Pub Date : 2024-11-04DOI: 10.1007/s11307-024-01960-8
Tukang Peng, Zhijun Li, Jiebing Gao, Min Yang, Yifan Qiu, Jianzhong Xian, Lei Bi, Peizhen Ye, Yongshan Liu, Hongjun Jin
Purpose: Esophageal squamous cell carcinoma (ESCC) frequently exhibits skip metastasis to lymph nodes; however, non-invasive imaging techniques capable of directly visualizing metastatic lymph nodes (MLN) are still lacking. Although biopsy is the clinical standard method, it is invasive and poses risks to patient health. This study aims to detect MLN in an intralymphatic tumor metastasis model of ESCC using the CXCR4-targeted tracer [64Cu]Cu-NOTA-CP01.
Procedures: The CXCR4 expression in ESCC cell lines was assessed using Western blot and immunofluorescence. An intralymphatic tumor metastasis model was established and monitored using bioluminescence imaging (BLI). Small animal PET studies and biodistribution studies were performed to evaluate the specificity of [64Cu]Cu-NOTA-CP01 for MLN. Histopathology evaluation was employed to check for the presence of metastatic tumor cells and to assess CXCR4 expression levels in the metastatic lymph nodes.
Results: The intralymphatic tumor metastasis model was successfully established using the EC109/Luc cell line, which exhibited high CXCR4 expression, as verified by BLI. PET/CT imaging showed that the MLN uptakes in the baseline group were significantly inhibited in the blocking group. The ratios of MLN/muscle and MLN/blood were also significantly higher in the baseline group than in the blocking group. Ex vivo PET/CT imaging of MLN corroborated the in vivo data. Biodistribution studies further supported the PET imaging studies, showing rapid clearance of the tracer from the blood and major organs, with significantly higher MLN/muscle and MLN/blood ratios in the baseline group compared to the blocking group. Histopathological staining verified positive CXCR4 expression in these lymph nodes containing metastatic tumor cells.
Conclusions: Targeting CXCR4 with [64Cu]Cu-NOTA-CP01 for PET imaging of lymph nodes metastasis represents a promising approach that warrants further investigation. These findings have the potential to enhance diagnostic and therapeutic strategies for individuals with lymph nodes metastasis of ESCC.
目的:食管鳞状细胞癌(ESCC)经常出现淋巴结转移,但目前仍缺乏能够直接观察转移淋巴结(MLN)的无创成像技术。虽然活检是临床标准方法,但其具有侵入性,并对患者健康构成风险。本研究旨在利用CXCR4靶向示踪剂[64Cu]Cu-NOTA-CP01.Procedures检测ESCC淋巴内肿瘤转移模型中的MLN:采用Western印迹和免疫荧光评估ESCC细胞系中CXCR4的表达。建立淋巴内肿瘤转移模型,并使用生物发光成像(BLI)进行监测。进行了小动物 PET 研究和生物分布研究,以评估 [64Cu]Cu-NOTA-CP01 对 MLN 的特异性。组织病理学评估用于检查转移性肿瘤细胞的存在,并评估转移淋巴结中 CXCR4 的表达水平:结果:使用EC109/Luc细胞系成功建立了淋巴内肿瘤转移模型。PET/CT 成像显示,阻断组明显抑制了基线组的 MLN 摄取。基线组的 MLN/肌肉比率和 MLN/血液比率也明显高于阻断组。MLN 的体外 PET/CT 成像证实了体内数据。生物分布研究进一步支持了 PET 成像研究,显示示踪剂从血液和主要器官中迅速清除,基线组的 MLN/肌肉和 MLN/血液比率明显高于阻断组。组织病理学染色证实,在这些含有转移性肿瘤细胞的淋巴结中,CXCR4呈阳性表达:用[64Cu]Cu-NOTA-CP01靶向CXCR4进行淋巴结转移PET成像是一种很有前景的方法,值得进一步研究。这些发现有望加强对 ESCC 淋巴结转移患者的诊断和治疗策略。
{"title":"In Vivo Detection of Lymph Nodes Metastasis of ESCC Using CXCR4-Targeted Tracer [<sup>64</sup>Cu]Cu-NOTA-CP01.","authors":"Tukang Peng, Zhijun Li, Jiebing Gao, Min Yang, Yifan Qiu, Jianzhong Xian, Lei Bi, Peizhen Ye, Yongshan Liu, Hongjun Jin","doi":"10.1007/s11307-024-01960-8","DOIUrl":"https://doi.org/10.1007/s11307-024-01960-8","url":null,"abstract":"<p><strong>Purpose: </strong>Esophageal squamous cell carcinoma (ESCC) frequently exhibits skip metastasis to lymph nodes; however, non-invasive imaging techniques capable of directly visualizing metastatic lymph nodes (MLN) are still lacking. Although biopsy is the clinical standard method, it is invasive and poses risks to patient health. This study aims to detect MLN in an intralymphatic tumor metastasis model of ESCC using the CXCR4-targeted tracer [<sup>64</sup>Cu]Cu-NOTA-CP01.</p><p><strong>Procedures: </strong>The CXCR4 expression in ESCC cell lines was assessed using Western blot and immunofluorescence. An intralymphatic tumor metastasis model was established and monitored using bioluminescence imaging (BLI). Small animal PET studies and biodistribution studies were performed to evaluate the specificity of [<sup>64</sup>Cu]Cu-NOTA-CP01 for MLN. Histopathology evaluation was employed to check for the presence of metastatic tumor cells and to assess CXCR4 expression levels in the metastatic lymph nodes.</p><p><strong>Results: </strong>The intralymphatic tumor metastasis model was successfully established using the EC109/Luc cell line, which exhibited high CXCR4 expression, as verified by BLI. PET/CT imaging showed that the MLN uptakes in the baseline group were significantly inhibited in the blocking group. The ratios of MLN/muscle and MLN/blood were also significantly higher in the baseline group than in the blocking group. Ex vivo PET/CT imaging of MLN corroborated the in vivo data. Biodistribution studies further supported the PET imaging studies, showing rapid clearance of the tracer from the blood and major organs, with significantly higher MLN/muscle and MLN/blood ratios in the baseline group compared to the blocking group. Histopathological staining verified positive CXCR4 expression in these lymph nodes containing metastatic tumor cells.</p><p><strong>Conclusions: </strong>Targeting CXCR4 with [<sup>64</sup>Cu]Cu-NOTA-CP01 for PET imaging of lymph nodes metastasis represents a promising approach that warrants further investigation. These findings have the potential to enhance diagnostic and therapeutic strategies for individuals with lymph nodes metastasis of ESCC.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-15DOI: 10.1007/s11307-024-01957-3
Junling Li, Huaiyu Zheng, Jenna Olson, Jonathan M Warawa, Chin K Ng
Purpose: Bacterial infection causes significant mortality and morbidity worldwide despite the availability of antibiotics. Differentiation between responders and non-responders early on during antibiotic treatment will be informative to patients and healthcare providers. Our objective was to investigate whether PET imaging with 18F-Fluorodeoxysorbitol (18F-FDS) or 18F-FDG can be used to differentiate responders from non-responders to antibiotic treatment.
Procedures: NTUH-K2044 was used for infection in Albino C57 female mice. Each mouse was inoculated intratracheally with NTUH-K2044 to induce lung infection (n = 8). For treatment studies, two bacterial doses for animal inoculation and two treatment starting times were compared to optimize treatment profiles. 18F-FDS or 8F-FDG /PET imaging was performed to monitor treatment progression.
Results: Our results demonstrated that the treatment profiles for mice infected with 25 CFU hvKp and antibiotic treatment starting at 24 p.i. were not ideal due to no evidence of lung infection and lack of treatment efficacy. The optimal scheme is to use 250 CUF for infection and start antibiotic treatment at 24 h p.i. to monitor antimicrobial efficacy. 75% of the mice were classified as responders to antibiotic treatment. 25% of the mice were classified as non-responders. 18F-FDG was used to compare with 18F-FDS, but all mice showed increased lung uptake of 18F-FDG during 3-day treatments.
Conclusions: 18F-FDS is a promising PET tracer to image bacterial infection. It can be used to monitor response to treatment, and differentiate responders from non-responders to antibiotic treatment, but 18F-FDG cannot, probably due to the presence of high degree of inflammation before and after treatment.
{"title":"Differentiation Between Responders and Non-Responders to Antibiotic Treatment in Mice Using <sup>18</sup>F-Fluorodeoxysorbitol/PET.","authors":"Junling Li, Huaiyu Zheng, Jenna Olson, Jonathan M Warawa, Chin K Ng","doi":"10.1007/s11307-024-01957-3","DOIUrl":"https://doi.org/10.1007/s11307-024-01957-3","url":null,"abstract":"<p><strong>Purpose: </strong>Bacterial infection causes significant mortality and morbidity worldwide despite the availability of antibiotics. Differentiation between responders and non-responders early on during antibiotic treatment will be informative to patients and healthcare providers. Our objective was to investigate whether PET imaging with <sup>18</sup>F-Fluorodeoxysorbitol (<sup>18</sup>F-FDS) or <sup>18</sup>F-FDG can be used to differentiate responders from non-responders to antibiotic treatment.</p><p><strong>Procedures: </strong>NTUH-K2044 was used for infection in Albino C57 female mice. Each mouse was inoculated intratracheally with NTUH-K2044 to induce lung infection (n = 8). For treatment studies, two bacterial doses for animal inoculation and two treatment starting times were compared to optimize treatment profiles. <sup>18</sup>F-FDS or <sup>8</sup>F-FDG /PET imaging was performed to monitor treatment progression.</p><p><strong>Results: </strong>Our results demonstrated that the treatment profiles for mice infected with 25 CFU hvKp and antibiotic treatment starting at 24 p.i. were not ideal due to no evidence of lung infection and lack of treatment efficacy. The optimal scheme is to use 250 CUF for infection and start antibiotic treatment at 24 h p.i. to monitor antimicrobial efficacy. 75% of the mice were classified as responders to antibiotic treatment. 25% of the mice were classified as non-responders. <sup>18</sup>F-FDG was used to compare with <sup>18</sup>F-FDS, but all mice showed increased lung uptake of <sup>18</sup>F-FDG during 3-day treatments.</p><p><strong>Conclusions: </strong><sup>18</sup>F-FDS is a promising PET tracer to image bacterial infection. It can be used to monitor response to treatment, and differentiate responders from non-responders to antibiotic treatment, but <sup>18</sup>F-FDG cannot, probably due to the presence of high degree of inflammation before and after treatment.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-14DOI: 10.1007/s11307-024-01907-z
{"title":"2023 World Molecular Imaging Congress Program.","authors":"","doi":"10.1007/s11307-024-01907-z","DOIUrl":"https://doi.org/10.1007/s11307-024-01907-z","url":null,"abstract":"","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: To evaluate the value of Tc-99m-diethylenetriamine-penta-acetic acid-galactosyl human serum albumin (99mTc-GSA) single photon emission computed tomography (SPECT) for assessing liver fibrosis, and to assess its complementary value to other liver function indices such as fibrosis-4 (FIB-4) index and indocyanine green (ICG) clearance test parameters (ICG-R15 and ICG-K).
Procedures: Seventy-eight patients with chronic liver disease and hepatocellular carcinoma who underwent 99mTc-GSA scintigraphy and other liver function tests including ICG test and FIB-4 index prior to hepatectomy were studied. 99mTc-GSA imaging was performed with SPECT/CT scanner (Discovery NM/CT 670). Immediately after injection of 99mTc-GSA, dynamic imaging was performed for 20 min, followed by SPECT data acquisition for 6 min. LHL15 which is a conventional index by 99mTc-GSA planar images, and liver uptake ration (LUR) was measured from 99mTc-GSA SPECT images. From the liver resection specimens, the degree of liver fibrosis was graded according to the Ludwig scale (F0-4).
Results: Significant differences in LUR, LHL15, ICG-R15, ICG-K, platelet count and FIB-4 index were found between the F0-3 and F4 liver fibrosis patient groups (P < 0.05). Multivariate logistic regression analysis revealed that LUR and ICG-K were independent factors for identifying severe liver fibrosis (F4). Area under the curve of receiver operating curve analysis for the logistic regression model using LUR and ICG-K was 0.83. In the patient group with higher FIB-4 (≥ 3.16), the diagnostic performance of LUR for detecting severe liver fibrosis was significantly better than LHL15 (AUC: 0.83 vs. 0.75, P = 0.048). In the high FIB-4 index group, the sensitivity and specificity for identifying F4 was 88% and 85%, respectively, with LUR cutoff value of 41.2%.
Conclusions: LUR, measured by 99mTc-GSA SPECT, is a useful indicator for identifying sever liver fibrosis. Particularly in patients with high FIB-4 index (≥ 3.16), LUR can be a valuable indicator to identify severe liver fibrosis with high diagnostic accuracy.
{"title":"Utility of Quantitative Assessment of Tc-99m-diethylenetriamine-penta-acetic acid-galactosyl Human Serum Albumin SPECT/CT in the Identification of Severe Liver Fibrosis: Its Complementary Diagnostic Value with Other Liver Function Indices.","authors":"Yoichi Kozaki, Yasutaka Ichikawa, Satoshi Nakamura, Tatsuhiro Kobayashi, Yoya Tomita, Motonori Nagata, Naohisa Kuriyama, Shugo Mizuno, Hajime Sakuma","doi":"10.1007/s11307-024-01958-2","DOIUrl":"https://doi.org/10.1007/s11307-024-01958-2","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the value of Tc-99m-diethylenetriamine-penta-acetic acid-galactosyl human serum albumin (<sup>99m</sup>Tc-GSA) single photon emission computed tomography (SPECT) for assessing liver fibrosis, and to assess its complementary value to other liver function indices such as fibrosis-4 (FIB-4) index and indocyanine green (ICG) clearance test parameters (ICG-R15 and ICG-K).</p><p><strong>Procedures: </strong>Seventy-eight patients with chronic liver disease and hepatocellular carcinoma who underwent <sup>99m</sup>Tc-GSA scintigraphy and other liver function tests including ICG test and FIB-4 index prior to hepatectomy were studied. <sup>99m</sup>Tc-GSA imaging was performed with SPECT/CT scanner (Discovery NM/CT 670). Immediately after injection of <sup>99m</sup>Tc-GSA, dynamic imaging was performed for 20 min, followed by SPECT data acquisition for 6 min. LHL15 which is a conventional index by <sup>99m</sup>Tc-GSA planar images, and liver uptake ration (LUR) was measured from <sup>99m</sup>Tc-GSA SPECT images. From the liver resection specimens, the degree of liver fibrosis was graded according to the Ludwig scale (F0-4).</p><p><strong>Results: </strong>Significant differences in LUR, LHL15, ICG-R15, ICG-K, platelet count and FIB-4 index were found between the F0-3 and F4 liver fibrosis patient groups (P < 0.05). Multivariate logistic regression analysis revealed that LUR and ICG-K were independent factors for identifying severe liver fibrosis (F4). Area under the curve of receiver operating curve analysis for the logistic regression model using LUR and ICG-K was 0.83. In the patient group with higher FIB-4 (≥ 3.16), the diagnostic performance of LUR for detecting severe liver fibrosis was significantly better than LHL15 (AUC: 0.83 vs. 0.75, P = 0.048). In the high FIB-4 index group, the sensitivity and specificity for identifying F4 was 88% and 85%, respectively, with LUR cutoff value of 41.2%.</p><p><strong>Conclusions: </strong>LUR, measured by <sup>99m</sup>Tc-GSA SPECT, is a useful indicator for identifying sever liver fibrosis. Particularly in patients with high FIB-4 index (≥ 3.16), LUR can be a valuable indicator to identify severe liver fibrosis with high diagnostic accuracy.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142400764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-04DOI: 10.1007/s11307-024-01950-w
Won-Gun Yun, Joonhyung Gil, Hongyoon Choi, Youngmin Han, Hye-Sol Jung, Young Jae Cho, Minseok Suh, Wooil Kwon, Yun-Sang Lee, Gi Jeong Cheon, Jin-Young Jang
Purpose: Accurate clinical staging of potentially resectable pancreatic ductal adenocarcinoma (PDAC) is critical for establishing optimal treatment strategies. While the efficacy of fluorine-18-fluorodeoxyglucose ([18F]FDG) positron emission tomography/computed tomography (PET/CT) in clinical staging is unclear, PET/CT detecting fibroblast-activation protein (FAP) expression has recently received considerable attention for detecting various tumors, including PDAC, with high sensitivity. We explored the efficacy of [18F]FDG and [18F]AIF-FAPI-74 PET/CT in the initial evaluation of potentially resectable PDAC.
Procedures: Between 2021 and 2022, twenty participants with newly diagnosed potentially resectable PDAC were enrolled. After the initial evaluation with pancreatic CT, [18F]FDG PET/CT, and [18F]AIF-FAPI-74 PET/CT, treatment strategies were determined considering the participant's general status, clinical staging, and resectability. Pathological information from the surgical specimens was only available in 17 participants who underwent curative-intent surgery. Head-to-head comparisons of quantitative radiotracer uptake and diagnostic performance were performed among imaging modalities.
Results: [18F]AIF-FAPI-74 PET/CT showed a significantly higher maximum standardized uptake value than [18F]FDG PET/CT did in evaluating primary pancreatic lesions (median [interquartile range]; 12.6 [10.7-13.7] vs. 6.3 [4.8-9.2]; P < 0.001). In contrast, [18F]AIF-FAPI-74 PET/CT showed a significantly lower mean standardized uptake value than [18F]FDG PET/CT did in evaluating background organ (median [interquartile range]) 0.8 [0.7-0.9] vs. 2.6 [2.3-2.7]; P < 0.001). In addition, the sensitivity of [18F]AIF-FAPI-74 PET/CT in detecting metastatic lymph nodes was higher than that of [18F]FDG PET/CT (50.0% vs. 0.0%; P = 0.026).
Conclusion: This study demonstrated that [18F]AIF-FAPI-74 PET/CT could improve the clinical staging of potentially resectable PDAC.
目的:对可能切除的胰腺导管腺癌(PDAC)进行准确的临床分期对于制定最佳治疗策略至关重要。虽然氟-18-氟脱氧葡萄糖([18F]FDG)正电子发射断层扫描/计算机断层扫描(PET/CT)在临床分期中的疗效尚不明确,但检测成纤维细胞活化蛋白(FAP)表达的 PET/CT 最近在高灵敏度检测包括 PDAC 在内的各种肿瘤方面受到了广泛关注。我们探讨了[18F]FDG和[18F]AIF-FAPI-74 PET/CT在初步评估潜在可切除PDAC中的疗效:2021年至2022年间,20名新确诊的潜在可切除PDAC患者入组。通过胰腺 CT、[18F]FDG PET/CT 和 [18F]AIF-FAPI-74 PET/CT 进行初步评估后,根据参与者的一般状况、临床分期和可切除性确定治疗策略。只有17名接受了根治性手术的患者可以获得手术标本的病理信息。对不同成像模式的放射性示踪剂定量摄取和诊断性能进行了头对头比较:结果:在评估原发性胰腺病变时,[18F]AIF-FAPI-74 PET/CT 显示的最大标准化摄取值明显高于[18F]FDG PET/CT (中位数[四分位间范围];12.6 [10.7-13.7] vs. 6.3 [4.8-9.2]; P 18F]AIF-FAPI-74 PET/CT 在评估背景器官时显示的平均标准化摄取值明显低于[18F]FDG PET/CT(中位[四分位间范围])0.8 [0.7-0.9] vs. 2.6 [2.3-2.7];18F]AIF-FAPI-74 PET/CT 检测转移淋巴结的平均标准化摄取值高于[18F]FDG PET/CT(50.0% vs. 0.0%;P = 0.026):本研究表明,[18F]AIF-FAPI-74 PET/CT 可改善潜在可切除 PDAC 的临床分期。
{"title":"Prospective Comparison of [<sup>18</sup>F]FDG and [<sup>18</sup>F]AIF-FAPI-74 PET/CT in the Evaluation of Potentially Resectable Pancreatic Ductal Adenocarcinoma.","authors":"Won-Gun Yun, Joonhyung Gil, Hongyoon Choi, Youngmin Han, Hye-Sol Jung, Young Jae Cho, Minseok Suh, Wooil Kwon, Yun-Sang Lee, Gi Jeong Cheon, Jin-Young Jang","doi":"10.1007/s11307-024-01950-w","DOIUrl":"https://doi.org/10.1007/s11307-024-01950-w","url":null,"abstract":"<p><strong>Purpose: </strong>Accurate clinical staging of potentially resectable pancreatic ductal adenocarcinoma (PDAC) is critical for establishing optimal treatment strategies. While the efficacy of fluorine-18-fluorodeoxyglucose ([<sup>18</sup>F]FDG) positron emission tomography/computed tomography (PET/CT) in clinical staging is unclear, PET/CT detecting fibroblast-activation protein (FAP) expression has recently received considerable attention for detecting various tumors, including PDAC, with high sensitivity. We explored the efficacy of [<sup>18</sup>F]FDG and [<sup>18</sup>F]AIF-FAPI-74 PET/CT in the initial evaluation of potentially resectable PDAC.</p><p><strong>Procedures: </strong>Between 2021 and 2022, twenty participants with newly diagnosed potentially resectable PDAC were enrolled. After the initial evaluation with pancreatic CT, [<sup>18</sup>F]FDG PET/CT, and [<sup>18</sup>F]AIF-FAPI-74 PET/CT, treatment strategies were determined considering the participant's general status, clinical staging, and resectability. Pathological information from the surgical specimens was only available in 17 participants who underwent curative-intent surgery. Head-to-head comparisons of quantitative radiotracer uptake and diagnostic performance were performed among imaging modalities.</p><p><strong>Results: </strong>[<sup>18</sup>F]AIF-FAPI-74 PET/CT showed a significantly higher maximum standardized uptake value than [<sup>18</sup>F]FDG PET/CT did in evaluating primary pancreatic lesions (median [interquartile range]; 12.6 [10.7-13.7] vs. 6.3 [4.8-9.2]; P < 0.001). In contrast, [<sup>18</sup>F]AIF-FAPI-74 PET/CT showed a significantly lower mean standardized uptake value than [<sup>18</sup>F]FDG PET/CT did in evaluating background organ (median [interquartile range]) 0.8 [0.7-0.9] vs. 2.6 [2.3-2.7]; P < 0.001). In addition, the sensitivity of [<sup>18</sup>F]AIF-FAPI-74 PET/CT in detecting metastatic lymph nodes was higher than that of [<sup>18</sup>F]FDG PET/CT (50.0% vs. 0.0%; P = 0.026).</p><p><strong>Conclusion: </strong>This study demonstrated that [<sup>18</sup>F]AIF-FAPI-74 PET/CT could improve the clinical staging of potentially resectable PDAC.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01DOI: 10.1007/s11307-024-01949-3
Benjamin I Leach, Deanne Lister, Stephen R Adams, Julie Bykowski, Amy B Schwartz, Patrick McConville, Hemi Dimant, Eric T Ahrens
{"title":"Correction: Cryo-Fluorescence Tomography as a Tool for Visualizing Whole-Body Inflammation Using Perfluorocarbon Nanoemulsion Tracers.","authors":"Benjamin I Leach, Deanne Lister, Stephen R Adams, Julie Bykowski, Amy B Schwartz, Patrick McConville, Hemi Dimant, Eric T Ahrens","doi":"10.1007/s11307-024-01949-3","DOIUrl":"10.1007/s11307-024-01949-3","url":null,"abstract":"","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142080925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01Epub Date: 2024-08-28DOI: 10.1007/s11307-024-01946-6
Rong Xue, Zhixi Liu, Liang Liu, Shufen Sun, Zheli Gong
Purpose: This study aimed to develop a novel method for real-time imaging to track macrophages and to make it possible to visually track their dynamic features.
Procedures: The archaeon Halobacterium NRC-1 was cultured in an ATCC medium. Buoyant cells were allowed to produce biosynthetic gas vesicles (GVs), and isolated GVs were collected after lysis. Gas vesicle-labelled macrophages (GV@RAWs) were obtained by incubating macrophage (RAW 264.7) cells with GVs. The ability of GV@RAWs to track macrophages in real-time for a long term was assessed using a high-frequency ultrasound imaging system.
Results: We successfully synthesised and isolated GV@RAWs by co-incubating them with RAW 264.7. The results showed that GV@RAW produced significant ultrasound signals without affecting cell survival and could achieve real-time imaging for up to 3 days in vitro.
Conclusion: This research provides a new way to achieve long-term real-time imaging of macrophages, opening up new possibilities for immune response research, clinical diagnosis and therapeutic strategies for inflammatory diseases.
{"title":"Ultrasound Imaging of Macrophages Intracellularly Labelled with Biosynthetic Gas Vesicles.","authors":"Rong Xue, Zhixi Liu, Liang Liu, Shufen Sun, Zheli Gong","doi":"10.1007/s11307-024-01946-6","DOIUrl":"10.1007/s11307-024-01946-6","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to develop a novel method for real-time imaging to track macrophages and to make it possible to visually track their dynamic features.</p><p><strong>Procedures: </strong>The archaeon Halobacterium NRC-1 was cultured in an ATCC medium. Buoyant cells were allowed to produce biosynthetic gas vesicles (GVs), and isolated GVs were collected after lysis. Gas vesicle-labelled macrophages (GV@RAWs) were obtained by incubating macrophage (RAW 264.7) cells with GVs. The ability of GV@RAWs to track macrophages in real-time for a long term was assessed using a high-frequency ultrasound imaging system.</p><p><strong>Results: </strong>We successfully synthesised and isolated GV@RAWs by co-incubating them with RAW 264.7. The results showed that GV@RAW produced significant ultrasound signals without affecting cell survival and could achieve real-time imaging for up to 3 days in vitro.</p><p><strong>Conclusion: </strong>This research provides a new way to achieve long-term real-time imaging of macrophages, opening up new possibilities for immune response research, clinical diagnosis and therapeutic strategies for inflammatory diseases.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142093557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01Epub Date: 2024-07-26DOI: 10.1007/s11307-024-01932-y
Uttam M Shrestha, Hee-Don Chae, Qizhi Fang, Randall J Lee, Juliet Packiasamy, Lyna Huynh, Joseph Blecha, Tony L Huynh, Henry F VanBrocklin, Jelena Levi, Youngho Seo
Purpose: Myocardial infarction (MI) with subsequent inflammation is one of the most common heart conditions leading to progressive tissue damage. A reliable imaging marker to assess tissue viability after MI would help determine the risks and benefits of any intervention. In this study, we investigate whether a new mitochondria-targeted imaging agent, 18F-labeled 2'-deoxy-2'-18F-fluoro-9-β-d-arabinofuranosylguanine ([18F]F-AraG), a positron emission tomography (PET) agent developed for imaging activated T cells, is suitable for cardiac imaging and to test the myocardial viability after MI.
Procedure: To test whether the myocardial [18F]-F-AraG signal is coming from cardiomyocytes or immune infiltrates, we compared cardiac signal in wild-type (WT) mice with that of T cell deficient Rag1 knockout (Rag1 KO) mice. We assessed the effect of dietary nucleotides on myocardial [18F]F-AraG uptake in normal heart by comparing [18F]F-AraG signals between mice fed with purified diet and those fed with purified diet supplemented with nucleotides. The myocardial viability was investigated in rodent model by imaging rat with [18F]F-AraG and 2-deoxy-2[18F]fluoro-D-glucose ([18F]FDG) before and after MI. All PET signals were quantified in terms of the percent injected dose per cc (%ID/cc). We also explored [18F]FDG signal variability and potential T cell infiltration into fibrotic area in the affected myocardium with H&E analysis.
Results: The difference in %ID/cc for Rag1 KO and WT mice was not significant (p = ns) indicating that the [18F]F-AraG signal in the myocardium was primarily coming from cardiomyocytes. No difference in myocardial uptake was observed between [18F]F-AraG signals in mice fed with purified diet and with purified diet supplemented with nucleotides (p = ns). The [18F]FDG signals showed wider variability at different time points. Noticeable [18F]F-AraG signals were observed in the affected MI regions. There were T cells in the fibrotic area in the H&E analysis, but they did not constitute the predominant infiltrates.
Conclusions: Our preliminary preclinical data show that [18F]F-AraG accumulates in cardiomyocytes indicating that it may be suitable for cardiac imaging and to evaluate the myocardial viability after MI.
目的:心肌梗塞(MI)及随后的炎症是导致组织逐渐损伤的最常见心脏疾病之一。评估心肌梗死后组织存活能力的可靠成像标记物有助于确定任何干预措施的风险和益处。在这项研究中,我们研究了一种新的线粒体靶向成像剂--18F标记的2'-脱氧-2'-18F-氟-9-β-d-阿拉伯呋喃糖基鸟嘌呤([18F]F-AraG)--一种为活化T细胞成像而开发的正电子发射断层扫描(PET)剂,是否适用于心脏成像和测试心肌梗死后的心肌活力:为了检测心肌[18F]-F-AraG信号是来自心肌细胞还是免疫浸润,我们比较了野生型(WT)小鼠和T细胞缺陷Rag1基因敲除(Rag1 KO)小鼠的心肌信号。我们通过比较以纯净饮食喂养的小鼠和以补充核苷酸的纯净饮食喂养的小鼠的[18F]F-AraG 信号,评估了饮食中的核苷酸对正常心脏心肌[18F]F-AraG 摄取的影响。在啮齿动物模型中,用[18F]F-AraG 和 2-脱氧-2[18F]氟-D-葡萄糖([18F]FDG)对心肌梗死前后的大鼠进行成像,研究心肌活力。所有 PET 信号均以每毫升注射剂量百分比(%ID/cc)进行量化。我们还通过 H&E 分析探讨了[18F]FDG 信号的可变性以及 T 细胞向受累心肌纤维化区域浸润的可能性:结果:Rag1 KO 和 WT 小鼠的 %ID/cc 差异不显著(p = ns),表明心肌中的 [18F]F-AraG 信号主要来自心肌细胞。用纯化饮食和补充核苷酸的纯化饮食喂养的小鼠,其心肌摄取的[18F]F-AraG 信号没有差异(p = ns)。在不同的时间点,[18F]FDG 信号的变化幅度更大。在受影响的 MI 区域观察到明显的 [18F]F-AraG 信号。H&E分析显示,纤维化区域存在T细胞,但它们并不构成主要浸润:我们的初步临床前数据显示,[18F]F-AraG 可在心肌细胞中积聚,这表明它可能适用于心脏成像和心肌梗死后心肌存活能力的评估。
{"title":"A Feasibility Study of [<sup>18</sup>F]F-AraG Positron Emission Tomography (PET) for Cardiac Imaging-Myocardial Viability in Ischemia-Reperfusion Injury Model.","authors":"Uttam M Shrestha, Hee-Don Chae, Qizhi Fang, Randall J Lee, Juliet Packiasamy, Lyna Huynh, Joseph Blecha, Tony L Huynh, Henry F VanBrocklin, Jelena Levi, Youngho Seo","doi":"10.1007/s11307-024-01932-y","DOIUrl":"10.1007/s11307-024-01932-y","url":null,"abstract":"<p><strong>Purpose: </strong>Myocardial infarction (MI) with subsequent inflammation is one of the most common heart conditions leading to progressive tissue damage. A reliable imaging marker to assess tissue viability after MI would help determine the risks and benefits of any intervention. In this study, we investigate whether a new mitochondria-targeted imaging agent, <sup>18</sup>F-labeled 2'-deoxy-2'-<sup>18</sup>F-fluoro-9-β-d-arabinofuranosylguanine ([<sup>18</sup>F]F-AraG), a positron emission tomography (PET) agent developed for imaging activated T cells, is suitable for cardiac imaging and to test the myocardial viability after MI.</p><p><strong>Procedure: </strong>To test whether the myocardial [<sup>18</sup>F]-F-AraG signal is coming from cardiomyocytes or immune infiltrates, we compared cardiac signal in wild-type (WT) mice with that of T cell deficient Rag1 knockout (Rag1 KO) mice. We assessed the effect of dietary nucleotides on myocardial [<sup>18</sup>F]F-AraG uptake in normal heart by comparing [<sup>18</sup>F]F-AraG signals between mice fed with purified diet and those fed with purified diet supplemented with nucleotides. The myocardial viability was investigated in rodent model by imaging rat with [<sup>18</sup>F]F-AraG and 2-deoxy-2[<sup>18</sup>F]fluoro-D-glucose ([<sup>18</sup>F]FDG) before and after MI. All PET signals were quantified in terms of the percent injected dose per cc (%ID/cc). We also explored [<sup>18</sup>F]FDG signal variability and potential T cell infiltration into fibrotic area in the affected myocardium with H&E analysis.</p><p><strong>Results: </strong>The difference in %ID/cc for Rag1 KO and WT mice was not significant (p = ns) indicating that the [<sup>18</sup>F]F-AraG signal in the myocardium was primarily coming from cardiomyocytes. No difference in myocardial uptake was observed between [<sup>18</sup>F]F-AraG signals in mice fed with purified diet and with purified diet supplemented with nucleotides (p = ns). The [<sup>18</sup>F]FDG signals showed wider variability at different time points. Noticeable [<sup>18</sup>F]F-AraG signals were observed in the affected MI regions. There were T cells in the fibrotic area in the H&E analysis, but they did not constitute the predominant infiltrates.</p><p><strong>Conclusions: </strong>Our preliminary preclinical data show that [<sup>18</sup>F]F-AraG accumulates in cardiomyocytes indicating that it may be suitable for cardiac imaging and to evaluate the myocardial viability after MI.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141766811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01Epub Date: 2024-07-31DOI: 10.1007/s11307-024-01937-7
Emil Novruzov, Mardjan Dabir, Dominik Schmitt, Katalin Mattes-György, Markus Beu, Yuriko Mori, Christina Antke, Sebastian Reinartz, Artur Lichtenberg, Gerald Antoch, Frederik L Giesel, Hug Aubin, Eduards Mamlins
Purpose: Left ventricular assisting device (LVAD) is a vital mechanical circulatory assist device for patients with end-stage heart disease, serving as either a bridge to transplantation or palliative destination therapy. Yet device infection represents a major lethal complication, warranting a multi-step, complex therapy approach including an urgent device exchange or heart transplantation. Still, timely diagnosis of site and extent of VAD-specific infection for a proper therapy planning poses challenges in regular clinical care. This single-center, retrospective study aimed to evaluate the impact of volumetric PET parameters with different thresholding compared to semiquantitative PET parameters for accurate diagnosis of VAD-specific infection.
Procedures: Seventeen patients (1 female, 16 males; mean age 57 ± 11 years) underwent [18F]FDG imaging for suspected VAD-specific infection between April 2013 and October 2023. Various metabolic and volumetric PET parameters with different thresholding were collected for specific LVAD components including driveline entry point, subcutaneous driveline, pump pocket, inner cannula and outflow tract. Microbiology and clinical follow-up were used as the final diagnosis standard.
Results: Nine of eleven patients with VAD-specific infection underwent urgent heart transplantation, and one had a surgical revision of LVAD. Two patients had non-VAD specific infections, and two had non-VAD related infections. Metabolic burden determination using a fixed absolute threshold provided the best outcome compared to relative thresholding or other metabolic SUV parameters. The total metabolic tumor volume (MTV) cutoff value was 9.3 cm3, and the corresponding sensitivity, specificity, accuracy, and AUC were 90.0%, 71.43%, 82.5%, and 0.814 (95% CI 0.555-0.958), respectively. The total lesion glycolysis (TLG) was 30.6, and the corresponding sensitivity, specificity, accuracy, and AUC were 90.0%, 71.4%, 82.5%, and 0.829 (95% CI 0.571-0.964), respectively.
Conclusions: Volumetric PET parameters with fixed absolute thresholding appear to be a valuable auxiliary tool in the evaluation of [18F]FDG imaging to enhance the diagnostic accuracy of VAD-specific infection.
目的:对于终末期心脏病患者来说,左心室辅助装置(LVAD)是一种重要的机械循环辅助装置,既可作为移植的桥梁,也可作为姑息治疗的终点。然而,装置感染是一种主要的致命并发症,需要采取多步骤、复杂的治疗方法,包括紧急装置交换或心脏移植。然而,在常规临床护理中,及时诊断 VAD 感染的部位和程度以制定正确的治疗计划仍是一项挑战。这项单中心回顾性研究旨在评估不同阈值的容积 PET 参数与半定量 PET 参数相比对准确诊断 VAD 特异性感染的影响:17 名患者(1 名女性,16 名男性;平均年龄 57 ± 11 岁)在 2013 年 4 月至 2023 年 10 月期间因疑似 VAD 特异性感染接受了[18F]FDG 成像检查。针对特定的 LVAD 组件(包括动力线入口、皮下动力线、泵袋、内套管和流出道)采集了不同阈值的各种代谢和容积 PET 参数。微生物学和临床随访作为最终诊断标准:结果:在11例VAD特异性感染患者中,有9例接受了紧急心脏移植手术,1例进行了LVAD手术修补。两名患者发生了非 VAD 特异性感染,两名患者发生了与 VAD 无关的感染。与相对阈值或其他代谢 SUV 参数相比,使用固定绝对阈值确定代谢负荷的结果最好。总代谢肿瘤体积 (MTV) 临界值为 9.3 立方厘米,相应的敏感性、特异性、准确性和 AUC 分别为 90.0%、71.43%、82.5% 和 0.814(95% CI 0.555-0.958)。总病变糖酵解(TLG)为 30.6,相应的敏感性、特异性、准确性和 AUC 分别为 90.0%、71.4%、82.5% 和 0.829(95% CI 0.571-0.964):具有固定绝对阈值的容积 PET 参数似乎是评估[18F]FDG 成像的一种有价值的辅助工具,可提高 VAD 特异性感染的诊断准确性。
{"title":"The Predictive Role of Metabolic Volume Segmentation Compared to Semiquantitative PET Parameters in Diagnosis of LVAD Infection using [<sup>18</sup>F]FDG Imaging.","authors":"Emil Novruzov, Mardjan Dabir, Dominik Schmitt, Katalin Mattes-György, Markus Beu, Yuriko Mori, Christina Antke, Sebastian Reinartz, Artur Lichtenberg, Gerald Antoch, Frederik L Giesel, Hug Aubin, Eduards Mamlins","doi":"10.1007/s11307-024-01937-7","DOIUrl":"10.1007/s11307-024-01937-7","url":null,"abstract":"<p><strong>Purpose: </strong>Left ventricular assisting device (LVAD) is a vital mechanical circulatory assist device for patients with end-stage heart disease, serving as either a bridge to transplantation or palliative destination therapy. Yet device infection represents a major lethal complication, warranting a multi-step, complex therapy approach including an urgent device exchange or heart transplantation. Still, timely diagnosis of site and extent of VAD-specific infection for a proper therapy planning poses challenges in regular clinical care. This single-center, retrospective study aimed to evaluate the impact of volumetric PET parameters with different thresholding compared to semiquantitative PET parameters for accurate diagnosis of VAD-specific infection.</p><p><strong>Procedures: </strong>Seventeen patients (1 female, 16 males; mean age 57 ± 11 years) underwent [<sup>18</sup>F]FDG imaging for suspected VAD-specific infection between April 2013 and October 2023. Various metabolic and volumetric PET parameters with different thresholding were collected for specific LVAD components including driveline entry point, subcutaneous driveline, pump pocket, inner cannula and outflow tract. Microbiology and clinical follow-up were used as the final diagnosis standard.</p><p><strong>Results: </strong>Nine of eleven patients with VAD-specific infection underwent urgent heart transplantation, and one had a surgical revision of LVAD. Two patients had non-VAD specific infections, and two had non-VAD related infections. Metabolic burden determination using a fixed absolute threshold provided the best outcome compared to relative thresholding or other metabolic SUV parameters. The total metabolic tumor volume (MTV) cutoff value was 9.3 cm<sup>3</sup>, and the corresponding sensitivity, specificity, accuracy, and AUC were 90.0%, 71.43%, 82.5%, and 0.814 (95% CI 0.555-0.958), respectively. The total lesion glycolysis (TLG) was 30.6, and the corresponding sensitivity, specificity, accuracy, and AUC were 90.0%, 71.4%, 82.5%, and 0.829 (95% CI 0.571-0.964), respectively.</p><p><strong>Conclusions: </strong>Volumetric PET parameters with fixed absolute thresholding appear to be a valuable auxiliary tool in the evaluation of [<sup>18</sup>F]FDG imaging to enhance the diagnostic accuracy of VAD-specific infection.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11436428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}