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Announcing the 2024 Winner of the Kroger Outstanding Reviewer Award 宣布 2024 年克罗格杰出评论员奖得主。
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-03 DOI: 10.1111/1750-3841.17168
Rich Hartel

It is time to acknowledge our top reviewer for the past year. Annually, we recognize one reviewer for his/her contributions to the journal, in honor of Manfred Kroger. Dr. Kroger was a long-term editor for Comprehensive Reviews in Food Science and Food Safety (CRFSFS), well known for his diligence in making sure manuscripts were top notch. Much of the credit for the early success of CRFSFS goes to Dr. Kroger.

To select the Kroger award winner, the Associate Editors first nominate reviewers who have done exceptional review work for them in the past few years, using reviewer performance data to support their nominations. The Scientific Editors then vote as to which candidate should receive the award. The award is based on a number of factors; the number of reviews is important, but also the timeliness and quality of the reviews. The award is presented in person at our annual Reviewers’ Reception at IFT FIRST.

This year's Manfred Kroger award goes to Dr. Han-Seok Seo of the University of Arkansas, a leading expert in sensory analysis and an outstanding reviewer. He has completed 90 reviews in the last 5 years, averaging 11.6 days turnaround. Clearly, Dr. Seo deserves the 2023 Kroger award.

Seo: The scientific journal's organizational structure mirrors that of a soccer team. A typical soccer team's positions, that is, a goalkeeper, defenders, midfielders, and forwards, and their roles correspond, respectively, to an editor in chief, scientific/associate editors, peer reviewers, and authors. Like midfielders who help forwards score the balls or block the opposing team's attempts at scoring before reaching the defensive line, peer reviewers provide authors with helpful comments to improve the quality of their manuscripts. Moreover, peer reviewers play a crucial role in preventing inaccurate, low-quality, and misleading manuscripts from being published by carefully reviewing them. As the midfielder position is considered the engine of a soccer team, peer reviewers are a key component of the journal system. As a journal gains more well-trained, dedicated, and enthusiastic reviewers, its impact significantly grows, like how skilled, committed, and spirited players improve a soccer team's performance. Also, a combination of reviewers from diverse backgrounds and career levels plays an important role in strengthening a journal's review system, similar to how harmonious chemistry between junior and senior players enhances a soccer team's performance. We actively encourage junior scientists to engage in the peer review process. However, I would like to use this opportunity to emphasize the importance of involving peers from a diverse spectrum of backgrounds and career stages, including established scholars and industry researchers, in reviewing manuscripts. Since established scholars and industry researchers may offer insights and perspectives that may be overlooked by junior scientists, I hope they also actively join

现在是表彰过去一年中的最佳审稿人的时候了。我们每年都会表彰一位为期刊做出贡献的审稿人,以纪念曼弗雷德-克罗格(Manfred Kroger)。克罗格博士曾长期担任《食品科学与食品安全综合评论》(CRFSFS)的编辑,因其在确保稿件一流方面的勤奋而闻名。为了评选克罗格奖得主,副主编们首先提名在过去几年中为他们做了出色审稿工作的审稿人,并用审稿人的表现数据来支持他们的提名。然后由科学编辑投票决定哪位候选人获奖。该奖项基于多个因素:审稿数量很重要,审稿的及时性和质量也很重要。今年的曼弗雷德-克罗格奖授予阿肯色大学的 Han-Seok Seo 博士,他是感官分析领域的顶尖专家和杰出的审稿人。他在过去 5 年中完成了 90 项评审工作,平均周转天数为 11.6 天。显然,Seo 博士理应获得 2023 年克罗格奖:科学杂志的组织结构与足球队如出一辙。一支典型足球队的位置,即守门员、后卫、中场和前锋,他们的角色分别对应主编、科学/副主编、同行评审员和作者。就像中场球员帮助前锋进球,或在对方球员到达防线前阻挡对方的进球企图一样,同行评审员也为作者提供有益的意见,以提高稿件质量。此外,同行评审员通过认真审稿,在防止不准确、低质量和误导性稿件发表方面发挥着至关重要的作用。正如中场球员被视为足球队的发动机一样,同行评审员也是期刊系统的重要组成部分。当期刊拥有更多训练有素、尽职尽责、热情洋溢的审稿人时,期刊的影响力就会显著提高,就像技术娴熟、尽职尽责、精神饱满的球员能提高足球队的成绩一样。此外,来自不同背景和职业水平的审稿人组合在加强期刊审稿系统方面发挥着重要作用,这就好比初级和高级球员之间的和谐化学反应会提高足球队的表现。我们积极鼓励年轻科学家参与同行评审过程。不过,我想借此机会强调让来自不同背景和职业阶段的同行(包括知名学者和行业研究人员)参与审稿的重要性。由于知名学者和行业研究人员可能会提出一些被初级科学家忽视的见解和观点,因此我希望他们也能积极参与审稿过程。这样,我们就能增强期刊审稿系统和科学界的凝聚力和实力。让我们共同努力,巩固我们的中场地位!谢谢您的意见,徐博士。我从不认为自己是足球队的守门员;相反,我作为主编的工作似乎更像一个放猫人。我们非常感谢他作为《食品科学杂志》中译员所做的工作。真诚的,Rich Hartel 博士《食品科学杂志》主编威斯康星大学麦迪逊分校教授
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引用次数: 0
Using a Centroid-based approach for a reliable identification of morels (Morchella spp.): A case study for food authentication. 使用基于中心点的方法可靠识别羊肚菌(Morchella spp.):食品认证案例研究。
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-02 DOI: 10.1111/1750-3841.17219
Melissa Cravero, Jean Ruelle, Saskia Bindschedler, Stefan Emler, Pilar Junier

Food fraud is a problematic yet common phenomenon in the food industry. It impacts numerous sectors, including the market of edible mushrooms. Morel mushrooms are prized worldwide for their culinary and medicinal use. They represent a taxonomically complex group in which food fraud has already been reported. Among the methods to evaluate food fraud, some rely on comparisons of genetic sequences obtained from a sample to existing databases. However, the quality and usefulness of the results are limited by the type of comparison tool and the quality of the database used. The Centroid-based approach is applied by SmartGene in a proprietary artificial intelligence-based method for the generation of automatically curated reference databases that can be further expert curated. In this study, using sequences of the ribosomal internal transcribed spacer (ITS) of the genus Morchella (true morels), we compared this approach to the traditional pairwise alignment tool using two other databases: UNITE and Mycobank (MLST). The Centroid-based approach using an expert-curated database was more performant for the identification of 53 representative ITS sequences corresponding to validated species (83% accuracy, compared to 36% and 47% accuracy for UNITE and MLST, respectively). The Centroid method also revealed an inaccurate taxonomic annotation for sequences of commercial cultivars submitted to public databases. Combined with the web-based commercial software IDNS® available at Smartgene, the Centroid-based approach constitutes a valuable tool to ensure the quality of morel products on the market for actors of the food industry. PRACTICAL APPLICATION: The Centroid-based approach can be used by agri-food actors who need to identify true morels down to the species level without any prior taxonomical knowledge. These include routine laboratories of the food industry, food distributors, and public surveillance agencies. This is a reliable method that requires minimal skills and resources, therefore being particularly adapted for nonspecialists.

食品欺诈是食品行业的一个常见问题。它影响着众多行业,包括食用菌市场。羊肚菌因其烹饪和药用价值而受到全世界的青睐。莫莱尔蘑菇在分类学上是一个复杂的类群,食品欺诈在该类群中已有报道。在评估食品欺诈的方法中,有些依赖于将从样本中获得的基因序列与现有数据库进行比较。然而,比较结果的质量和实用性受到比较工具类型和所用数据库质量的限制。SmartGene 将基于中心点的方法应用于一种基于人工智能的专有方法中,用于生成可进一步由专家策划的自动策划参考数据库。在这项研究中,我们使用羊肚菌属核糖体内部转录间隔序列(ITS),将这种方法与使用其他两个数据库的传统配对工具进行了比较:UNITE 和 Mycobank (MLST)。基于 Centroid 的方法使用专家整理的数据库,在鉴定与验证物种相对应的 53 个代表性 ITS 序列时表现更佳(准确率为 83%,而 UNITE 和 MLST 的准确率分别为 36% 和 47%)。对于提交到公共数据库的商业栽培品种序列,Centroid 方法也发现分类注释不准确。与 Smartgene 提供的基于网络的商业软件 IDNS® 结合使用,基于 Centroid 的方法成为食品行业确保市场上羊肚菌产品质量的重要工具。实际应用:基于中心点的羊肚菌鉴定方法可用于需要鉴定真正的羊肚菌直至其种类的农业食品从业者,而无需任何分类学知识。这些机构包括食品行业的常规实验室、食品经销商和公共监督机构。这是一种可靠的方法,只需最低限度的技能和资源,因此特别适合非专业人员使用。
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引用次数: 0
Moringa oleifera seed methanol extract with consolidated antimicrobial, antioxidant, anti-inflammatory, and anticancer activities. 油辣木籽甲醇提取物具有综合的抗菌、抗氧化、抗炎和抗癌活性。
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-02 DOI: 10.1111/1750-3841.17223
Esmail M El-Fakharany, Wafaa B Elsharkawy, Yousra A El-Maradny, Hamada El-Gendi

The wide biological activity of the Moringa oleifera represents a potential opportunity for developing selective cancer treatment drugs. The bioactive phytochemicals in Moringa seed extract (MSE) indicated large numbers of phytochemicals (21 compounds) with dominant abundance for cycloisolongifolene, 8,9-dehydro-9-vinyl, and chamazulene accounting for 12.7% and 12.19% of the total detected compounds. The MSE showed a potent anticancer effect toward Caco-2, MDA, and HepG-2 cells with half-maximal inhibitory concentration (IC50) values of 9.15 ± 1.18, 4.85 ± 0.11, and 7.36 ± 0.22 µg/mL, respectively, with higher safety (≥31-folds) toward normal human cells (IC50 of 150.7 ± 11.11 µg/mL). It appears that MSE stimulates selective-dose-dependent cell shrinkage, and nuclear condensation in the tumor cells, which finally induces the apoptosis pathway to increase its anticancer action. Additionally, MSE showed a potent capability to stimulate cell cycle arrest in both main checkpoint phases (G0/G1 and G2/M) of cell population growth. The apoptotic death stimulation was confirmed through upregulation of tumor protein p53 (p53) and cyclin-dependent kinase inhibitor p21 (p21) expression by more than three- to sixfold and downregulation of B-cell lymphoma 2 expression (threefold) in MSE-treated cells compared to 5-fluorouracil (5-FU)-treated tumor cells. Furthermore, the MSE revealed strong anti-inflammatory activity with significant antioxidant activity by lowering nitric oxide levels and enhancing the superoxide dismutase activity. On the other hand, the MSE revealed broad-spectrum antibacterial activity in a dose-dependent manner against Staphylococcus aureus minimum inhibitory concentration (MIC of 1.25 mg/mL), followed by Salmonella typhimurium (MIC of 1.23 mg/mL), whereas Escherichia coli was the least sensitive to MSE activity (MIC of 22.5 mg/mL) with significant antibiofilm activity against sensitive pathogens.

Moringa oleifera 的广泛生物活性为开发选择性癌症治疗药物提供了潜在机会。辣木籽提取物(MSE)中的生物活性植物化学物质显示出大量的植物化学物质(21 种化合物),其中环异龙脑烯、8,9-脱氢-9-乙烯基和香茅烯的含量占检测到的化合物总量的 12.7% 和 12.19%。MSE 对 Caco-2、MDA 和 HepG-2 细胞具有强效抗癌作用,其半最大抑制浓度(IC50)值分别为 9.15 ± 1.18、4.85 ± 0.11 和 7.36 ± 0.22 µg/mL,对正常人细胞的安全性更高(≥31 倍)(IC50 为 150.7 ± 11.11 µg/mL)。由此看来,MSE 可刺激肿瘤细胞发生选择性剂量依赖性细胞萎缩和核凝缩,最终诱导细胞凋亡途径,从而增强其抗癌作用。此外,MSE 还能在细胞群生长的两个主要检查点阶段(G0/G1 和 G2/M)刺激细胞周期停滞。与5-氟尿嘧啶(5-FU)处理的肿瘤细胞相比,MSE处理的细胞中肿瘤蛋白p53(p53)和细胞周期蛋白依赖性激酶抑制剂p21(p21)的表达上调了3至6倍,B细胞淋巴瘤2的表达下调了3倍,从而证实了MSE对细胞凋亡的刺激作用。此外,MSE 还通过降低一氧化氮水平和提高超氧化物歧化酶活性,显示出强大的抗炎活性和显著的抗氧化活性。另一方面,MSE 还具有广谱抗菌活性,对金黄色葡萄球菌的最小抑菌浓度(MIC 为 1.25 毫克/毫升)呈剂量依赖性,其次是伤寒沙门氏菌(MIC 为 1.23 毫克/毫升),而大肠杆菌对 MSE 活性最不敏感(MIC 为 22.5 毫克/毫升),对敏感病原体具有显著的抗生物膜活性。
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引用次数: 0
Kinetics of chemical and color changes in wheat and water during atmospheric cooking as affected by the acidity, hardness, and iron content of water. 大气蒸煮过程中小麦和水的化学和颜色变化动力学受水的酸度、硬度和铁含量的影响。
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-02 DOI: 10.1111/1750-3841.17222
Sadiye Michel, Mustafa Bayram

Color changes in wheat and cooking water, which affect the quality of bulgur and wastewater, are important. Understanding the impacts of cooking water acidity, hardness, and iron content is significant for producing bright-yellow colored bulgur and determining the possible negative effects of cooking water on the environment. Thereby, the gelatinization degree and color (L*, a*, b*, and yellowness index) of wheat cooked with waters at different pH (3, 5, 7, 9, and 11), hardness (soft, hard, and very hard), and iron content (0, 1, and 2 mg/L) were determined every 10 min of cooking. pH, Brix, conductivity, hardness, turbidity, and color of cooking waters were also determined and kinetically modeled. After cooking, it was revealed that cooking with water at pH 3 favored the color of cooked wheat, whereas pH 11 caused darkening. Nevertheless, as the wastewater pH of cooking waters with pH 3 and 11 may be harmful to the environment, it is recommended to use water in the range of pH 5-9 for bulgur production. Cooking with very hard water is also not recommended as it causes some adverse effects such as diminishing the gelatinization rate in wheat, increasing the cooking time, and negatively affecting the color.

小麦和蒸煮水的颜色变化会影响到牛杂粮和废水的质量,因此非常重要。了解蒸煮水酸度、硬度和铁含量的影响,对于生产颜色鲜黄的粗粮和确定蒸煮水可能对环境造成的负面影响意义重大。因此,使用不同 pH 值(3、5、7、9 和 11)、硬度(软、硬和极硬)和铁含量(0、1 和 2 mg/L)的水烹煮小麦,每 10 分钟测定一次其糊化度和颜色(L*、a*、b* 和黄度指数)。蒸煮后发现,用 pH 值为 3 的水进行蒸煮有利于煮熟小麦的颜色,而 pH 值为 11 的水则会导致颜色变深。不过,由于 pH 值为 3 和 11 的烹饪用水的废水 pH 值可能会对环境造成危害,因此建议使用 pH 值在 5-9 之间的水生产粗粮。此外,也不建议使用硬度很高的水进行烹饪,因为它会造成一些不利影响,如降低小麦的糊化率、增加烹饪时间和对颜色产生负面影响。
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引用次数: 0
Effect of glycosylation modification on structure and properties of soy protein isolate: A review. 糖基化修饰对大豆分离蛋白结构和特性的影响:综述。
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-02 DOI: 10.1111/1750-3841.17181
Jinjing Chen, Wanting Zhang, Yiming Chen, Meng Li, Chang Liu, Xiuli Wu

Soybean protein isolate (SPI) is a highly functional protein source used in various food applications, such as emulsion, gelatin, and food packaging. However, its commercial application may be limited due to its poor mechanical properties, barrier properties, and high water sensitivity. Studies have shown that modifying SPI through glycosylation can enhance its functional properties and biological activities, resulting in better application performance. This paper reviews the recent studies on glycosylation modification of SPI, including its quantification method, structural improvements, and enhancement of its functional properties, such as solubility, gelation, emulsifying, and foaming. The review also discusses how glycosylation affects the bioactivity of SPI, such as its antioxidant and antibacterial activity. This review aims to provide a reference for further research on glycosylation modification and lay a foundation for applying SPI in various fields.

大豆分离蛋白(SPI)是一种高功能蛋白质来源,可用于乳液、明胶和食品包装等各种食品应用中。然而,由于其机械性能、阻隔性能和高水敏性较差,其商业应用可能会受到限制。研究表明,通过糖基化修饰 SPI 可以提高其功能特性和生物活性,从而获得更好的应用性能。本文综述了最近对 SPI 进行糖基化改性的研究,包括其量化方法、结构改进和功能特性的增强,如溶解性、凝胶化、乳化和发泡。综述还讨论了糖基化如何影响 SPI 的生物活性,如抗氧化和抗菌活性。本综述旨在为糖基化修饰的进一步研究提供参考,并为 SPI 在各个领域的应用奠定基础。
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引用次数: 0
Fat, protein, and temperature each contribute to reductions in capsaicin oral burn. 脂肪、蛋白质和温度都有助于减少辣椒素的口腔燃烧。
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-02 DOI: 10.1111/1750-3841.17221
Justin Gaiser, John E Hayes

It is widely accepted that milk provides the greatest relief from capsaicin burn, an effect typically attributed to its fat content and temperature. Previously, Lawless et al. reported partitioning lipophilic capsaicin in fat reduces burn, whereas Green showed lower temperature reduces burn. Recent research shows that dairy and nondairy proteins also reduce capsaicin burn, suggesting that multiple factors reduce oral burn from chilies. Here, we investigated the effectiveness of palate cleansers with varied viscosities, temperatures, and sugar, fat, and protein content. Specifically, we tested ice cream, Italian ice, yogurt, lassi, cold water (4°C), and warm water (37°C). Participants rinsed with a 5 ppm capsaicin solution, followed by a palate cleanser, before rating burn intensity continuously for 2 min on a general Labeled Magnitude Scale. Inspection of the time-intensity (TI) curves revealed all palate cleansers performed better than warm water. Italian ice performed on par with cold water, which did better than yogurt. Pairwise comparisons showed that ice cream and lassi had significantly lower burn ratings at some time points relative to warm or cold water. We extracted scaffolding parameters for each TI curve, finding that ice cream and lassi had the lowest areas-under-the-curve and the greatest percent decrease from their maxima, with ice cream performing slightly better in both parameters. These data support the view that it is not just one characteristic of a product, but rather a combination of product factors that reduce oral burn, including fat content, protein content, and temperature. More research is required to determine the relative weight of these factors in combination, given the multiple mechanisms underlying burn reduction.

人们普遍认为,牛奶能最大程度地缓解辣椒素灼伤,这种效果通常归因于牛奶中的脂肪含量和温度。此前,Lawless 等人报告称,将亲脂性辣椒素分装在脂肪中可减轻灼烧感,而 Green 则显示较低的温度可减轻灼烧感。最近的研究表明,乳制品和非乳制品蛋白质也会降低辣椒素的灼烧感,这表明多种因素会降低辣椒的口腔灼烧感。在这里,我们研究了不同粘度、温度以及糖、脂肪和蛋白质含量的口腔清洁剂的效果。具体来说,我们测试了冰淇淋、意大利冰、酸奶、拉丝、冷水(4°C)和温水(37°C)。参与者先用百万分之 5 的辣椒素溶液冲洗,再用口腔清洁剂清洗,然后在一般标签量表上连续 2 分钟评定灼烧强度。时间-强度(TI)曲线显示,所有洁厕液的表现都优于温水。意大利冰的表现与冷水相当,而冷水的表现优于酸奶。配对比较显示,冰淇淋和拉丝在某些时间点的灼烧评分明显低于温水或冷水。我们提取了每条 TI 曲线的支架参数,发现冰淇淋和拉丝的曲线下面积最小,从最大值开始的下降百分比最大,而冰淇淋在这两个参数上都略胜一筹。这些数据支持了这样一种观点,即降低口腔燃烧的不仅仅是产品的一种特性,而是产品因素的组合,包括脂肪含量、蛋白质含量和温度。考虑到减少燃烧的多重机制,还需要更多的研究来确定这些因素在组合中的相对权重。
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引用次数: 0
Untargeted metabolomics combined with vitro antioxidant to comprehensively evaluate the effect of sodium sulfite immersion on the holistic quality of mung bean sprouts. 非靶向代谢组学结合体外抗氧化全面评估亚硫酸钠浸泡对绿豆芽整体质量的影响
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-26 DOI: 10.1111/1750-3841.17147
Yaxin Li, Zheng Zhou, Qi Wu, Baoguo Chen, Shuhong Ye, Yuna Cui, Yan Ding

Mung bean sprouts are widely consumed as a seasonal fresh vegetable, renowned for their affordability and richness in antioxidants and bioactive compounds. This study employed ultra-high-performance liquid chromatogram-Q-Exactive HF mass spectrometry (UHPLC-QE-MS) and multivariate statistical analysis to comprehensively evaluate the chemical profile of mung bean sprouts following sulfite immersion. The findings revealed a significant alteration in the overall chemical composition of mung bean sprouts following sodium sulfite immersion. Eleven components, including four sulfur-containing compounds, were identified as characteristic markers distinguishing between non-immersed and sodium sulfite-immersed mung bean sprouts. Esterification and addition reactions were inferred to occur during sodium sulfite immersion, leading to the transformation of flavonoid and saponin sulfates. Commercial samples analysis indicated that sulfur-containing compounds were detectable in 9 of 11 commercial mung bean sprouts. Meanwhile, when sodium sulfite concentration exceeded 3.00 mg/mL and immersion time exceeded 360 min, the contents of total polyphenol and flavonoid were significantly reduced and the antioxidant activity was adversely influenced.

绿豆芽作为一种时令新鲜蔬菜被广泛食用,因其价格低廉、富含抗氧化剂和生物活性化合物而闻名。本研究采用超高效液相色谱-Q-Exactive HF 质谱(UHPLC-QE-MS)和多元统计分析方法,对亚硫酸盐浸泡绿豆芽后的化学成分进行了全面评估。研究结果表明,亚硫酸钠浸泡绿豆芽后,绿豆芽的整体化学成分发生了重大变化。经鉴定,包括四种含硫化合物在内的 11 种成分是区分未浸泡绿豆芽和亚硫酸钠浸泡绿豆芽的特征标记。推断亚硫酸钠浸泡过程中会发生酯化和加成反应,导致类黄酮和皂素硫酸盐的转化。商业样品分析表明,在 11 个商业绿豆芽中,有 9 个能检测到含硫化合物。同时,当亚硫酸钠浓度超过 3.00 毫克/毫升、浸泡时间超过 360 分钟时,总多酚和类黄酮的含量会显著降低,抗氧化活性也会受到不利影响。
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引用次数: 0
Inoculation of Zaopei with biofortification: Inducing variation in community structure and improving flavor compounds in Nongxiangxing baijiu. 用生物强化法接种枣皮:诱导群落结构变异,改善农香行白酒的风味化合物。
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-26 DOI: 10.1111/1750-3841.17187
Yijiao Wu, Pei Xu, Xianping Qiu, Yichen Mao, Jianshen Yu, Lei Tian, Yanzhong Li, Juan Zhang, Xiangting Zhu, Ying Liu, Hongguang Shang, Tongwei Guan

The deterioration of the quality of raw liquor caused by the low content of ethyl hexanoate in Nongxiangxing baijiu has become a pervasive problem in the baijiu industry. Therefore, this study attempted to increase the synthesis of ethyl hexanoate by microorganisms with high esterase activity to increase Zaopei fermentation. The results showed that biofortification was a feasible and important way to improve the quality of the raw liquor and increase the ethyl hexanoate content. Adding Bacillus subtilis, Staphylococcus epidermidis, and Millerozyma farinosa for biofortified fermentation disturbed the microbial community structure of Zaopei and increased the abundance of Wickerhamomyces, Saccharomyces, and Thermoascus. The contents of ethyl hexanoate, ethyl valerate, ethyl caprylate, and ethyl heptanoate also increased noticeably in baijiu. The results of E-nose and sensory analysis tested and verified that the baijiu in the fortified group had better flavor characteristics.

农乡行白酒中己酸乙酯含量低导致原酒质量下降已成为白酒行业普遍存在的问题。因此,本研究尝试用酯酶活性高的微生物来增加己酸乙酯的合成,以提高枣皮发酵度。结果表明,生物强化是改善原酒质量、提高己酸乙酯含量的可行且重要的方法。在生物强化发酵过程中添加枯草芽孢杆菌、表皮葡萄球菌和法氏米勒氏菌扰乱了枣泥的微生物群落结构,增加了酵母菌、酵母菌和嗜热菌的丰度。白酒中己酸乙酯、戊酸乙酯、辛酸乙酯和庚酸乙酯的含量也明显增加。电子鼻和感官分析的结果表明,强化组的白酒具有更好的风味特征。
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引用次数: 0
Effect of linolenic acid on triterpenoids production by the liquid fermentation of Antrodia cinnamomea. 亚麻酸对肉桂蚁液体发酵产生三萜类化合物的影响
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-26 DOI: 10.1111/1750-3841.17170
Wei Tang, Longfei Ye, Meizhu Guan, Jianfei He, Jianhua Liu, Peicheng Zhao

Liquid state fermentation is now a commonly used route to obtain triterpenoids from Antrodia cinnamomea, and linolenic acid can significantly promote triterpenoids synthesis, whereas its action mechanism has not been studied. Here, we comprehensively performed an investigation on the mechanism of linolenic acid to promote triterpenoids production in liquid-state fermentation of A. cinnamomea. Results showed that the addition of linolenic acid increased the unsaturated fatty acid index, fluidity, and permeability in the cell membrane of A. cinnamomea mycelia, favored the absorption of nutrients in the medium by the mycelium, enhanced the material exchange inside and outside, and thus promoted mycelial growth and triterpenoids synthesis. Moreover, 767 significantly differentially expressed genes were detected by adding linolenic acid, including 212 upregulated genes and 555 downregulated genes. The upregulated genes were mainly enriched in metabolism, glycolytic pathway, TCA cycle, and pyruvate metabolism. It was seen that the addition of linolenic acid improved the cell metabolic activity and promoted the synthesis of secondary metabolites, proving that the addition of linolenic acid improved the metabolic viability of cells and promoted secondary metabolite synthesis.

液态发酵是目前从肉桂蚁中获取三萜类化合物的常用途径,亚麻酸能显著促进三萜类化合物的合成,但其作用机制尚未研究清楚。在此,我们对亚麻酸促进肉桂酸液态发酵生产三萜类化合物的机理进行了全面研究。结果表明,亚麻酸的添加提高了肉桂酸菌丝体细胞膜的不饱和脂肪酸指数、流动性和通透性,有利于菌丝体吸收培养基中的营养物质,增强了菌丝体内外的物质交换,从而促进了菌丝体的生长和三萜类化合物的合成。此外,添加亚麻酸还检测到 767 个明显差异表达的基因,包括 212 个上调基因和 555 个下调基因。上调基因主要集中在新陈代谢、糖酵解途径、TCA 循环和丙酮酸代谢中。可见,亚麻酸的添加提高了细胞的代谢活性,促进了次生代谢物的合成,证明亚麻酸的添加提高了细胞的代谢活力,促进了次生代谢物的合成。
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引用次数: 0
Infusion of active compound into sliced button mushrooms through vacuum impregnation to improve functionality: Comparing response surface methodology and artificial neural network. 通过真空浸渍将活性化合物注入切片金针菇以提高其功能:响应面方法与人工神经网络的比较。
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-26 DOI: 10.1111/1750-3841.17166
Muktabai Dinesh Wagh, Mohammed Shafiq Alam, Tapas Roy, Satish Kumar Gupta

The present study explores the infusion of active compounds (ascorbic acid and calcium lactate) into sliced button mushrooms (Agaricus bisporus) to increase the nutritional value and reduce the browning effect of sliced mushrooms using the vacuum impregnation (VI) technique. The aim was to functionalize the vacuum-infused sliced mushrooms and evaluate the physicochemical properties of button mushrooms for diversifying food use. The central composite design was implemented to determine the optimized condition for the process with four independent factors, that is, immersion time (IT) 30-90 min, solution temperature (ST) 35-55°C, solution concentration (SC) 4%-12%, and vacuum pressure (VP) 50-170 mbar. The optimum VI processes obtained were ST-40°C, SC-8%, VP-140 mbar, and IT-65 min with a desirability function of 0.77. Statistically, two models (response surface methodology [RSM] and artificial neural network [ANN]) were employed to compare the better performance for the prediction of VI operational process parameters. The RSM model showed a better prediction of VI process parameters than the ANN model, with a higher R2 value (0.9228 vs. 0.8160) and lower root mean square error value (1.4004 vs. 2.1751), χ2 (2.4491 vs. 5.2762), mean absolute error (1.1177 vs. 1.1611), and absolute average deviation (4.3532 vs. 5.6746) for water loss. A similar pattern was observed for solute gain, ascorbic acid, titratable acidity, color change, firmness, and pH. Therefore, the VI process was found to be an effective method for enhancing the nutritional properties of sliced mushrooms. These findings concluded that the RSM model is more efficient for better prediction with good accuracy of the VI process than the ANN model.

本研究采用真空浸渍(VI)技术,探索将活性化合物(抗坏血酸和乳酸钙)注入金针菇(双孢蘑菇)片中,以提高营养价值并降低蘑菇片的褐变效果。目的是对真空浸渍的蘑菇片进行功能化处理,并评估金针菇的理化特性,以实现食品用途的多样化。该研究采用中心复合设计法来确定工艺的优化条件,其中包含四个独立因素,即浸泡时间(IT)30-90 分钟、溶液温度(ST)35-55°C、溶液浓度(SC)4%-12% 和真空压力(VP)50-170 毫巴。最佳 VI 过程为 ST-40°C、SC-8%、VP-140 毫巴和 IT-65 分钟,理想函数为 0.77。在统计学上,采用了两种模型(响应面方法和人工神经网络)来比较预测 VI 操作工艺参数的较佳性能。RSM 模型对 VI 工艺参数的预测效果优于 ANN 模型,其 R2 值(0.9228 对 0.8160)更高,均方根误差值(1.4004 对 2.1751)、χ2(2.4491 对 5.2762)、平均绝对误差(1.1177 对 1.1611)和绝对平均偏差(4.3532 对 5.6746)更低。在溶质增加、抗坏血酸、可滴定酸度、颜色变化、紧实度和 pH 值方面也观察到类似的模式。因此,VI 工艺被认为是提高切片蘑菇营养特性的有效方法。这些研究结果得出结论,RSM 模型比 ANN 模型更有效,能更准确地预测 VI 过程。
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引用次数: 0
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Journal of Food Science
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