Ultrasound Imaging of Macrophages Intracellularly Labelled with Biosynthetic Gas Vesicles.

IF 3 4区医学 Q2 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING Molecular Imaging and Biology Pub Date : 2024-10-01 Epub Date: 2024-08-28 DOI:10.1007/s11307-024-01946-6

Rong Xue, Zhixi Liu, Liang Liu, Shufen Sun, Zheli Gong

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Abstract

Purpose: This study aimed to develop a novel method for real-time imaging to track macrophages and to make it possible to visually track their dynamic features.

Procedures: The archaeon Halobacterium NRC-1 was cultured in an ATCC medium. Buoyant cells were allowed to produce biosynthetic gas vesicles (GVs), and isolated GVs were collected after lysis. Gas vesicle-labelled macrophages (GV@RAWs) were obtained by incubating macrophage (RAW 264.7) cells with GVs. The ability of GV@RAWs to track macrophages in real-time for a long term was assessed using a high-frequency ultrasound imaging system.

Results: We successfully synthesised and isolated GV@RAWs by co-incubating them with RAW 264.7. The results showed that GV@RAW produced significant ultrasound signals without affecting cell survival and could achieve real-time imaging for up to 3 days in vitro.

Conclusion: This research provides a new way to achieve long-term real-time imaging of macrophages, opening up new possibilities for immune response research, clinical diagnosis and therapeutic strategies for inflammatory diseases.

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用生物合成气泡对胞内标记的巨噬细胞进行超声成像

目的：本研究旨在开发一种新的实时成像方法来跟踪巨噬细胞，并使其动态特征可视化成为可能：程序：在 ATCC 培养基中培养古细菌 Halobacterium NRC-1。培养过程：在 ATCC 培养基中培养古细菌 Halobacterium NRC-1，让有浮力的细胞产生生物合成的气泡（GVs），裂解后收集分离的 GVs。将巨噬细胞（RAW 264.7）细胞与 GVs 培养，可获得气泡标记的巨噬细胞（GV@RAWs）。使用高频超声成像系统对 GV@RAWs 长期实时追踪巨噬细胞的能力进行了评估：结果：通过将 GV@RAWs 与 RAW 264.7 共同培养，我们成功合成并分离了 GV@RAWs。结果表明，GV@RAW 在不影响细胞存活的情况下产生了显著的超声信号，并能在体外实现长达 3 天的实时成像：该研究为实现巨噬细胞的长期实时成像提供了一种新方法，为炎症性疾病的免疫反应研究、临床诊断和治疗策略提供了新的可能性。

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来源期刊

Molecular Imaging and Biology 医学-核医学

CiteScore

6.90

自引率

3.20%

发文量

审稿时长

3 months

期刊介绍： Molecular Imaging and Biology (MIB) invites original contributions (research articles, review articles, commentaries, etc.) on the utilization of molecular imaging (i.e., nuclear imaging, optical imaging, autoradiography and pathology, MRI, MPI, ultrasound imaging, radiomics/genomics etc.) to investigate questions related to biology and health. The objective of MIB is to provide a forum to the discovery of molecular mechanisms of disease through the use of imaging techniques. We aim to investigate the biological nature of disease in patients and establish new molecular imaging diagnostic and therapy procedures. Some areas that are covered are: Preclinical and clinical imaging of macromolecular targets (e.g., genes, receptors, enzymes) involved in significant biological processes. The design, characterization, and study of new molecular imaging probes and contrast agents for the functional interrogation of macromolecular targets. Development and evaluation of imaging systems including instrumentation, image reconstruction algorithms, image analysis, and display. Development of molecular assay approaches leading to quantification of the biological information obtained in molecular imaging. Study of in vivo animal models of disease for the development of new molecular diagnostics and therapeutics. Extension of in vitro and in vivo discoveries using disease models, into well designed clinical research investigations. Clinical molecular imaging involving clinical investigations, clinical trials and medical management or cost-effectiveness studies.