L. M. Fatkhutdinova, G. F. Gabidinova, G. A. Timerbulatova, E. V. Valeeva, I. V. Kosyeva, E. V. Ubeykina, A. A. Sayagfarova
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引用次数: 0
Abstract
The concept of “3Rs” (refinement, reduction, and replacement) has become widespread in toxicological studies. 3D cell models are a further development of traditional 2D models. In the current study, 3D models of the human respiratory system consisting of BEAS-2B bronchial epithelial cells and MRC5-SV40 lung fibroblasts are developed. The results of studying the toxicity of carbon nanotubes (CNTs) using advanced 3D models demonstrate the absence of proapoptotic and profibrogenic effects at concentrations corresponding to the reference exposure level. However, starting at a CNT concentration of 20 μg/mL, well above concentrations corresponding to the reference exposure level, signs of oxidative stress are detected in the cell models. 3D cell models can be recommended as a more objective screening method for assessing the toxicity of CNTs when moving from traditional in vitro experiments to in vivo studies.
期刊介绍:
Nanobiotechnology Reports publishes interdisciplinary research articles on fundamental aspects of the structure and properties of nanoscale objects and nanomaterials, polymeric and bioorganic molecules, and supramolecular and biohybrid complexes, as well as articles that discuss technologies for their preparation and processing, and practical implementation of products, devices, and nature-like systems based on them. The journal publishes original articles and reviews that meet the highest scientific quality standards in the following areas of science and technology studies: self-organizing structures and nanoassemblies; nanostructures, including nanotubes; functional and structural nanomaterials; polymeric, bioorganic, and hybrid nanomaterials; devices and products based on nanomaterials and nanotechnology; nanobiology and genetics, and omics technologies; nanobiomedicine and nanopharmaceutics; nanoelectronics and neuromorphic computing systems; neurocognitive systems and technologies; nanophotonics; natural science methods in a study of cultural heritage items; metrology, standardization, and monitoring in nanotechnology.