Both Th1 and Th2 CD4 + T-Cell Lineage Infiltrations Decrease in Post-hematopoietic Stem Cell Transplantation Colon Adenoma.

IF 1.6 Q4 ONCOLOGY Journal of Gastrointestinal Cancer Pub Date : 2024-12-01 Epub Date: 2024-08-19 DOI:10.1007/s12029-024-01097-5
Yasuo Matsubara, Yasunori Ota, Tamami Denda, Yukihisa Tanaka, Masamichi Isobe, Seiko Kato, Takaaki Konuma, Satoshi Takahashi, Yoshihiro Hirata, Hiroaki Ikematsu, Keisuke Baba, Narikazu Boku
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Abstract

Purpose: As long-term survival improves after allogeneic hematopoietic stem cell transplantation (HSCT), the risk for secondary solid cancers, including colon cancer, also increases. However, the pathogenesis of secondary solid cancers in post-HSCT patients remains unclear. This study aimed to investigate the involvement of local immunity in colon carcinogenesis in post-HSCT patients by assessing the infiltrating T cells in colon adenomas as premalignant lesions of colon cancer in adenoma-carcinoma sequence.

Methods: Colon adenoma samples obtained from 19 post-HSCT patients and 57 non-HSCT participants were analyzed via immunohistochemistry. Double staining of CD4/T-bet, CD4/GATA3, and CD4/FoxP3 was performed for evaluation of helper T-cell lineages (Th1, Th2, and regulatory T cells, respectively) and CD8 staining for CD8+ T cells.

Results: There were no significant between-group differences in the number of infiltrating CD4+ T cells and CD8+ T cells in adenomas. However, the number of both CD4+/T-bet+ and CD4+/GATA3+ T cells was significantly lower in the post-HSCT adenomas than in the non-HSCT adenomas (P = 0.0171 and 0.0009, respectively), whereas no significant differences were found in the number of CD4+/FoxP3+ cells.

Conclusion: Although the number of infiltrating CD4+ and CD8+ T cells, and even Treg cell counts, is sufficiently recovered post-HSCT, CD4+ T-cell dysfunction due to suppressed activation and differentiation in colon adenomas might be involved in colon carcinogenesis in post-HSCT patients. Elucidating the pathogenesis will contribute to the development of effective screening and prevention programs for secondary colon cancer in post-HSCT patients.

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造血干细胞移植后结肠腺瘤中 Th1 和 Th2 CD4 + T 细胞系浸润均减少
目的:随着异基因造血干细胞移植(HSCT)后长期存活率的提高,继发性实体癌(包括结肠癌)的风险也随之增加。然而,造血干细胞移植后患者继发实体癌的发病机制仍不清楚。本研究旨在通过评估作为结肠癌癌前病变的结肠腺瘤中浸润的 T 细胞,研究局部免疫参与造血干细胞移植后患者结肠癌发生的机制:通过免疫组化方法分析了19例HSCT后患者和57例非HSCT参与者的结肠腺瘤样本。对 CD4/T-bet、CD4/GATA3 和 CD4/FoxP3 进行双重染色,以评估辅助性 T 细胞系(分别为 Th1、Th2 和调节性 T 细胞),并对 CD8+ T 细胞进行 CD8 染色:结果:腺瘤中浸润的 CD4+ T 细胞和 CD8+ T 细胞的数量在组间无明显差异。然而,HSCT 后腺瘤中 CD4+/T-bet+ 和 CD4+/GATA3+ T 细胞的数量明显低于非 HSCT 腺瘤(P = 0.0171 和 0.0009,分别为 0.0171 和 0.0009),而 CD4+/FoxP3+ 细胞的数量则无明显差异:结论:虽然HSCT术后浸润的CD4+和CD8+T细胞数量,甚至Treg细胞数量都得到了充分恢复,但结肠腺瘤中CD4+T细胞的活化和分化受抑制导致的功能障碍可能与HSCT术后患者的结肠癌发生有关。阐明其发病机制将有助于制定有效的筛查和预防方案,预防 HSCT 后患者的继发性结肠癌。
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来源期刊
CiteScore
3.80
自引率
0.00%
发文量
121
期刊介绍: The Journal of Gastrointestinal Cancer is a multidisciplinary medium for the publication of novel research pertaining to cancers arising from the gastrointestinal tract.The journal is dedicated to the most rapid publication possible.The journal publishes papers in all relevant fields, emphasizing those studies that are helpful in understanding and treating cancers affecting the esophagus, stomach, liver, gallbladder and biliary tree, pancreas, small bowel, large bowel, rectum, and anus. In addition, the Journal of Gastrointestinal Cancer publishes basic and translational scientific information from studies providing insight into the etiology and progression of cancers affecting these organs. New insights are provided from diverse areas of research such as studies exploring pre-neoplastic states, risk factors, epidemiology, genetics, preclinical therapeutics, surgery, radiation therapy, novel medical therapeutics, clinical trials, and outcome studies.In addition to reports of original clinical and experimental studies, the journal also publishes: case reports, state-of-the-art reviews on topics of immediate interest or importance; invited articles analyzing particular areas of pancreatic research and knowledge; perspectives in which critical evaluation and conflicting opinions about current topics may be expressed; meeting highlights that summarize important points presented at recent meetings; abstracts of symposia and conferences; book reviews; hypotheses; Letters to the Editors; and other items of special interest, including:Complex Cases in GI Oncology:  This is a new initiative to provide a forum to review and discuss the history and management of complex and involved gastrointestinal oncology cases. The format will be similar to a teaching case conference where a case vignette is presented and is followed by a series of questions and discussion points. A brief reference list supporting the points made in discussion would be expected.
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