A robust paradigm for studying regeneration after traumatic spinal cord injury in zebrafish

IF 2.7 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of Neuroscience Methods Pub Date : 2024-08-06 DOI:10.1016/j.jneumeth.2024.110243

Gentry Andrews , Geoffrey Andrews , Yuk Fai Leung , Daniel M. Suter

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Abstract

Background

Zebrafish are vertebrates with a high potential of regeneration after injury in the central nervous system. Therefore, they have emerged as a useful model system for studying traumatic spinal cord injuries.

New Method

Using larval zebrafish, we have developed a robust paradigm to model the effects of anterior spinal cord injury, which correspond to the debilitating injuries of the cervical and thoracic regions in humans. Our new paradigm consists of a more anterior injury location compared to previous studies, a modified behavioral assessment using the visual motor response, and a new data analysis code.

Results

Our approach enables a spinal cord injury closer to the hindbrain with more functional impact compared to previous studies using a more posterior injury location. Results reported in this work reveal recovery over seven days following spinal cord injury.

Comparing with existing Methods

The present work describes a modified paradigm for the in vivo study of spinal cord regeneration after injury using larval zebrafish, including an anterior injury location, a robust behavioral assessment, and a new data analysis software.

Conclusions

Our findings lay the foundation for applying this paradigm to study the effects of drugs, nutrition, and other treatments to improve the regeneration process.

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研究斑马鱼创伤性脊髓损伤后再生的可靠范例

背景：斑马鱼是脊椎动物，中枢神经系统损伤后再生潜力很大。因此，斑马鱼已成为研究创伤性脊髓损伤的有用模型系统：新方法：我们利用幼体斑马鱼开发了一种稳健的模式来模拟脊髓前部损伤的影响，这种损伤与人类颈椎和胸椎区域的衰弱性损伤相对应。与之前的研究相比，我们的新范例包括更靠前的损伤位置、使用视觉运动反应的改良行为评估以及新的数据分析代码：结果：与之前使用较后损伤位置的研究相比，我们的方法使脊髓损伤更接近后脑，对功能影响更大。本研究报告的结果表明，脊髓损伤后七天内即可恢复：与现有方法的比较：本研究描述了一种使用幼体斑马鱼进行体内脊髓损伤后再生研究的改进范式，包括前部损伤位置、稳健的行为评估和新的数据分析软件：我们的研究结果为应用该范例研究药物、营养和其他治疗方法对改善再生过程的影响奠定了基础。

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来源期刊

Journal of Neuroscience Methods 医学-神经科学

CiteScore

7.10

自引率

3.30%

发文量

226

审稿时长

52 days

期刊介绍： The Journal of Neuroscience Methods publishes papers that describe new methods that are specifically for neuroscience research conducted in invertebrates, vertebrates or in man. Major methodological improvements or important refinements of established neuroscience methods are also considered for publication. The Journal''s Scope includes all aspects of contemporary neuroscience research, including anatomical, behavioural, biochemical, cellular, computational, molecular, invasive and non-invasive imaging, optogenetic, and physiological research investigations.