Preparation of starch-based green nanofiber mats for probiotic encapsulation by electrospinning

IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Journal of Food Science Pub Date : 2024-07-31 DOI:10.1111/1750-3841.17250
Yufei Wang, Haowei Lv, Chenxi Wang, Deyun He, Haibo Zhao, Enbo Xu, Zhengyu Jin, Zhengzong Wu, Pengfei Liu, Bo Cui
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Abstract

In this study, starch-based nanofiber mats were successfully prepared from aqueous solution by electrospinning and used for probiotic encapsulation for the first time. The physicochemical properties of the octenylsuccinated (OS) starch/poly(vinyl alcohol) (PVA) blend solutions were systematically investigated. Through Fourier transform infrared spectroscopy and X-ray diffraction spectra analysis, it was found that miscibility and hydrogen bonding interactions exist between OS starch and PVA molecules. Thermogravimetric analysis and derivative thermogravimetric analysis revealed that the produced nanofibers possess satisfactory thermal stability. Scanning electron microscopy images and diameter distribution histograms showed that continuous and defect-free nanofibers were obtained and along with the increase in the weight ratio of OS starch, the average diameter gradually decreased. In addition, it was confirmed that the probiotics were successfully encapsulated in nanofiber mats. The survival rates of Lactobacillus plantarum AB-1 and Lactobacillus rhamnosus GG encapsulated in nanofibers were as high as 94.63% and 92.42%, respectively, significantly higher than those of traditional freeze-drying. Moreover, compared to free cells, probiotics encapsulated in nanofiber mats retained better viability after 21 days of storage at 4 and 25°C, and showed remarkably higher survival rates after exposure to simulated gastric and intestinal fluid. This study showed that the developed nanofibers can be a promising encapsulation system for the protection of probiotics.

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利用电纺丝技术制备用于益生菌封装的淀粉基绿色纳米纤维毡。
本研究首次利用电纺丝技术从水溶液中成功制备了淀粉基纳米纤维毡,并将其用于益生菌封装。该研究系统地考察了辛烯基琥珀酰化(OS)淀粉/聚乙烯醇(PVA)共混溶液的理化性质。通过傅立叶变换红外光谱和 X 射线衍射光谱分析发现,OS 淀粉和 PVA 分子之间存在混溶和氢键作用。热重分析和衍生热重分析表明,制得的纳米纤维具有令人满意的热稳定性。扫描电子显微镜图像和直径分布直方图显示,制得的纳米纤维连续且无缺陷,随着 OS 淀粉重量比的增加,平均直径逐渐减小。此外,还证实益生菌被成功封装在纳米纤维毡中。封装在纳米纤维中的植物乳杆菌 AB-1 和鼠李糖乳杆菌 GG 的存活率分别高达 94.63% 和 92.42%,明显高于传统的冷冻干燥法。此外,与游离细胞相比,封装在纳米纤维毡中的益生菌在4℃和25℃条件下储存21天后仍能保持较好的存活率,在暴露于模拟胃液和肠液后的存活率也明显更高。这项研究表明,所开发的纳米纤维是一种很有前景的保护益生菌的封装系统。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Food Science
Journal of Food Science 工程技术-食品科技
CiteScore
7.10
自引率
2.60%
发文量
412
审稿时长
3.1 months
期刊介绍: The goal of the Journal of Food Science is to offer scientists, researchers, and other food professionals the opportunity to share knowledge of scientific advancements in the myriad disciplines affecting their work, through a respected peer-reviewed publication. The Journal of Food Science serves as an international forum for vital research and developments in food science. The range of topics covered in the journal include: -Concise Reviews and Hypotheses in Food Science -New Horizons in Food Research -Integrated Food Science -Food Chemistry -Food Engineering, Materials Science, and Nanotechnology -Food Microbiology and Safety -Sensory and Consumer Sciences -Health, Nutrition, and Food -Toxicology and Chemical Food Safety The Journal of Food Science publishes peer-reviewed articles that cover all aspects of food science, including safety and nutrition. Reviews should be 15 to 50 typewritten pages (including tables, figures, and references), should provide in-depth coverage of a narrowly defined topic, and should embody careful evaluation (weaknesses, strengths, explanation of discrepancies in results among similar studies) of all pertinent studies, so that insightful interpretations and conclusions can be presented. Hypothesis papers are especially appropriate in pioneering areas of research or important areas that are afflicted by scientific controversy.
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