ZmCRK1 negatively regulates maize's response to drought stress by phosphorylating plasma membrane H+-ATPase ZmMHA2

IF 8.3 1区生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2024-09-01 DOI:10.1111/nph.20093

Jinjie Liu, Xi-Dong Li, Dongyun Jia, Liuran Qi, Rufan Jing, Jie Hao, Zhe Wang, Jinkui Cheng, Li-Mei Chen

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Abstract

Drought severely affects crop growth and yields. Stomatal regulation plays an important role in plant response to drought stress. Light-activated plasma membrane-localized proton ATPase (PM H⁺-ATPase) mainly promoted the stomatal opening. Abscisic acid (ABA) plays a dominant role in the stomatal closure during drought stress. It is not clear how PM H⁺-ATPase is involved in the regulation of ABA-induced stomatal closure.
We found that a CALCIUM-DEPENDENT PROTEIN KINASE RELATED KINASE 1 (ZmCRK1), and its mutant zmcrk1 exhibited slow water loss in detached leaves, high-survival rate after drought stress, and sensitivity to stomatal closure induced by ABA. The ZmCRK1 overexpression lines are opposite.
ZmCRK1 interacted with the maize PM H⁺-ATPase ZmMHA2. ZmCRK1 phosphorylated ZmMHA2 at the Ser-901 and inhibited its proton pump activity. ZmCRK1 overexpression lines and zmmha2 mutants had low H⁺-ATPase activity, resulting in impaired ABA-induced H⁺ efflux.
Taken together, our study indicates that ZmCRK1 negatively regulates maize drought stress response by inhibiting the activity of ZmMHA2. Reducing the expression level of ZmCRK1 has the potential to reduce yield losses under water deficiency.

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ZmCRK1通过磷酸化质膜H+-ATP酶ZmMHA2负向调节玉米对干旱胁迫的响应。

干旱严重影响作物的生长和产量。气孔调节在植物应对干旱胁迫中起着重要作用。光激活的质膜定位质子ATP酶（PM H+-ATP酶）主要促进气孔开放。脱落酸（ABA）在干旱胁迫期间的气孔关闭中起主导作用。目前还不清楚 PM H+-ATPase 如何参与调控 ABA 诱导的气孔关闭。我们发现，钙独立蛋白激酶相关激酶1（ZmCRK1）及其突变体zmcrk1在离体叶片中表现出失水慢、干旱胁迫后存活率高以及对ABA诱导的气孔关闭敏感等特性。ZmCRK1过表达株则相反。ZmCRK1 与玉米 PM H+-ATP 酶 ZmMHA2 相互作用。ZmCRK1 在 Ser-901 处磷酸化 ZmMHA2 并抑制其质子泵活性。ZmCRK1过表达株系和zmmha2突变体的H+-ATP酶活性较低，导致ABA诱导的H+外流受损。综上所述，我们的研究表明 ZmCRK1 通过抑制 ZmMHA2 的活性来负向调节玉米干旱胁迫响应。降低 ZmCRK1 的表达水平有可能减少缺水情况下的产量损失。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.