Pub Date : 2024-09-01DOI: 10.1615/jenvironpatholtoxicoloncol.2024053617
Huaigao Wang, Jizhen Liu, Mengjie Shi, Yuanye Xue, Xinsheng Peng, Bin Gan, Yanfang Zhou, Zhidong Li
The Hippo pathway and its transcription co-activator YAP play a critical role in the regulation of cell proliferation, apoptosis and the control of organ size. In the past several years, YAP has been found to be expressed in various human cancers, however, its expression in Nasopharyngeal Carcinoma ( NPC ) remains unstudied. In this report, we found that YAP was overexpressed in human NPC tissues, and its expression was also significantly higher in five NPC cell lines when compared to the nasopharyngeal epithelial cell line NP69 (p<0.01). CNE1 and CNE1-GL cells treated with EOS had an enhanced expression level of phosphorylated YAP and downregulated expression of survivin, which inhibits the proliferation of CNE1 and CNE1-GL cells. These results highlight an important role of hippo pathway in the growth of nasopharyngeal carcinoma and implicate a potential mechanism of ethyl acetate extract of oratosquilla（EOS） preventing the proliferation of nasopharyngeal carcinoma.
{"title":"Ethyl Acetate Extract of Oratosquilla Inhibits the Growth of Nasopharyngeal Carcinoma through the Hippo Pathway","authors":"Huaigao Wang, Jizhen Liu, Mengjie Shi, Yuanye Xue, Xinsheng Peng, Bin Gan, Yanfang Zhou, Zhidong Li","doi":"10.1615/jenvironpatholtoxicoloncol.2024053617","DOIUrl":"https://doi.org/10.1615/jenvironpatholtoxicoloncol.2024053617","url":null,"abstract":"The Hippo pathway and its transcription co-activator YAP play a critical role in the regulation of cell proliferation, apoptosis and the control of organ size. In the past several years, YAP has been found to be expressed in various human cancers, however, its expression in Nasopharyngeal Carcinoma ( NPC ) remains unstudied. In this report, we found that YAP was overexpressed in human NPC tissues, and its expression was also significantly higher in five NPC cell lines when compared to the nasopharyngeal epithelial cell line NP69 (p<0.01). CNE1 and CNE1-GL cells treated with EOS had an enhanced expression level of phosphorylated YAP and downregulated expression of survivin, which inhibits the proliferation of CNE1 and CNE1-GL cells. These results highlight an important role of hippo pathway in the growth of nasopharyngeal carcinoma and implicate a potential mechanism of ethyl acetate extract of oratosquilla（EOS） preventing the proliferation of nasopharyngeal carcinoma.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"774 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142210273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-01DOI: 10.1615/jenvironpatholtoxicoloncol.2024053370
Kai Zhu, Zhenwu Xu, Xue Peng, Chenhua Xu, Shengjia Chen
Background: Growth hormone-releasing hormone (GH-RH) and its antagonists are believed to influence the progression of varying tumor diseases. However, the specific effects of GH-RH-related genes on lung adenocarcinoma (LUAD) are yet to be deciphered. Methods: GH-RH-related gene set data, LUAD transcriptome data, and clinical data were available for download at GeneCards, TCGA, and GEO databases. R software was implemented to finish differential, regression, cluster, survival, and tumor microenvironment analyses. Drugs associated with model genes were predicted using CellMiner database. Results: 781 LUAD samples (TCGA-LUAD: 600 cases; GSE50081: 181 cases) and data for 1555 GH-RH-related gene sets were obtained from public datasets. Two LUAD subtypes with different GH-RH gene expressions were identified through cluster analysis. Significant differences were unveiled in prognosis between the two subtypes. A prognostic risk scoring model was generated based on genes screened from the PPI network, and afterward, the model was validated. The model comprised 7 genes, specifically CLCA1, CYP17A1, DKK1, IGF2BP1, IGFBP1, RPE65, and VGF. Immune-related analysis revealed significant differences in immune cell infiltration levels between high and low-risk groups (P<0.05), with mast cells and neutrophils showing significantly higher infiltration levels in the low-risk population than the other group. Conclusion: The 7-gene signature in the current study is of paramount importance for predicting overall survival and immune microenvironment of LUAD patients.
{"title":"Identification of Lung Adenocarcinoma Subtypes by Using Growth Hormone-Releasing Hormone-Related Genes and Establishment of Signature to Predict Prognosis and Guide Immunother","authors":"Kai Zhu, Zhenwu Xu, Xue Peng, Chenhua Xu, Shengjia Chen","doi":"10.1615/jenvironpatholtoxicoloncol.2024053370","DOIUrl":"https://doi.org/10.1615/jenvironpatholtoxicoloncol.2024053370","url":null,"abstract":"Background: Growth hormone-releasing hormone (GH-RH) and its antagonists are believed to influence the progression of varying tumor diseases. However, the specific effects of GH-RH-related genes on lung adenocarcinoma (LUAD) are yet to be deciphered.\u0000Methods: GH-RH-related gene set data, LUAD transcriptome data, and clinical data were available for download at GeneCards, TCGA, and GEO databases. R software was implemented to finish differential, regression, cluster, survival, and tumor microenvironment analyses. Drugs associated with model genes were predicted using CellMiner database.\u0000Results: 781 LUAD samples (TCGA-LUAD: 600 cases; GSE50081: 181 cases) and data for 1555 GH-RH-related gene sets were obtained from public datasets. Two LUAD subtypes with different GH-RH gene expressions were identified through cluster analysis. Significant differences were unveiled in prognosis between the two subtypes. A prognostic risk scoring model was generated based on genes screened from the PPI network, and afterward, the model was validated. The model comprised 7 genes, specifically CLCA1, CYP17A1, DKK1, IGF2BP1, IGFBP1, RPE65, and VGF. Immune-related analysis revealed significant differences in immune cell infiltration levels between high and low-risk groups (P<0.05), with mast cells and neutrophils showing significantly higher infiltration levels in the low-risk population than the other group.\u0000Conclusion: The 7-gene signature in the current study is of paramount importance for predicting overall survival and immune microenvironment of LUAD patients.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"44 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142210272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Gastric cancer (GC) is a widespread cancerous disease with an unfavorable prognosis, linc01105 appears to have a significant connection with the development of GC, but the precise regulatory mechanism remains obscure. Methods: RT-qPCR was utilized to determine the expression of linc01105, miR-650, and TCEA3. Proliferative capacity of GC cells was measured by CCK8. Cell apoptosis rates were analyzed via flow cytometry assessment. The invasive capability was assessed through the transwell experiment. The targeted regulation of linc01105, miR-650, and TCEA3 was validated through dual luciferase reporter gene assay. Result: Low expression of linc01105, high expression of miR-650, and low expression of TCEA3 were observed in GC tumor tissues and cell lines. High expression of linc01105 was correlated with a positive prognosis in GC patients. Linc01105 affected GC cell function by miR-650/TCEA3 axis. Conclusion: This study suggested that linc01105 may possess inhibitory functions towards GC. In addition, linc01105 may be a prognostic marker for GC.
{"title":"LncRNA linc01105 inhibits gastric cancer growth and metastasis by regulating the miR-650/TCEA3 axis","authors":"Qiang Zhang, Hui Mi, Huimei Cai, Chenhui Li, Zhou Wu, Jianfang Zhang","doi":"10.1615/jenvironpatholtoxicoloncol.2024054112","DOIUrl":"https://doi.org/10.1615/jenvironpatholtoxicoloncol.2024054112","url":null,"abstract":"Background: Gastric cancer (GC) is a widespread cancerous disease with an unfavorable prognosis, linc01105 appears to have a significant connection with the development of GC, but the precise regulatory mechanism remains obscure.\u0000Methods: RT-qPCR was utilized to determine the expression of linc01105, miR-650, and TCEA3. Proliferative capacity of GC cells was measured by CCK8. Cell apoptosis rates were analyzed via flow cytometry assessment. The invasive capability was assessed through the transwell experiment. The targeted regulation of linc01105, miR-650, and TCEA3 was validated through dual luciferase reporter gene assay.\u0000Result: Low expression of linc01105, high expression of miR-650, and low expression of TCEA3 were observed in GC tumor tissues and cell lines. High expression of linc01105 was correlated with a positive prognosis in GC patients. Linc01105 affected GC cell function by miR-650/TCEA3 axis.\u0000Conclusion: This study suggested that linc01105 may possess inhibitory functions towards GC. In addition, linc01105 may be a prognostic marker for GC.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"15 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141883608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-01DOI: 10.1615/jenvironpatholtoxicoloncol.2024053797
KharChuen Wong, Peng Dai, Yuan Li, Li Liu, Shuanghua Liang
Background: Long non-coding RNAs (lncRNAs) are involved in the numerous types of tumors. The aim of this study is to comprehend the pathological mechanism of lncRNA CASC19 in ovarian cancer. Methods: CASC19, miR-761 and CBX2 expression in the samples was quantitatively detected by real-time quantitative polymerase chain reaction (RT-qPCR) reaction. The proliferation and apoptosis levels of ovarian cancer cells were evaluated via cell counting kit-8 (CCK-8) and Flow cytometry assays. The targeting relationship and correlation of CASC19, miR-761 and CBX2 were investigated through luciferase reporter assay and Pearson assay. Results: CASC19 and CBX2 were enriched in ovarian cancer, while miR-761 expression was decreased. Silencing CASC19 suppressed the cell growth and promoted cell apoptosis. CASC19 targeted the inhibition of miR-761 expression and regulated the level of CBX2. In addition, the miR-761 inhibitor reversed the inhibitory effect of si-CASC19 on the biological activity of ovarian cancer cells, while si-CBX2 restored the regulation of miR-761 inhibitor on the development of ovarian cancer. Conclusion: LncRNA CASC19 mediated the malignant progression of ovarian cancer through miR-761/CBX2 axis, which provided a potential therapeutic target for the cure of patients.
{"title":"Molecular mechanism of lncRNAs in ovarian cancer: lncRNA CASC19 regulates the malignant progression of ovarian cancer through miR-761/CBX2 axis","authors":"KharChuen Wong, Peng Dai, Yuan Li, Li Liu, Shuanghua Liang","doi":"10.1615/jenvironpatholtoxicoloncol.2024053797","DOIUrl":"https://doi.org/10.1615/jenvironpatholtoxicoloncol.2024053797","url":null,"abstract":"Background: Long non-coding RNAs (lncRNAs) are involved in the numerous types of tumors. The aim of this study is to comprehend the pathological mechanism of lncRNA CASC19 in ovarian cancer.\u0000Methods: CASC19, miR-761 and CBX2 expression in the samples was quantitatively detected by real-time quantitative polymerase chain reaction (RT-qPCR) reaction. The proliferation and apoptosis levels of ovarian cancer cells were evaluated via cell counting kit-8 (CCK-8) and Flow cytometry assays. The targeting relationship and correlation of CASC19, miR-761 and CBX2 were investigated through luciferase reporter assay and Pearson assay.\u0000Results: CASC19 and CBX2 were enriched in ovarian cancer, while miR-761 expression was decreased. Silencing CASC19 suppressed the cell growth and promoted cell apoptosis. CASC19 targeted the inhibition of miR-761 expression and regulated the level of CBX2. In addition, the miR-761 inhibitor reversed the inhibitory effect of si-CASC19 on the biological activity of ovarian cancer cells, while si-CBX2 restored the regulation of miR-761 inhibitor on the development of ovarian cancer.\u0000Conclusion: LncRNA CASC19 mediated the malignant progression of ovarian cancer through miR-761/CBX2 axis, which provided a potential therapeutic target for the cure of patients.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"34 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141883496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.1615/jenvironpatholtoxicoloncol.2024053337
Chong Cheng, Ling Zhang, Quanyong Wang, Mingyou Yang, Wenlin Liu
Background: The prevalent histological variant within differentiated thyroid carcinoma is papillary thyroid carcinoma, also known as PTC. The study investigated the clinical performance of serum hsa_circ¬_0001955 in predicting the prognosis of PTC treated with radical thyroidectomy and iodine 131 nail clearance. Method: The relative expression of serum circ_0001955 of PTC patients was detected before and after accepting radical thyroidectomy combined with 131I thyroid remnant ablation by RT-qPCR. Serum thyroglobulin (Tg) and thyroglobulin antibody (TgAb) levels were quantified by an automatic chemiluminescence immunoassay analyzer. Multivariate logistic regression analysis was employed to investigate the risk factors associated with the prognosis of PTC patients with postoperative 131I therapy. Results: The serum circ_0001955 levels in 127 PTC patients were higher than that in 96 multinodular goiter patients and 110 healthy controls before treatment and had diagnostic values for PTC patients. After 131I treatment, serum circ_0001955 levels and Tg value have a correlation with potential recurrence (WBS positive). Serum circ_0001955, Tg, and TgAb value, and their combination may have diagnostic value in predicting recurrence. Conclusion: Serum circ_0001955 levels in patients with PTC after radical thyroidectomy and iodine 131 thyroidectomy may help predict recurrence.
{"title":"Analysis of the clinical value of hsa_circ_0001955 in papillary thyroid cancer treated with 131 iodine","authors":"Chong Cheng, Ling Zhang, Quanyong Wang, Mingyou Yang, Wenlin Liu","doi":"10.1615/jenvironpatholtoxicoloncol.2024053337","DOIUrl":"https://doi.org/10.1615/jenvironpatholtoxicoloncol.2024053337","url":null,"abstract":"Background: The prevalent histological variant within differentiated thyroid carcinoma is papillary thyroid carcinoma, also known as PTC. The study investigated the clinical performance of serum hsa_circ¬_0001955 in predicting the prognosis of PTC treated with radical thyroidectomy and iodine 131 nail clearance.\u0000Method: The relative expression of serum circ_0001955 of PTC patients was detected before and after accepting radical thyroidectomy combined with 131I thyroid remnant ablation by RT-qPCR. Serum thyroglobulin (Tg) and thyroglobulin antibody (TgAb) levels were quantified by an automatic chemiluminescence immunoassay analyzer. Multivariate logistic regression analysis was employed to investigate the risk factors associated with the prognosis of PTC patients with postoperative 131I therapy.\u0000Results: The serum circ_0001955 levels in 127 PTC patients were higher than that in 96 multinodular goiter patients and 110 healthy controls before treatment and had diagnostic values for PTC patients. After 131I treatment, serum circ_0001955 levels and Tg value have a correlation with potential recurrence (WBS positive). Serum circ_0001955, Tg, and TgAb value, and their combination may have diagnostic value in predicting recurrence.\u0000Conclusion: Serum circ_0001955 levels in patients with PTC after radical thyroidectomy and iodine 131 thyroidectomy may help predict recurrence.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"25 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141743969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.1615/jenvironpatholtoxicoloncol.2024053641
Fen Fu, Liju Nie, Linfeng Huang, Qizhou Zhu, Qinglan Yao, Yiguo Wu, Lu Zhao, Lamei Yu
Background: The hypoxic microenvironment of tumor cells is closely related to the progression of ovarian cancer. This study aims to explore the role of matrix-remodeling associated 5 (MXRA5) in regulating cellular hypoxia in ovarian cancer. Methods: The MXRA5 expression and its clinical significance in ovarian cancer were evaluated using GEPIA2, Kaplan-Meier plotter databases, and immunohistochemistry assay. Ovarian cancer cells were treated with normoxia and hypoxia conditions. The siRNAs were used to knock down the MXRA5 expression in hypoxic cells. The cellular capacities were detected by CCK-8 assay, EdU assay, Transwell invasion assay, and TUNEL assay, respectively. Results: The MXRA5 level was increased in ovarian cancer and associated with the PFS and survival of ovarian cancer patients. The proliferation and invasion abilities of ovarian cancer cells were promoted, while apoptosis capacities were inhibited in hypoxic cells. After the knockdown of MXRA5 in hypoxic cells, the proliferation and invasion abilities of the cells were reduced, and the apoptosis capacities were enhanced. Moreover, mechanistically, HIF-1α is a key transcription factor in response to hypoxia that binds to the MXRA5 promoter. Conclusion: MXRA5 expression was induced by hypoxia and had prognostic performance in ovarian cancer. MXRA5 is one of the potential targets of tumor hypoxia regulation. Knockdown of MXRA5 can inhibit proliferation and invasion in ovarian cancer cells caused by HIF-1α, revealing that MXRA5 is one potential targets for tumor hypoxia regulation in ovarian cancer.
{"title":"HIF-1α activates hypoxia-induced MXRA5 expression in the progression of ovarian cancer","authors":"Fen Fu, Liju Nie, Linfeng Huang, Qizhou Zhu, Qinglan Yao, Yiguo Wu, Lu Zhao, Lamei Yu","doi":"10.1615/jenvironpatholtoxicoloncol.2024053641","DOIUrl":"https://doi.org/10.1615/jenvironpatholtoxicoloncol.2024053641","url":null,"abstract":"Background: The hypoxic microenvironment of tumor cells is closely related to the progression of ovarian cancer. This study aims to explore the role of matrix-remodeling associated 5 (MXRA5) in regulating cellular hypoxia in ovarian cancer.\u0000Methods: The MXRA5 expression and its clinical significance in ovarian cancer were evaluated using GEPIA2, Kaplan-Meier plotter databases, and immunohistochemistry assay. Ovarian cancer cells were treated with normoxia and hypoxia conditions. The siRNAs were used to knock down the MXRA5 expression in hypoxic cells. The cellular capacities were detected by CCK-8 assay, EdU assay, Transwell invasion assay, and TUNEL assay, respectively.\u0000Results: The MXRA5 level was increased in ovarian cancer and associated with the PFS and survival of ovarian cancer patients. The proliferation and invasion abilities of ovarian cancer cells were promoted, while apoptosis capacities were inhibited in hypoxic cells. After the knockdown of MXRA5 in hypoxic cells, the proliferation and invasion abilities of the cells were reduced, and the apoptosis capacities were enhanced. Moreover, mechanistically, HIF-1α is a key transcription factor in response to hypoxia that binds to the MXRA5 promoter.\u0000Conclusion: MXRA5 expression was induced by hypoxia and had prognostic performance in ovarian cancer. MXRA5 is one of the potential targets of tumor hypoxia regulation. Knockdown of MXRA5 can inhibit proliferation and invasion in ovarian cancer cells caused by HIF-1α, revealing that MXRA5 is one potential targets for tumor hypoxia regulation in ovarian cancer.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"59 6 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141772466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.1615/jenvironpatholtoxicoloncol.2024053872
Meshak Dhanashekaran Cecileya Jasmin, N Radhakrishnan, Venugopal Vinod Prabhu, Kunnathur Murugesan Sakthivel, Rasmi Rajan Radha
The Histone-lysine N-methyltransferase 2D (KMT2D), tumor suppressor gene which is the major component of histone H3K4 mono-methyltransferase in mammals and has significant role in regulation of a gene which are frequently mutated that lead to many different types of cancers that includenon-Hodgkin lymphoma, medulloblastoma, prostate carcinoma, renal carcinoma, bladder carcinoma and lung carcinoma. KMT2D gene epigenetic alterations in histone methylation play a significant role for the initiation and progression of cancers from pre-cancerous lesions, yet its complete function in oncogenesis remains unsolved. KMT2D deficiency - loss are thought of initial mediators of cancer development and cell migration such as B‐cell lymphoma, medulloblastoma, melanoma, pancreas and lung cancer. The KMT2D loss has know to activate glycolytic genes that promote aggressive tumor progression. Therefore, the present review serves to underline the update on recent research pertaining toKMT2D gene, that could be a potential therapeutic target in down regulating glycolytic genes such as Pgk1,Ldha, Pgam1 and Gapdh; 2, tyrosine kinases EGFR - ERBB2, RTK-RAS signaling, Ras activator genes Rgl1, Rasgrp1, Rasgrf1, Rasgrf2 and Rapgef5 in suppressing the tumor progression that may represent novel targeted therapy for the management of cancer. This review will facilitate to understand the gene expression that inhibits cancer progression and which could serve as a potential molecular target in understanding cancer pathogenesis.
{"title":"Histone-lysine N-methyltransferase 2D (KMT2D) impending therapeutic target for the management of cancer: The giant rats tail","authors":"Meshak Dhanashekaran Cecileya Jasmin, N Radhakrishnan, Venugopal Vinod Prabhu, Kunnathur Murugesan Sakthivel, Rasmi Rajan Radha","doi":"10.1615/jenvironpatholtoxicoloncol.2024053872","DOIUrl":"https://doi.org/10.1615/jenvironpatholtoxicoloncol.2024053872","url":null,"abstract":"The Histone-lysine N-methyltransferase 2D (KMT2D), tumor suppressor gene which is the major component of histone H3K4 mono-methyltransferase in mammals and has significant role in regulation of a gene which are frequently mutated that lead to many different types of cancers that includenon-Hodgkin lymphoma, medulloblastoma, prostate carcinoma, renal carcinoma, bladder carcinoma and lung carcinoma. KMT2D gene epigenetic alterations in histone methylation play a significant role for the initiation and progression of cancers from pre-cancerous lesions, yet its complete function in oncogenesis remains unsolved. KMT2D deficiency - loss are thought of initial mediators of cancer development and cell migration such as B‐cell lymphoma, medulloblastoma, melanoma, pancreas and lung cancer. The KMT2D loss has know to activate glycolytic genes that promote aggressive tumor progression. Therefore, the present review serves to underline the update on recent research pertaining toKMT2D gene, that could be a potential therapeutic target in down regulating glycolytic genes such as Pgk1,Ldha, Pgam1 and Gapdh; 2, tyrosine kinases EGFR - ERBB2, RTK-RAS signaling, Ras activator genes Rgl1, Rasgrp1, Rasgrf1, Rasgrf2 and Rapgef5 in suppressing the tumor progression that may represent novel targeted therapy for the management of cancer. This review will facilitate to understand the gene expression that inhibits cancer progression and which could serve as a potential molecular target in understanding cancer pathogenesis.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"4 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141744291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.1615/jenvironpatholtoxicoloncol.2024053754
Xiaoping Pu, Hong Wu, Xiaoyan Liu, Fang Yang
Nasopharyngeal carcinoma (NPC) is one of the common malignant tumors in clinic. In the current study, we aim to investigate the effects of PRMT4 on erastin-induced ferroptosis in NPC by cisplatin resistant. PRMT4 expression in patients with Nasopharyngeal carcinoma by cisplatin was up-regulated. PRMT4 up-regulation promoted cell growth of erastin-induced ferroptosis in Nasopharyngeal carcinoma cisplatin resistant cells. PRMT4 down-regulation reduced cell growth of erastin-induced ferroptosis in Nasopharyngeal carcinoma cisplatin resistant cells. PRMT4 promoted Tumor volume in mice model of erastin-induced Nasopharyngeal carcinoma by cisplatin. PRMT4 up-regulation reduced erastin-induced ferroptosis in Nasopharyngeal carcinoma cisplatin resistant cells by Mitochondrial damage. PRMT4 up-regulation induced Nrf2 protein expression in model of erastin-induced Nasopharyngeal carcinoma by cisplatin. Nrf2 reduced the effects of si-PRMT4 on cell growth of erastin-induced ferroptosis in Nasopharyngeal carcinoma cisplatin resistant cells. Nrf2 inhibitor reduced the effects of PRMT4 on cell growth of erastin-induced ferroptosis in Nasopharyngeal carcinoma cisplatin resistant cells. Nrf2 reduced the effects of si-PRMT4 on erastin-induced ferroptosis in Nasopharyngeal carcinoma cisplatin resistant cells by Mitochondrial damage. PRMT4 protein interlinked with Nrf2 protein to decrease Nrf2 Ubiquitination. Methylation increased PRMT4 DNA stability. Collectively, our data reveal that PRMT4 reduced erastin-induced ferroptosis in Nasopharyngeal carcinoma cisplatin resistant cells by Nrf2/GPX4 pathway, suggesting that targeting PRMT4 may present as a potential strategy against the develo
{"title":"PRMT4 reduced erastin-induced ferroptosis in Nasopharyngeal carcinoma cisplatin resistant cells by Nrf2/GPX4 pathway","authors":"Xiaoping Pu, Hong Wu, Xiaoyan Liu, Fang Yang","doi":"10.1615/jenvironpatholtoxicoloncol.2024053754","DOIUrl":"https://doi.org/10.1615/jenvironpatholtoxicoloncol.2024053754","url":null,"abstract":"Nasopharyngeal carcinoma (NPC) is one of the common malignant tumors in clinic. In the current study, we aim to investigate the effects of PRMT4 on erastin-induced ferroptosis in NPC by cisplatin resistant.\u0000PRMT4 expression in patients with Nasopharyngeal carcinoma by cisplatin was up-regulated. PRMT4 up-regulation promoted cell growth of erastin-induced ferroptosis in Nasopharyngeal carcinoma cisplatin resistant cells. PRMT4 down-regulation reduced cell growth of erastin-induced ferroptosis in Nasopharyngeal carcinoma cisplatin resistant cells. PRMT4 promoted Tumor volume in mice model of erastin-induced Nasopharyngeal carcinoma by cisplatin. PRMT4 up-regulation reduced erastin-induced ferroptosis in Nasopharyngeal carcinoma cisplatin resistant cells by Mitochondrial damage. PRMT4 up-regulation induced Nrf2 protein expression in model of erastin-induced Nasopharyngeal carcinoma by cisplatin. Nrf2 reduced the effects of si-PRMT4 on cell growth of erastin-induced ferroptosis in Nasopharyngeal carcinoma cisplatin resistant cells. Nrf2 inhibitor reduced the effects of PRMT4 on cell growth of erastin-induced ferroptosis in Nasopharyngeal carcinoma cisplatin resistant cells. Nrf2 reduced the effects of si-PRMT4 on erastin-induced ferroptosis in Nasopharyngeal carcinoma cisplatin resistant cells by Mitochondrial damage. PRMT4 protein interlinked with Nrf2 protein to decrease Nrf2 Ubiquitination. Methylation increased PRMT4 DNA stability.\u0000Collectively, our data reveal that PRMT4 reduced erastin-induced ferroptosis in Nasopharyngeal carcinoma cisplatin resistant cells by Nrf2/GPX4 pathway, suggesting that targeting PRMT4 may present as a potential strategy against the develo","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"28 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141772319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01DOI: 10.1615/jenvironpatholtoxicoloncol.2024052414
Hoang Ngau Tran, Chau Phan Kim Dieu, Chau Phan Kim Hue, Duyen Thi Ngoc Huynh, Bich Hang Do, Luong Do Huynh
Ginseng is considered as a beneficial herbal remedy and over recent years its efficacy and safety have been verified in clinical therapy. There are two typical species of ginseng concluding Asian and American ginseng. The varieties of both species have been applied for commercialized materials in different stages of processing from raw to processed products. Panax vietnamensis Ha et Grushv. (P. vietnamensis) belongs to Asian ginseng and has been utilized as effective herbal remedy. P. vietnamensis is believed to improve immune response, longevity and consequent health. There are more than 300 bioactive compounds have been isolated from P. vietnamensis and classified in various groups, such as ginsenosides, flavonoids, phenolics .... These biological activities consist of anti-tumor, anti-inflammatory, neuroprotective and anti-stress, which are validated by in vitro and in vivo studies. In this review, we systematize the literatures about ethnopharmacology, major bioactive constituents, and toxicology of P. vietnamensis, which were certified by various studies. Furthermore, we also summarize the current method to micro-propagate and lists of extracting sources of bioactive compounds (root, leave, stem…) and the solvents deployed during extraction process. Therefore, this review would provide the firm-evidences and premise for the therapeutic potentials of P. vietnamensis in the future.
人参被认为是一种有益的草药，近年来，其疗效和安全性已在临床治疗中得到验证。人参有两个典型品种，即亚洲人参和西洋参。这两个品种的人参在从原料到加工产品的不同加工阶段都被用作商业化材料。越南人参（Panax vietnamensis Ha et Grushv.(P. vietnamensis) 属于亚洲人参，一直被用作有效的草药。人们认为越南三七能提高免疫反应，延年益寿，从而促进健康。目前已从越南刺五加中分离出 300 多种生物活性化合物，分为人参皂苷、黄酮类、酚类 .... 等不同类别。这些生物活性包括抗肿瘤、抗炎、神经保护和抗应激，并通过体外和体内研究得到了验证。在这篇综述中，我们系统梳理了有关越南罂粟的民族药理学、主要生物活性成分和毒理学的文献，这些文献已得到各种研究的证实。此外，我们还总结了当前的微繁殖方法、生物活性化合物的提取来源（根、叶、茎......）清单以及提取过程中使用的溶剂。因此，这篇综述将为越南鹅掌楸未来的治疗潜力提供可靠的证据和前提。
{"title":"Ethnopharmacology, genetic diversity, phytochemistry and pharmacological effects of Panax vietnamensis Ha et Grushv.: A review","authors":"Hoang Ngau Tran, Chau Phan Kim Dieu, Chau Phan Kim Hue, Duyen Thi Ngoc Huynh, Bich Hang Do, Luong Do Huynh","doi":"10.1615/jenvironpatholtoxicoloncol.2024052414","DOIUrl":"https://doi.org/10.1615/jenvironpatholtoxicoloncol.2024052414","url":null,"abstract":"Ginseng is considered as a beneficial herbal remedy and over recent years its efficacy and safety have been verified in clinical therapy. There are two typical species of ginseng concluding Asian and American ginseng. The varieties of both species have been applied for commercialized materials in different stages of processing from raw to processed products. Panax vietnamensis Ha et Grushv. (P. vietnamensis) belongs to Asian ginseng and has been utilized as effective herbal remedy. P. vietnamensis is believed to improve immune response, longevity and consequent health. There are more than 300 bioactive compounds have been isolated from P. vietnamensis and classified in various groups, such as ginsenosides, flavonoids, phenolics .... These biological activities consist of anti-tumor, anti-inflammatory, neuroprotective and anti-stress, which are validated by in vitro and in vivo studies. In this review, we systematize the literatures about ethnopharmacology, major bioactive constituents, and toxicology of P. vietnamensis, which were certified by various studies. Furthermore, we also summarize the current method to micro-propagate and lists of extracting sources of bioactive compounds (root, leave, stem…) and the solvents deployed during extraction process. Therefore, this review would provide the firm-evidences and premise for the therapeutic potentials of P. vietnamensis in the future.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"167 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141505467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01DOI: 10.1615/jenvironpatholtoxicoloncol.2024053645
Bo Ren, Chunyue Zhang, Yanmin Zhang, Yan Wang, Liyan Liu
Background: Gastric cancer has become a great challenge to human health in the world. We studied the expression and role of circ_0001438 with the aim of finding a biomarker to assess the prognosis of gastric cancer. Methods: Through a polymerase chain reaction, circ_0001438 expression in gastric cancer was detected. Chi-square test, multi-factor Cox regression, and Kaplan-Meier analyses were used to determine the association between circ_0001438 and the patients' clinical condition and prognosis. Using the luciferase reporter gene system, the interaction between circ_0001438 and miR-1290 was analyzed, and the regulatory impact of circ_0001438/miR-1290 on the activity of gastric cancer cells was examined flowing the Transwell assay and CCK8 assay. Results: In gastric cancer tissues and cells, circ_0001438 expression was downregulated, and miR-1290 expression was upregulated and the two were negatively correlated. miR-1290 inhibitors were transfected and significantly increased the activity of circ_0001438 luciferase, while miR-1290 mimics decreased the activity. Overexpression of circ_0001438 decreased miR-1290 expression and inhibited the proliferation and metastasis of gastric cancer cells, which was reversed when miR-1290 mimics were transfected. Additionally, there was a correlation between circ_0001438 expression and lymph node metastases, tumor size, and TNM stage of gastric cancer. Low circ_0001438 expression predicts poor prognosis of gastric cancer patients. Conclusions: Circ_0001438 is a biomarker for tumor development and clinical prognosis in gastric cancer. It works by downregulating miR-1290 to control the activity of gastric cancer cells.
{"title":"Expression and significance of circ_0001438 in development of gastric cancer","authors":"Bo Ren, Chunyue Zhang, Yanmin Zhang, Yan Wang, Liyan Liu","doi":"10.1615/jenvironpatholtoxicoloncol.2024053645","DOIUrl":"https://doi.org/10.1615/jenvironpatholtoxicoloncol.2024053645","url":null,"abstract":"Background: Gastric cancer has become a great challenge to human health in the world. We studied the expression and role of circ_0001438 with the aim of finding a biomarker to assess the prognosis of gastric cancer.\u0000Methods: Through a polymerase chain reaction, circ_0001438 expression in gastric cancer was detected. Chi-square test, multi-factor Cox regression, and Kaplan-Meier analyses were used to determine the association between circ_0001438 and the patients' clinical condition and prognosis. Using the luciferase reporter gene system, the interaction between circ_0001438 and miR-1290 was analyzed, and the regulatory impact of circ_0001438/miR-1290 on the activity of gastric cancer cells was examined flowing the Transwell assay and CCK8 assay.\u0000Results: In gastric cancer tissues and cells, circ_0001438 expression was downregulated, and miR-1290 expression was upregulated and the two were negatively correlated. miR-1290 inhibitors were transfected and significantly increased the activity of circ_0001438 luciferase, while miR-1290 mimics decreased the activity. Overexpression of circ_0001438 decreased miR-1290 expression and inhibited the proliferation and metastasis of gastric cancer cells, which was reversed when miR-1290 mimics were transfected. Additionally, there was a correlation between circ_0001438 expression and lymph node metastases, tumor size, and TNM stage of gastric cancer. Low circ_0001438 expression predicts poor prognosis of gastric cancer patients.\u0000Conclusions: Circ_0001438 is a biomarker for tumor development and clinical prognosis in gastric cancer. It works by downregulating miR-1290 to control the activity of gastric cancer cells.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"15 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141505466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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