Molecular mechanism of lncRNAs in ovarian cancer: lncRNA CASC19 regulates the malignant progression of ovarian cancer through miR-761/CBX2 axis

Abstract

Background: Long non-coding RNAs (lncRNAs) are involved in the numerous types of tumors. The aim of this study is to comprehend the pathological mechanism of lncRNA CASC19 in ovarian cancer. Methods: CASC19, miR-761 and CBX2 expression in the samples was quantitatively detected by real-time quantitative polymerase chain reaction (RT-qPCR) reaction. The proliferation and apoptosis levels of ovarian cancer cells were evaluated via cell counting kit-8 (CCK-8) and Flow cytometry assays. The targeting relationship and correlation of CASC19, miR-761 and CBX2 were investigated through luciferase reporter assay and Pearson assay. Results: CASC19 and CBX2 were enriched in ovarian cancer, while miR-761 expression was decreased. Silencing CASC19 suppressed the cell growth and promoted cell apoptosis. CASC19 targeted the inhibition of miR-761 expression and regulated the level of CBX2. In addition, the miR-761 inhibitor reversed the inhibitory effect of si-CASC19 on the biological activity of ovarian cancer cells, while si-CBX2 restored the regulation of miR-761 inhibitor on the development of ovarian cancer. Conclusion: LncRNA CASC19 mediated the malignant progression of ovarian cancer through miR-761/CBX2 axis, which provided a potential therapeutic target for the cure of patients.