm quality in castrated rats. Both doses also significantly increased serum testosterone levels with rates of 45.07% and 49.00%, respectively; compared to those in the negative control group. \u0000Conclusion: In view of the aforesaid results, Pycnanthus angolensis (Welw.) Warb could be considered as a promising natural agent in hypogonadism management. \u0000Keywords: Androgen, hypogonadism, castrated male rats, unilateral castration, Pycnanthus angolensis (Welw.) Warb","PeriodicalId":15622,"journal":{"name":"Journal of Drug Delivery and Therapeutics","volume":" 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141833124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-15DOI: 10.22270/jddt.v14i7.6668
King Akpofure Nelson Esievo, Emmanuel Oluwadare Balogun, Kingsley Oghenerukevwe Esievo, Lauretta Oghenekevwe Esievo, Edith Monica Esievo, Dahiru Sani, John Wassagwa, E. O. Uyovbisere, Emmanuel Tamajong Mumah
Objectives: The study aimed to identify the antidiabetic compounds purified from stem bark of Anogeissus leiocarpus and propose the mechanisms of action. Design: Anogeissus leiocarpus stem bark was purified through ethyl acetate and n-hexane with minor exceptions. For very clear separation, cold acetone was added to trigger the precipitation. The precipitate was dissolved with a mixture of DCM:methanol (9:1), adsorbed it to silica gel (5 g), evaporated to free flowing powder and fractionated it over silica gel (50 g) to realize 40 fractions. The gummy fractions were ignored. The light brown powder which possessed antidiabetic effect was selected for Nuclear Magnetic Resonance for structural elucidation. Nuclear Magnetic Resonance (NMR) and Determination of Structure of Purified Compound: After column chromatography and TLC processes, along with the cold acetone, to the point of crystallization, the purified compounds, the light brown powder, were presented to NMR (Bruker Avance III, Spectrometer frequency 400 MHz; solvents DMSO-do, CDCl3, Acetone-d6. Institute of Chemistry, Strathclyde University Glasgow UK.) for determination of compound structures and analyses. Results: The NMR spectra and analyses revealed the existence of Betulinic acid and Trimethoxyellagic acid. Conclusion: We show that Betulinic acid and Trimethoxyellagic acid are potent antidiabetic compounds in the stem bark extract of A. leiocarpus. Keywords: Anogeissus leiocarpus, Betulinic Acid, Trimethoxyellagic Acid, Antidiabetic agent
{"title":"Identification of Betulinic Acid and Trimethoxyellagic Acid as the Antidiabetic Compounds in Anogeissus leiocarpus Stem Bark Purified Extract","authors":"King Akpofure Nelson Esievo, Emmanuel Oluwadare Balogun, Kingsley Oghenerukevwe Esievo, Lauretta Oghenekevwe Esievo, Edith Monica Esievo, Dahiru Sani, John Wassagwa, E. O. Uyovbisere, Emmanuel Tamajong Mumah","doi":"10.22270/jddt.v14i7.6668","DOIUrl":"https://doi.org/10.22270/jddt.v14i7.6668","url":null,"abstract":"Objectives: The study aimed to identify the antidiabetic compounds purified from stem bark of Anogeissus leiocarpus and propose the mechanisms of action. \u0000Design: Anogeissus leiocarpus stem bark was purified through ethyl acetate and n-hexane with minor exceptions. For very clear separation, cold acetone was added to trigger the precipitation. The precipitate was dissolved with a mixture of DCM:methanol (9:1), adsorbed it to silica gel (5 g), evaporated to free flowing powder and fractionated it over silica gel (50 g) to realize 40 fractions. The gummy fractions were ignored. The light brown powder which possessed antidiabetic effect was selected for Nuclear Magnetic Resonance for structural elucidation. \u0000Nuclear Magnetic Resonance (NMR) and Determination of Structure of Purified Compound: After column chromatography and TLC processes, along with the cold acetone, to the point of crystallization, the purified compounds, the light brown powder, were presented to NMR (Bruker Avance III, Spectrometer frequency 400 MHz; solvents DMSO-do, CDCl3, Acetone-d6. Institute of Chemistry, Strathclyde University Glasgow UK.) for determination of compound structures and analyses. \u0000Results: The NMR spectra and analyses revealed the existence of Betulinic acid and Trimethoxyellagic acid. \u0000Conclusion: We show that Betulinic acid and Trimethoxyellagic acid are potent antidiabetic compounds in the stem bark extract of A. leiocarpus. \u0000Keywords: Anogeissus leiocarpus, Betulinic Acid, Trimethoxyellagic Acid, Antidiabetic agent","PeriodicalId":15622,"journal":{"name":"Journal of Drug Delivery and Therapeutics","volume":" 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141833484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
UV Spectrophotometric method has been developed to determine Gliclazide in bulk pure and tablet forms. It is a simple, accurate, reproducible, rapid and less time-consuming method. The maximum wavelength of the drug was found to be 232nm. Beer Lamberts’ law was obeyed in the concentration range of 2-20 µg/ml. (LOD)The limit of detection and limit of qualification (LOQ) was found to be 0.16 µg/ml and 0.50 µg/ml from this method per cent recovery of the drug was found to be 99.30% which indicates no interaction of the excipients. This method was found accurate, simple, precise and rapid for determination of tablet dosage form. Keywords: validation, Gliclazide, tablets, UV Spectroscopy, LOD, LOQ
{"title":"Method development and validation for estimation of Gliclazide in bulk and tablet form by UV Spectrophotometer","authors":"Ravindra Hanwate, Bhupendra Singh, Nilesh Khairnar, Sushmita Chavan","doi":"10.22270/jddt.v14i7.6675","DOIUrl":"https://doi.org/10.22270/jddt.v14i7.6675","url":null,"abstract":"UV Spectrophotometric method has been developed to determine Gliclazide in bulk pure and tablet forms. It is a simple, accurate, reproducible, rapid and less time-consuming method. The maximum wavelength of the drug was found to be 232nm. Beer Lamberts’ law was obeyed in the concentration range of 2-20 µg/ml. (LOD)The limit of detection and limit of qualification (LOQ) was found to be 0.16 µg/ml and 0.50 µg/ml from this method per cent recovery of the drug was found to be 99.30% which indicates no interaction of the excipients. This method was found accurate, simple, precise and rapid for determination of tablet dosage form. \u0000Keywords: validation, Gliclazide, tablets, UV Spectroscopy, LOD, LOQ","PeriodicalId":15622,"journal":{"name":"Journal of Drug Delivery and Therapeutics","volume":" 21","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141833105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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