{"title":"Partitioning denitrification pathways in N2O emissions from re-flooded dry paddy soils","authors":"Yijia Tang, Budiman Minasny, Alex McBratney","doi":"10.1007/s10533-024-01164-w","DOIUrl":null,"url":null,"abstract":"<div><p>In flooded paddy fields, peak greenhouse gas nitrous oxide (N<sub>2</sub>O) emission after rewetting the dry soils is widely recognised. However, the relative contribution of biotic and abiotic factors to this emission remains uncertain. In this study, we used the isotope technique (δ<sup>18</sup>O and δ<sup>15</sup>N<sup>SP</sup>) and molecular-based microbial analysis in an anoxic incubation experiment to evaluate the contributions of bacterial, fungal, and chemical denitrification to N<sub>2</sub>O emissions. We collected eight representative paddy soils across southern China for an incubation experiment. Results show that during the 10-day incubation period, the net N<sub>2</sub>O emissions were mainly produced by fungal denitrification, which accounted for 58–77% in six of the eight investigated flooded paddy soils. In contrast, bacterial denitrification contributed 6–15% of the net N<sub>2</sub>O emissions. Moreover, around 11–35% of the total N<sub>2</sub>O emissions were derived from chemical denitrification in all soil types. Variation partitioning analysis (VPA) and principal component analysis (PCA) demonstrated that initial soil organic carbon (OC) concentrations were the primary regulator of N<sub>2</sub>O source patterns. Soils with relatively lower OC concentration (7–15 mg g<sup>−1</sup>) tend to be dominated by fungal denitrification, which accounted for the net N<sub>2</sub>O production at the end of the incubation period. Overall, these findings highlight the dominance of the fungal denitrification pathway for N<sub>2</sub>O production in flooded paddy soils, which predominates in soils with relatively lower OC content. This suggests that fungal contribution should be considered when optimizing agricultural management system timing to control N<sub>2</sub>O emissions in flooded paddy soil ecosystems, and for the relevant establishment of predictive numerical models in the future.</p></div>","PeriodicalId":8901,"journal":{"name":"Biogeochemistry","volume":null,"pages":null},"PeriodicalIF":3.9000,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10533-024-01164-w.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biogeochemistry","FirstCategoryId":"93","ListUrlMain":"https://link.springer.com/article/10.1007/s10533-024-01164-w","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
In flooded paddy fields, peak greenhouse gas nitrous oxide (N2O) emission after rewetting the dry soils is widely recognised. However, the relative contribution of biotic and abiotic factors to this emission remains uncertain. In this study, we used the isotope technique (δ18O and δ15NSP) and molecular-based microbial analysis in an anoxic incubation experiment to evaluate the contributions of bacterial, fungal, and chemical denitrification to N2O emissions. We collected eight representative paddy soils across southern China for an incubation experiment. Results show that during the 10-day incubation period, the net N2O emissions were mainly produced by fungal denitrification, which accounted for 58–77% in six of the eight investigated flooded paddy soils. In contrast, bacterial denitrification contributed 6–15% of the net N2O emissions. Moreover, around 11–35% of the total N2O emissions were derived from chemical denitrification in all soil types. Variation partitioning analysis (VPA) and principal component analysis (PCA) demonstrated that initial soil organic carbon (OC) concentrations were the primary regulator of N2O source patterns. Soils with relatively lower OC concentration (7–15 mg g−1) tend to be dominated by fungal denitrification, which accounted for the net N2O production at the end of the incubation period. Overall, these findings highlight the dominance of the fungal denitrification pathway for N2O production in flooded paddy soils, which predominates in soils with relatively lower OC content. This suggests that fungal contribution should be considered when optimizing agricultural management system timing to control N2O emissions in flooded paddy soil ecosystems, and for the relevant establishment of predictive numerical models in the future.
期刊介绍:
Biogeochemistry publishes original and synthetic papers dealing with biotic controls on the chemistry of the environment, or with the geochemical control of the structure and function of ecosystems. Cycles are considered, either of individual elements or of specific classes of natural or anthropogenic compounds in ecosystems. Particular emphasis is given to coupled interactions of element cycles. The journal spans from the molecular to global scales to elucidate the mechanisms driving patterns in biogeochemical cycles through space and time. Studies on both natural and artificial ecosystems are published when they contribute to a general understanding of biogeochemistry.