Chain forming diatoms use different strategies to avoid diffusion limited N assimilation

IF 3.8 1区 地球科学 Q1 LIMNOLOGY Limnology and Oceanography Pub Date : 2024-08-30 DOI:10.1002/lno.12677
Rickard Stenow, Malin Olofsson, Martin J. Whitehouse, Helle Ploug
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Abstract

Formation of large colonies by phytoplankton is considered a disadvantage during low nutrient and non-turbulent conditions because of diffusion limitation and competition by neighboring cells. This is assumed by diffusion models and not empirical measurements. Here, we measured cell-specific dissolved inorganic carbon (DIC) and nitrate ( NO 3 ) assimilation in two chain-forming Skeletonema marinoi strains by combining secondary ion mass spectrometry with stable isotopic tracer incubations. The pelagic strains were recently germinated from resting stages and thus not adapted to high nutrient regimes of laboratory cultures. During the exponential phase, the cells assimilated excess NO 3 relative to DIC. The DIC and NO 3 assimilation varied with chain length or the position of the cell within chains but did not follow any consistent trend. Solitary cells were rare and did not exceed 6% of all cells. During the nitrogen-limited stationary phase, NO 3 assimilation was lower than modeled by mass transfer theory at diffusion limitation. NO 3 assimilation rates were apparently limited by the biological uptake rate at nitrate concentrations < 0.46 μmol L−1. We conclude that S. marinoi is adapted to high nutrient concentrations and may seldom be diffusion limited in coastal waters with ambient NO 3 concentrations > 0.46 μmol L−1. These findings contrast those previously reported in larger chain forming diatoms, for example, Chaetoceros, which appear to circumvent diffusion limitation at low ambient NO 3 concentrations and succeed S. marinoi during spring blooms.

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成链硅藻使用不同的策略来避免扩散受限的氮同化作用
在低营养和非湍流条件下,浮游植物形成大型菌落被认为是一种不利因素,原因是扩散受限和邻近细胞的竞争。这是扩散模型的假设,而不是经验测量的结果。在此,我们通过将二次离子质谱法与稳定同位素示踪培养法相结合,测量了两株成链海鞘(Skeletonema marinoi)细胞特异性溶解无机碳(DIC)和硝酸盐()同化作用。浮游菌株最近才从静止阶段发芽,因此不适应实验室培养的高营养机制。在指数期,细胞同化过量的 DIC。DIC 和同化量随链的长度或细胞在链中的位置而变化,但没有任何一致的趋势。单细胞很少见,不超过所有细胞的 6%。在氮限制静止期,同化率低于扩散限制下的传质理论模型。在硝酸盐浓度为 0.46 μmol L-1 时,同化率显然受到生物吸收率的限制。我们的结论是,S. marinoi 能适应高浓度的营养物质,在环境浓度为 0.46 μmol L-1 的沿岸水域可能很少受到扩散限制。这些发现与之前报道的较大的成链硅藻(如 Chaetoceros)的情况不同,后者似乎能在环境浓度较低时规避扩散限制,并在春季硅藻大量繁殖时接替 S. marinoi。
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来源期刊
Limnology and Oceanography
Limnology and Oceanography 地学-海洋学
CiteScore
8.80
自引率
6.70%
发文量
254
审稿时长
3 months
期刊介绍: Limnology and Oceanography (L&O; print ISSN 0024-3590, online ISSN 1939-5590) publishes original articles, including scholarly reviews, about all aspects of limnology and oceanography. The journal''s unifying theme is the understanding of aquatic systems. Submissions are judged on the originality of their data, interpretations, and ideas, and on the degree to which they can be generalized beyond the particular aquatic system examined. Laboratory and modeling studies must demonstrate relevance to field environments; typically this means that they are bolstered by substantial "real-world" data. Few purely theoretical or purely empirical papers are accepted for review.
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