Diagnostic Value of lncRNA XIST in Saliva for Early Peri-Implantitis.

IF 1.4 4区医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Oral health & preventive dentistry Pub Date : 2024-08-06 DOI:10.3290/j.ohpd.b5656312

Mei Chai, Jinzhong Zhang, Qianjiao Meng, Andong Liu

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Abstract

Purpose: To analyse the relative expression and diagnostic potential of lncRNA XIST (XIST) in peri-implantitis, and explore the related mechanism of XIST in peri-implantitis.

Materials and methods: XIST expression in saliva of patients with peri-implantitis was detected by qRT-PCR. The diagnostic significance of XIST in peri-implantitis was assessed by ROC curve. Clinical indicators of the included patients were collected and the correlation between XIST levels and peri-implant indicators was determined by Pearson correlation analysis. Bioinformatic prediction and luciferase reporter assay confirmed the targeting relationship of XIST with downstream factors.

Results: Salivary XIST levels were obviously higher in patients with peri-implantitis than in the healthy control group, and the AUC value for identifying patients was 0.8742 with a sensitivity and specificity of 83.5% and 81.4%. Patients in the peri-implantitis group had higher levels of plaque index (PLI), sulcus bleeding index (SBI) and probing depth (PD) than those in the healthy control group, and the expression of XIST was positively correlated with PLI, SBI, and PD levels. In addition, miR-150-5p was confirmed to be a potential downstream target of XIST.

Conclusion: XIST was overexpressed in the saliva of patients with peri-implantitis and correlated with the severity of the disease. XIST has high diagnostic significance for detecting peri-implantitis.

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唾液中的 lncRNA XIST 对早期种植体周围炎的诊断价值

目的：分析lncRNA XIST（XIST）在种植体周围炎中的相对表达和诊断潜力，并探讨XIST在种植体周围炎中的相关机制：采用qRT-PCR方法检测种植体周围炎患者唾液中XIST的表达。通过ROC曲线评估XIST在种植体周围炎中的诊断意义。收集纳入患者的临床指标，并通过皮尔逊相关分析确定 XIST 水平与种植体周围指标之间的相关性。生物信息学预测和荧光素酶报告实验证实了XIST与下游因素的靶向关系：结果：种植体周围炎患者唾液中的 XIST 水平明显高于健康对照组，识别患者的 AUC 值为 0.8742，灵敏度和特异度分别为 83.5% 和 81.4%。与健康对照组相比，种植体周围炎组患者的斑块指数（PLI）、沟出血指数（SBI）和探查深度（PD）水平更高，而 XIST 的表达与 PLI、SBI 和 PD 水平呈正相关。此外，miR-150-5p被证实是XIST的潜在下游靶点：结论：XIST在种植体周围炎患者的唾液中过度表达，并与疾病的严重程度相关。XIST对检测种植体周围炎具有很高的诊断意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral health & preventive dentistry DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Clinicians, general practitioners, teachers, researchers, and public health administrators will find this journal an indispensable source of essential, timely information about scientific progress in the fields of oral health and the prevention of caries, periodontal diseases, oral mucosal diseases, and dental trauma. Central topics, including oral hygiene, oral epidemiology, oral health promotion, and public health issues, are covered in peer-reviewed articles such as clinical and basic science research reports; reviews; invited focus articles, commentaries, and guest editorials; and symposium, workshop, and conference proceedings.