3D Modeling of Cells of the Human Respiratory System for Studying the Prooxidant, Proapoptotic, and Profibrinogenic Effects of Carbon Nanotubes

IF 0.8 Q3 Engineering Nanotechnologies in Russia Pub Date : 2024-08-06 DOI:10.1134/S2635167624600342
L. M. Fatkhutdinova, G. F. Gabidinova, G. A. Timerbulatova, E. V. Valeeva, I. V. Kosyeva, E. V. Ubeykina, A. A. Sayagfarova
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Abstract

The concept of “3Rs” (refinement, reduction, and replacement) has become widespread in toxicological studies. 3D cell models are a further development of traditional 2D models. In the current study, 3D models of the human respiratory system consisting of BEAS-2B bronchial epithelial cells and MRC5-SV40 lung fibroblasts are developed. The results of studying the toxicity of carbon nanotubes (CNTs) using advanced 3D models demonstrate the absence of proapoptotic and profibrogenic effects at concentrations corresponding to the reference exposure level. However, starting at a CNT concentration of 20 μg/mL, well above concentrations corresponding to the reference exposure level, signs of oxidative stress are detected in the cell models. 3D cell models can be recommended as a more objective screening method for assessing the toxicity of CNTs when moving from traditional in vitro experiments to in vivo studies.

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用于研究碳纳米管促氧化、促凋亡和促纤维蛋白生成效应的人体呼吸系统细胞三维模型
摘要 "3R"(改进、减少和替换)的概念已在毒理学研究中得到广泛应用。三维细胞模型是传统二维模型的进一步发展。本研究建立了由 BEAS-2B 支气管上皮细胞和 MRC5-SV40 肺成纤维细胞组成的人体呼吸系统三维模型。利用先进的三维模型研究碳纳米管(CNTs)毒性的结果表明,在与参考暴露水平相对应的浓度下,碳纳米管(CNTs)不存在促凋亡和促嗜铬细胞生成效应。然而,从碳纳米管浓度为 20 μg/mL(远高于参考暴露水平)开始,细胞模型中检测到氧化应激迹象。当从传统的体外实验转向体内研究时,三维细胞模型可作为评估 CNT 毒性的一种更客观的筛选方法。
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来源期刊
Nanotechnologies in Russia
Nanotechnologies in Russia NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
1.20
自引率
0.00%
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0
期刊介绍: Nanobiotechnology Reports publishes interdisciplinary research articles on fundamental aspects of the structure and properties of nanoscale objects and nanomaterials, polymeric and bioorganic molecules, and supramolecular and biohybrid complexes, as well as articles that discuss technologies for their preparation and processing, and practical implementation of products, devices, and nature-like systems based on them. The journal publishes original articles and reviews that meet the highest scientific quality standards in the following areas of science and technology studies: self-organizing structures and nanoassemblies; nanostructures, including nanotubes; functional and structural nanomaterials; polymeric, bioorganic, and hybrid nanomaterials; devices and products based on nanomaterials and nanotechnology; nanobiology and genetics, and omics technologies; nanobiomedicine and nanopharmaceutics; nanoelectronics and neuromorphic computing systems; neurocognitive systems and technologies; nanophotonics; natural science methods in a study of cultural heritage items; metrology, standardization, and monitoring in nanotechnology.
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