Sphingomonas sp. Hbc-6 alters Arabidopsis metabolites to improve plant growth and drought resistance by manipulating the microbiome

IF 6.1 1区 生物学 Q1 MICROBIOLOGY Microbiological research Pub Date : 2024-07-18 DOI:10.1016/j.micres.2024.127852
Fang Wang , Mingyue Jia , Kun Li , Yafang Cui , Lizhe An , Hongmei Sheng
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Abstract

Drought significantly affects crop productivity and poses a considerable threat to agricultural ecosystems. Plant growth-promoting bacteria (PGPB) and plant microbiome play important roles in improving drought resistance and plant performance. However, the response of the rhizosphere microbiota to PGPB during the development of plants and the interaction between inoculum, microbiota, and plants under drought stress remain to be explored. In the present study, we used culturomic, microbiomic, and metabonomic analyses to uncover the mechanisms by which Sphingomonas sp. Hbc-6, a PGPB, promotes Arabidopsis growth and enhances drought resistance. We found that the rhizosphere microbiome assembly was interactively influenced by developmental stage, Hbc-6, and drought; the bacterial composition exhibited three patterns of shifts with developmental stage: resilience, increase, and decrease. Drought diminished microbial diversity and richness, whereas Hbc-6 increased microbial diversity and helped plants recruit specific beneficial bacterial taxa at each developmental stage, particularly during the bolting stage. Some microorganisms enriched by Hbc-6 had the potential to promote carbon and nitrogen cycling processes, and 86.79 % of the isolated strains exhibited PGP characteristics (for example Pseudomonas sp. TA9). They jointly regulated plant physiological metabolism (i.e., upregulated drought resistant-facilitating substances and reduced harmful substances), thereby stimulating the growth of Arabidopsis and increasing plant biomass under drought stress conditions. Collectively, these results indicate that Hbc-6 mediates plant growth and drought resistance by affecting the microbiome. The study thus provides novel insights and strain resources for drought-resistant, high-yielding crop cultivation and breeding.

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拟南芥鞘氨单胞菌 Hbc-6 通过操纵微生物组改变拟南芥代谢物,从而改善植物生长和抗旱性
干旱严重影响作物产量,并对农业生态系统构成巨大威胁。植物生长促进菌(PGPB)和植物微生物群在提高抗旱性和植物表现方面发挥着重要作用。然而,植物生长过程中根瘤微生物群对 PGPB 的反应以及干旱胁迫下接种物、微生物群和植物之间的相互作用仍有待探索。在本研究中,我们利用培养物组学、微生物组学和代谢组学分析揭示了拟南芥根瘤单胞菌(Sphingomonas sp. Hbc-6)促进拟南芥生长和增强抗旱性的机制。我们发现,根瘤微生物组的组合受到发育阶段、Hbc-6和干旱的交互影响;细菌组成随着发育阶段的变化呈现出三种模式:恢复力、增加和减少。干旱降低了微生物的多样性和丰富度,而 Hbc-6 增加了微生物的多样性,并帮助植物在每个发育阶段(尤其是萌芽阶段)招募特定的有益细菌类群。Hbc-6 富集的一些微生物具有促进碳和氮循环过程的潜力,86.79% 的分离菌株表现出 PGP 特性(例如假单胞菌 TA9)。它们共同调节植物生理代谢(即上调抗旱促进物质和减少有害物质),从而在干旱胁迫条件下刺激拟南芥生长并增加植物生物量。总之,这些结果表明,Hbc-6 通过影响微生物组介导植物生长和抗旱。因此,该研究为抗旱高产作物栽培和育种提供了新的见解和菌株资源。
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来源期刊
Microbiological research
Microbiological research 生物-微生物学
CiteScore
10.90
自引率
6.00%
发文量
249
审稿时长
29 days
期刊介绍: Microbiological Research is devoted to publishing reports on prokaryotic and eukaryotic microorganisms such as yeasts, fungi, bacteria, archaea, and protozoa. Research on interactions between pathogenic microorganisms and their environment or hosts are also covered.
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