Hydrogen sulfide inhibits skeletal muscle ageing by up-regulating autophagy through promoting deubiquitination of adenosine 5’-monophosphate (AMP)-activated protein kinase α1 via ubiquitin specific peptidase 5

IF 9.4 1区 医学 Q1 GERIATRICS & GERONTOLOGY Journal of Cachexia Sarcopenia and Muscle Pub Date : 2024-08-27 DOI:10.1002/jcsm.13560
Jia-He Yang, Jun Gao, Ya-Qi E, Li-Jie Jiao, Ren Wu, Qiu-Yi Yan, Zi-Yi Wei, Guo-Liang Yan, Jin-Long Liang, Hong-Zhu Li
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Sodium hydrosulfide (NaHS) was employed as an exogenous donor in the treatment group. The intracellular concentration of H<sub>2</sub>S was quantified by the 7-azido-4-methylcoumarin fluorescence probe. The proteins involved in the ubiquitin-mediated degradation of AMPKα1 were detected by liquid chromatography tandem mass spectrometry (LC–MS/MS) and co-immunoprecipitation (Co-IP). S-sulfhydration of USP5 was tested by a biotin-switch assay. Associated proteins were analysed by western blot.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>NaHS was found to effectively restore the H<sub>2</sub>S content in both ageing gastrocnemius (+91.89%, <i>P</i> &lt; 0.001) and C2C12 myotubes (+27.55%, <i>P</i> &lt; 0.001). In comparison to the D-gal group, NaHS was observed to increase the mean cross-sectional area of muscle fibres (+44.91%, <i>P</i> &lt; 0.001), to decrease the collagen volume fraction of gastrocnemius (−81.32%, <i>P</i> = 0.001) and to reduce the β-galactosidase-positive area of C2C12 myotubes (−28.74%, <i>P</i> &lt; 0.001). NaHS was also found to reverse the expression of muscle atrophy F box protein (MAFbx), muscle-specific RING finger protein 1 (MuRF1), Cyclin D1 and p21 in the ageing gastrocnemius tissue (MAFbx: −31.73%, <i>P</i> = 0.008; MuRF1: −32.37%, <i>P</i> = 0.003; Cyclin D1: +45.34%, <i>P</i> = 0.010; p21: −25.53%, <i>P</i> = 0.022) and C2C12 myotubes (MAFbx: −16.38%, <i>P</i> &lt; 0.001; MuRF1: −16.45%, <i>P</i> = 0.003; Cyclin D1: +40.23%, <i>P</i> &lt; 0.001; p21: −35.85%, <i>P</i> = 0.026). The AMPKα1–ULK1 pathway was activated and autophagy was up-regulated in NaHS-treated gastrocnemius tissue (p-AMPKα1: +61.61%, <i>P</i> = 0.018; AMPKα1: +30.64%, <i>P</i> = 0.010; p-ULK1/ULK1: +85.87%, <i>P</i> = 0.005; p62: −29.07%, <i>P</i> &lt; 0.001; Beclin1: +24.75%, <i>P</i> = 0.007; light chain 3 II/I [LC3 II/I]: +55.78%, <i>P</i> = 0.004) and C2C12 myotubes (p-AMPKα1: +77.49%, <i>P</i> = 0.018; AMPKα1: +26.18%, <i>P</i> = 0.022; p-ULK1/ULK1: +38.34%, <i>P</i> = 0.012; p62: −9.02%, <i>P</i> = 0.014; Beclin1: +13.36%, <i>P</i> &lt; 0.001; LC3 II/I: +79.38%, <i>P</i> = 0.017; autophagy flux: +24.88%, <i>P</i> = 0.034) compared with the <span>D</span>-gal group. The effects of NaHS on autophagy were comparable to those of acadesine and LYN-1604, and chloroquine could reverse its effects on ageing. LC–MS/MS and Co-IP experiments demonstrated that USP5 is a deubiquitinating enzyme of AMPKα1. 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引用次数: 0

Abstract

Background

Hydrogen sulfide (H2S), the third gasotransmitter discovered, regulates a variety of physiological functions. Whether H2S alleviates skeletal muscle ageing by regulating autophagy has not been reported.

Methods

Mice were administered 150 mg/kg/day of D-galactose (D-gal), and C2C12 myotubes were cultured in 20 g/L D-gal to induce ageing. Sodium hydrosulfide (NaHS) was employed as an exogenous donor in the treatment group. The intracellular concentration of H2S was quantified by the 7-azido-4-methylcoumarin fluorescence probe. The proteins involved in the ubiquitin-mediated degradation of AMPKα1 were detected by liquid chromatography tandem mass spectrometry (LC–MS/MS) and co-immunoprecipitation (Co-IP). S-sulfhydration of USP5 was tested by a biotin-switch assay. Associated proteins were analysed by western blot.

Results

NaHS was found to effectively restore the H2S content in both ageing gastrocnemius (+91.89%, P < 0.001) and C2C12 myotubes (+27.55%, P < 0.001). In comparison to the D-gal group, NaHS was observed to increase the mean cross-sectional area of muscle fibres (+44.91%, P < 0.001), to decrease the collagen volume fraction of gastrocnemius (−81.32%, P = 0.001) and to reduce the β-galactosidase-positive area of C2C12 myotubes (−28.74%, P < 0.001). NaHS was also found to reverse the expression of muscle atrophy F box protein (MAFbx), muscle-specific RING finger protein 1 (MuRF1), Cyclin D1 and p21 in the ageing gastrocnemius tissue (MAFbx: −31.73%, P = 0.008; MuRF1: −32.37%, P = 0.003; Cyclin D1: +45.34%, P = 0.010; p21: −25.53%, P = 0.022) and C2C12 myotubes (MAFbx: −16.38%, P < 0.001; MuRF1: −16.45%, P = 0.003; Cyclin D1: +40.23%, P < 0.001; p21: −35.85%, P = 0.026). The AMPKα1–ULK1 pathway was activated and autophagy was up-regulated in NaHS-treated gastrocnemius tissue (p-AMPKα1: +61.61%, P = 0.018; AMPKα1: +30.64%, P = 0.010; p-ULK1/ULK1: +85.87%, P = 0.005; p62: −29.07%, P < 0.001; Beclin1: +24.75%, P = 0.007; light chain 3 II/I [LC3 II/I]: +55.78%, P = 0.004) and C2C12 myotubes (p-AMPKα1: +77.49%, P = 0.018; AMPKα1: +26.18%, P = 0.022; p-ULK1/ULK1: +38.34%, P = 0.012; p62: −9.02%, P = 0.014; Beclin1: +13.36%, P < 0.001; LC3 II/I: +79.38%, P = 0.017; autophagy flux: +24.88%, P = 0.034) compared with the D-gal group. The effects of NaHS on autophagy were comparable to those of acadesine and LYN-1604, and chloroquine could reverse its effects on ageing. LC–MS/MS and Co-IP experiments demonstrated that USP5 is a deubiquitinating enzyme of AMPKα1. Following the knockdown of USP5, the activation of AMPKα1 was decreased (p-AMPKα1: −42.10%, P < 0.001; AMPKα1: −43.93%, P < 0.001), autophagy was inhibited (p-ULK1/ULK1: −27.51, P = 0.001; p62: +36.00, P < 0.001; Beclin1: −22.15%, P < 0.001) and NaHS lost its ability to up-regulate autophagy. NaHS was observed to restore the expression (gastrocnemius: +62.17%, P < 0.001; C2C12 myotubes: +37.51%, P = 0.003) and S-sulfhydration (+53.07%, P = 0.009) of USP5 and reduce the ubiquitination of AMPKα1.

Conclusions

H2S promotes the deubiquitination of AMPKα1 by increasing the expression and S-sulfhydration of USP5, thereby up-regulating autophagy and alleviating skeletal muscle ageing.

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硫化氢通过泛素特异性肽酶 5 促进 5'-单磷酸腺苷(AMP)激活的蛋白激酶 α1的去泛素化,从而上调自噬作用,从而抑制骨骼肌老化。
背景:硫化氢(H2S)是目前发现的第三种气体递质,可调节多种生理功能。H2S是否能通过调节自噬缓解骨骼肌老化,目前还没有相关报道:方法:给小鼠注射 150 毫克/千克/天的 D-半乳糖(D-gal),并在 20 克/升 D-gal 中培养 C2C12 肌管以诱导老化。处理组采用硫氢化钠(NaHS)作为外源供体。细胞内的H2S浓度通过7-叠氮-4-甲基香豆素荧光探针进行量化。液相色谱串联质谱(LC-MS/MS)和共免疫沉淀(Co-IP)法检测了参与泛素介导的 AMPKα1 降解的蛋白质。通过生物素开关检测法检测了 USP5 的 S-硫酸化。相关蛋白通过 Western 印迹进行分析:结果:NaHS 能有效地恢复两种老化腓肠肌中的 H2S 含量(+91.89%,P 结论:H2S 能促进腓肠肌的去 Obubinos 化:H2S通过增加USP5的表达和S-硫酸化来促进AMPKα1的去泛素化,从而上调自噬并缓解骨骼肌老化。
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来源期刊
Journal of Cachexia Sarcopenia and Muscle
Journal of Cachexia Sarcopenia and Muscle MEDICINE, GENERAL & INTERNAL-
CiteScore
13.30
自引率
12.40%
发文量
234
审稿时长
16 weeks
期刊介绍: The Journal of Cachexia, Sarcopenia and Muscle is a peer-reviewed international journal dedicated to publishing materials related to cachexia and sarcopenia, as well as body composition and its physiological and pathophysiological changes across the lifespan and in response to various illnesses from all fields of life sciences. The journal aims to provide a reliable resource for professionals interested in related research or involved in the clinical care of affected patients, such as those suffering from AIDS, cancer, chronic heart failure, chronic lung disease, liver cirrhosis, chronic kidney failure, rheumatoid arthritis, or sepsis.
期刊最新文献
Issue Information Neuromuscular impairment at different stages of human sarcopenia The impact of mobility limitations on geriatric rehabilitation outcomes: Positive effects of resistance exercise training (RESORT) Artificial neural network inference analysis identified novel genes and gene interactions associated with skeletal muscle aging Hydrogen sulfide inhibits skeletal muscle ageing by up-regulating autophagy through promoting deubiquitination of adenosine 5’-monophosphate (AMP)-activated protein kinase α1 via ubiquitin specific peptidase 5
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